##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545423_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5001419
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38501873168395	31.0	31.0	33.0	30.0	34.0
2	31.73075601144395	31.0	31.0	34.0	30.0	34.0
3	31.80573433259641	31.0	31.0	34.0	30.0	34.0
4	35.5024444062775	37.0	35.0	37.0	33.0	37.0
5	35.36647879331846	37.0	35.0	37.0	33.0	37.0
6	35.43108645766331	37.0	35.0	37.0	33.0	37.0
7	35.8927944249422	37.0	35.0	37.0	35.0	37.0
8	35.934789506737985	37.0	35.0	37.0	35.0	37.0
9	37.60020066305182	39.0	37.0	39.0	35.0	39.0
10	37.02151389435678	39.0	37.0	39.0	33.0	39.0
11	36.63167613031422	39.0	35.0	39.0	32.0	39.0
12	35.68591573711381	37.0	35.0	39.0	31.0	39.0
13	35.238047242192664	37.0	35.0	39.0	30.0	39.0
14	36.18477436103634	38.0	35.0	40.0	31.0	41.0
15	36.461822534764636	38.0	35.0	40.0	31.0	41.0
16	36.675039823698036	38.0	35.0	40.0	32.0	41.0
17	36.627887805440814	38.0	35.0	40.0	32.0	41.0
18	36.559244886301265	38.0	35.0	40.0	31.0	41.0
19	36.4692162364321	37.0	35.0	40.0	31.0	41.0
20	36.308440664539404	37.0	35.0	40.0	31.0	41.0
21	36.116510334367106	37.0	35.0	40.0	31.0	41.0
22	36.040296163948675	37.0	35.0	40.0	31.0	41.0
23	36.017171726663975	36.0	34.0	40.0	31.0	41.0
24	35.94500820667095	36.0	34.0	40.0	31.0	41.0
25	35.825314175836894	36.0	34.0	40.0	30.0	41.0
26	35.72623769374252	36.0	34.0	40.0	30.0	41.0
27	35.67490866092203	36.0	34.0	40.0	30.0	41.0
28	35.700424219606475	36.0	34.0	40.0	30.0	41.0
29	35.7297486973197	36.0	34.0	40.0	30.0	41.0
30	35.651000246130145	36.0	34.0	40.0	30.0	41.0
31	35.46041873316353	36.0	34.0	40.0	30.0	41.0
32	35.22266980630897	36.0	34.0	40.0	29.0	41.0
33	35.02683518417473	36.0	34.0	40.0	28.0	41.0
34	34.82460777631308	35.0	34.0	40.0	27.0	41.0
35	34.663455711269144	35.0	34.0	40.0	26.0	41.0
36	34.46735696409359	35.0	33.0	40.0	25.0	41.0
37	34.388215624405795	35.0	33.0	40.0	24.0	41.0
38	34.41805735532256	35.0	33.0	40.0	25.0	41.0
39	34.34349851512141	35.0	33.0	40.0	24.0	41.0
40	34.15533251663178	35.0	33.0	40.0	23.0	41.0
41	34.17175665546118	35.0	33.0	40.0	24.0	41.0
42	34.10115589195786	35.0	33.0	40.0	24.0	41.0
43	33.99796597725565	35.0	33.0	40.0	23.0	41.0
44	33.922256463615625	35.0	33.0	40.0	23.0	41.0
45	33.85307649689018	35.0	33.0	40.0	23.0	41.0
46	33.86067234118957	35.0	33.0	39.0	23.0	41.0
47	33.82943540623171	35.0	33.0	39.0	23.0	41.0
48	33.81430669975861	35.0	33.0	39.0	23.0	41.0
49	33.77653301992894	35.0	33.0	39.0	24.0	41.0
50	33.62725318554594	35.0	33.0	39.0	24.0	41.0
51	33.4539845591821	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	5.0
13	7.0
14	38.0
15	122.0
16	411.0
17	1220.0
18	2605.0
19	5296.0
20	9185.0
21	14973.0
22	22756.0
23	32471.0
24	46910.0
25	66077.0
26	85707.0
27	99628.0
28	108382.0
29	122412.0
30	145055.0
31	177294.0
32	222466.0
33	290985.0
34	509432.0
35	816067.0
36	341765.0
37	398529.0
38	555183.0
39	925761.0
40	673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.371940443302194	24.556490867891693	30.04671274292356	15.024855945882559
