FastQCFastQC Report
Thu 26 May 2016
SRR1545422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545422_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9216950
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1253191.359658021362815No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA322340.3497252344864624No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC135110.14658862204959341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT846100.043.2635041
CTTGACC62700.039.5813377
CGTAAGG17450.039.197712
TCGTAAG5900.038.8983041
TGCATTA58700.038.75213215
TCAACTT65050.038.289783
CATTAGA59600.038.280217
GTTTTTT1009700.037.699812
GCATTAG60850.037.64174316
CTCAACT67100.037.4888232
GCTCAAC68900.037.1952131
ACTTGAC66800.037.1519476
GCTACGA13150.036.95817610
GGGCGAT172900.036.957787
TAGGGAC62700.036.8540655
TGATTCG9900.036.8181815
CGAATAT13150.036.78707514
TAGCGGG49400.036.528343
TTGACCC65400.036.4678888
AGGGCGA90800.036.0545166
Produced by FastQC (version 0.11.5)