2	30.215444856749656	26.75764618001411	29.955598601117	13.071310362119231
3	28.639232185905637	24.713026443095448	32.696360772812675	13.951380598186237
4	25.414707306066536	27.367992963596933	32.53712596365151	14.680173766685014
5	22.776915911264382	32.24940761811798	31.270325481628312	13.703350988989325
6	21.197524142648316	39.769753344000975	28.313844530922122	10.718877982428587
7	84.99969708596701	5.2036632003837315	7.947884390410001	1.8487553232392646
8	86.63575277336292	3.9712929470616243	7.2707365649628635	2.122217714612593
9	81.45606276938605	6.0773152579298	9.54769036547428	2.918931607209874
10	53.060841333229625	20.42246410468709	15.376916031230337	11.139778530852944
11	47.40138748623141	19.62990903181677	20.13412593505963	12.834577546892193
12	42.04792679837462	20.638962662396413	24.134630591838036	13.178479947390931
13	20.89063123885441	40.722742885569076	24.233962401470464	14.152663474106047
14	15.103073747670411	42.756085822843474	26.984821707599384	15.15601872188673
15	14.110835344929109	22.620920182852107	49.32782076446705	13.940423707751739
16	15.102493912227711	18.261937262204984	48.02846952035013	18.60709930521718
17	15.999079461248897	18.57024976311723	29.408193954555696	36.02247682107817
18	20.324112017009575	23.330078923601484	36.11281118418593	20.23299787520302
19	29.427628439049	23.52446375718571	26.862256491607685	20.18565131215761
20	31.83906407361591	21.533248863972403	25.736176073230414	20.89151098918127
21	21.442494620026835	28.03766291126578	29.096862310476286	21.422980158231095
22	21.38795009976169	25.379697241922745	24.974072358264724	28.25828030005085
23	19.240159642693403	29.732801830840405	25.490645754734807	25.536392771731382
24	19.72832110247112	22.96968120447417	40.47455332176728	16.827444371287427
25	16.70861809418487	24.35184894526933	37.80533084710559	21.134202113440207
26	16.4202599302318	34.15298738218094	28.94078660476157	20.485966082825694
27	17.319184815349402	35.36038472281567	28.618278132665946	18.70215232916898
28	14.891893680573453	29.675658048245907	38.533304248254346	16.899144022926293
29	15.046469811867391	23.946204067285702	38.28971337934294	22.717612741503963
30	17.72834869464046	31.28792048816546	32.44737143598647	18.53635938120761
31	28.509988865160068	27.170449026566263	25.840266532358118	18.479295575915554
32	29.41151301260702	25.71926087376403	28.109482528858308	16.759743584770643
33	29.39829676337855	27.70731666353089	24.615414145465518	18.27897242762504
34	20.13070690538025	27.869570615859217	28.009950775969777	23.989771702790748
35	20.464612143073797	25.7638482198752	31.36373897088006	22.407800666170942
36	30.205227756362746	25.568163755126292	26.404186491873606	17.822421996637356
37	19.89225457815072	33.93736857479847	29.69265322501474	16.477723622036066
38	21.069820384974744	33.09046892491911	24.41251172917126	21.427198960934888
39	20.8082546173396	32.900682786225275	27.552580577632067	18.73848201880306
40	24.248018412374567	26.518574028690654	26.502578568202345	22.73082899073243
41	16.833682600877868	25.66093742595851	27.394265507448985	30.11111446571463
42	21.086915533371627	25.66065750539997	25.275706754423094	27.97672020680531
43	22.130399392652365	25.531214241398292	26.33920493363983	25.99918143230951
44	18.994649318523404	30.176615876414274	29.302543938030386	21.526190867031936
45	16.53538725709644	38.2359486377766	23.809562846064285	21.41910125906268
46	21.384011217616443	33.7542605408585	25.999701284775377	18.862026956749673
47	21.652634982192055	28.016308971513887	26.51107615658676	23.8199798897073
48	23.283772065487813	23.96227950507646	31.54534743039925	21.208600999036474
49	21.538827280817703	24.202531321610927	33.095107608460715	21.16353378911065
50	19.951557747911142	33.71147268405227	26.967546610272002	19.369422957764588
51	19.157383134666382	33.32398265372287	24.500106869670386	23.018527341940356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4169.0
1	7072.0
2	9975.0
3	26543.0
4	43111.0
5	31840.5
6	20570.0
7	21963.5
8	23357.0
9	25227.5
10	27098.0
11	28391.0
12	29684.0
13	29414.5
14	29145.0
15	28430.0
16	27715.0
17	26566.5
18	25418.0
19	25853.0
20	26288.0
21	24868.5
22	23449.0
23	23518.5
24	23588.0
25	27259.5
26	38963.5
27	46996.0
28	54750.5
29	62505.0
30	72490.0
31	82475.0
32	96499.5
33	110524.0
34	120620.0
35	130716.0
36	134786.5
37	138857.0
38	166497.5
39	194138.0
40	273016.5
41	351895.0
42	437984.0
43	524073.0
44	534703.5
45	545334.0
46	517140.0
47	488946.0
48	432001.0
49	375056.0
50	341432.0
51	307808.0
52	298472.0
53	289136.0
54	253596.5
55	218057.0
56	195913.5
57	173770.0
58	164205.0
59	154640.0
60	145708.0
61	136776.0
62	118705.0
63	100634.0
64	87747.0
65	74860.0
66	62632.0
67	50404.0
68	41642.0
69	32880.0
70	28875.0
71	24870.0
72	21433.5
73	17997.0
74	14654.0
75	8389.0
76	5467.0
77	4200.5
78	2934.0
79	2634.5
80	2335.0
81	1686.0
82	1037.0
83	651.5
84	266.0
85	191.5
86	117.0
87	88.0
88	59.0
89	37.0
90	15.0
91	15.5
92	16.0
93	12.0
94	8.0
95	8.0
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5001419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.97662214969515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28653748136075	11.081579759346214
2	8.881140628597835	2.4825669364843606
3	3.2221472592570093	1.351042042599331
4	1.7029658329789168	0.9520683992554871
5	1.019185488057378	0.7122385233515307
6	0.6586633608198414	0.5523533350816348
7	0.4744945314510014	0.4642281544731102
8	0.36547239050210223	0.40864556065549745
9	0.2692455332360371	0.33868287751799503
>10	1.8908682639328087	5.58215423748076
>50	0.5493404379812475	5.630972094904273
>100	1.4545314574088488	48.469186803072404
>500	0.21253930826923123	18.348656116481703
>1k	0.012289687893118814	2.4858513932527337
>5k	2.8916912689691326E-4	0.2549041928453902
>10k+	2.8916912689691326E-4	0.8848695731975541
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25750	0.5148538844675881	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	18038	0.36065764536024675	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	7537	0.15069723212552277	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5077	0.10151119112395902	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05120546788821333	0.0
2	0.0	0.0	0.0	0.17609002565072032	0.0
3	0.0	0.0	0.0	0.24569027310049407	0.0
4	0.0	0.0	0.0	0.3799521695742748	0.0
5	0.0	0.0	0.0	0.6006295413361687	0.0
6	0.0	0.0	0.0	0.8857686188659658	0.0
7	0.0	0.0	0.0	1.0644779011716474	0.0
8	0.0	0.0	0.0	1.5335447799914383	0.0
9	0.0	0.0	0.0	1.721331486124238	0.0
10	0.0	0.0	0.0	2.032083294760947	0.0
11	0.0	0.0	0.0	2.332178127847317	0.0
12	0.0	0.0	0.0	2.584666471655344	0.0
13	0.0	0.0	0.0	2.6828186160767573	0.0
14	0.0	0.0	0.0	2.719288265990112	0.0
15	0.0	0.0	0.0	2.773033013230845	0.0
16	0.0	0.0	0.0	2.8982974631799494	0.0
17	0.0	0.0	0.0	3.044435989066303	0.0
18	0.0	0.0	0.0	3.247978223780091	1.999432561039177E-5
19	0.0	0.0	0.0	3.3468901525746992	1.999432561039177E-5
20	3.998865122078354E-5	0.0	0.0	3.449201116723074	1.999432561039177E-5
21	3.998865122078354E-5	0.0	0.0	3.5862222301310887	1.999432561039177E-5
22	3.998865122078354E-5	0.0	0.0	3.72510281582087	1.999432561039177E-5
23	3.998865122078354E-5	0.0	0.0	3.8870768475906536	1.999432561039177E-5
24	3.998865122078354E-5	0.0	0.0	4.009602074931134	1.999432561039177E-5
25	3.998865122078354E-5	0.0	0.0	4.107074412281794	1.999432561039177E-5
26	3.998865122078354E-5	0.0	0.0	4.20840565447526	1.999432561039177E-5
27	3.998865122078354E-5	0.0	0.0	4.304478389033193	1.999432561039177E-5
28	3.998865122078354E-5	0.0	0.0	4.402650527780216	1.999432561039177E-5
29	3.998865122078354E-5	0.0	0.0	4.511959505892228	1.999432561039177E-5
30	3.998865122078354E-5	0.0	0.0	4.66265673801775	1.999432561039177E-5
31	3.998865122078354E-5	0.0	0.0	4.796758679886648	1.999432561039177E-5
32	3.998865122078354E-5	0.0	0.0	4.9196837937393365	1.999432561039177E-5
33	3.998865122078354E-5	0.0	0.0	5.038849974377272	3.998865122078354E-5
34	3.998865122078354E-5	0.0	0.0	5.16483422004835	3.998865122078354E-5
35	3.998865122078354E-5	0.0	0.0	5.334726004759849	3.998865122078354E-5
36	3.998865122078354E-5	0.0	0.0	5.468068162255552	3.998865122078354E-5
37	3.998865122078354E-5	0.0	0.0	5.6083683450636705	3.998865122078354E-5
38	5.998297683117531E-5	0.0	0.0	5.741350604698386	3.998865122078354E-5
39	5.998297683117531E-5	0.0	0.0	5.8761523479636475	3.998865122078354E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	130	0.0	45.000004	1
CGTGCTA	65	0.0	45.000004	19
TACGCCC	20	7.0355565E-4	45.000004	35
TACGATA	20	7.0355565E-4	45.000004	27
CGCTAAG	35	1.2130295E-7	45.000004	28
GTACGAC	35	1.2130295E-7	45.000004	34
CTTACCG	20	7.0355565E-4	45.000004	1
GGTACGA	25	3.8926883E-5	45.0	7
CGAATCG	25	3.8926883E-5	45.0	36
TATAGCG	600	0.0	42.750004	1
TATATCG	85	0.0	42.35294	1
AATACGG	455	0.0	42.032967	2
TACCTAG	150	0.0	42.000004	1
ACGTAAG	150	0.0	42.000004	1
GCGTAAT	75	0.0	42.000004	8
AGTAACG	290	0.0	41.896553	1
GCGAAGT	65	0.0	41.538464	28
ATGCGTC	60	3.6379788E-12	41.250004	43
CGTTTTT	19685	0.0	40.816612	1
CCACGTA	45	1.9304935E-8	40.0	11
>>END_MODULE