##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545422_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	9216950
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194279344034634	31.0	31.0	33.0	30.0	34.0
2	31.56001074108029	31.0	31.0	34.0	30.0	34.0
3	31.590764515376563	31.0	31.0	34.0	30.0	34.0
4	35.364760359988935	37.0	35.0	37.0	33.0	37.0
5	35.2523982445386	37.0	35.0	37.0	32.0	37.0
6	35.32058153727643	37.0	35.0	37.0	32.0	37.0
7	35.7839149610229	37.0	35.0	37.0	35.0	37.0
8	35.8057972539723	37.0	35.0	37.0	35.0	37.0
9	37.45169432404429	39.0	37.0	39.0	35.0	39.0
10	36.82913404108734	39.0	37.0	39.0	32.0	39.0
11	36.51056303874926	39.0	35.0	39.0	32.0	39.0
12	36.08075805987881	38.0	35.0	39.0	31.0	39.0
13	35.88945800942828	38.0	35.0	39.0	31.0	39.0
14	36.93525656534971	39.0	35.0	41.0	31.0	41.0
15	37.05855852532562	39.0	35.0	41.0	31.0	41.0
16	37.15037230320225	39.0	35.0	41.0	32.0	41.0
17	37.066879065200524	39.0	35.0	41.0	32.0	41.0
18	36.87793239629161	38.0	35.0	40.0	31.0	41.0
19	36.72883253136884	38.0	35.0	40.0	31.0	41.0
20	36.47329496199936	38.0	35.0	40.0	31.0	41.0
21	36.2790060703378	38.0	35.0	40.0	30.0	41.0
22	36.25597665171234	38.0	35.0	40.0	30.0	41.0
23	36.14965243383115	38.0	35.0	40.0	30.0	41.0
24	36.0621491925203	38.0	35.0	40.0	30.0	41.0
25	35.94212554044451	37.0	34.0	40.0	30.0	41.0
26	35.86461692859351	37.0	34.0	40.0	30.0	41.0
27	35.82597204064251	37.0	34.0	40.0	30.0	41.0
28	35.74930199252464	37.0	34.0	40.0	29.0	41.0
29	35.69567297207862	37.0	34.0	40.0	29.0	41.0
30	35.54911841769783	37.0	34.0	40.0	29.0	41.0
31	35.32854414963735	37.0	34.0	40.0	27.0	41.0
32	35.02443020738965	37.0	34.0	40.0	26.0	41.0
33	34.67309370236358	37.0	33.0	40.0	24.0	41.0
34	34.2880366064696	37.0	33.0	40.0	23.0	41.0
35	34.01765128377609	37.0	33.0	40.0	21.0	41.0
36	33.83846619543341	37.0	33.0	40.0	21.0	41.0
37	33.75605357520655	37.0	33.0	40.0	20.0	41.0
38	33.66927302415658	36.0	33.0	40.0	19.0	41.0
39	33.533814222709246	36.0	33.0	40.0	18.0	41.0
40	33.37100797986319	36.0	32.0	40.0	18.0	41.0
41	33.250977384058714	36.0	32.0	40.0	18.0	41.0
42	33.12520389065797	36.0	32.0	40.0	18.0	41.0
43	33.02336206662725	35.0	32.0	40.0	18.0	41.0
44	32.92103287964023	35.0	32.0	40.0	17.0	41.0
45	32.876153608297756	35.0	31.0	40.0	18.0	41.0
46	32.83013947130016	35.0	31.0	40.0	18.0	41.0
47	32.779597155241156	35.0	31.0	40.0	18.0	41.0
48	32.719960182055885	35.0	31.0	39.0	18.0	41.0
49	32.64579389060373	35.0	31.0	39.0	18.0	41.0
50	32.52416450127211	35.0	31.0	39.0	18.0	41.0
51	32.37734402378227	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	4.0
12	13.0
13	31.0
14	108.0
15	420.0
16	1367.0
17	3550.0
18	8294.0
19	15371.0
20	26135.0
21	40598.0
22	59249.0
23	83777.0
24	117983.0
25	171111.0
26	227250.0
27	250888.0
28	250227.0
29	257965.0
30	285353.0
31	330632.0
32	393049.0
33	487165.0
34	728006.0
35	985849.0
36	727689.0
37	883525.0
38	1188670.0
39	1692058.0
40	609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.32229208143692	22.210177987295147	26.641915167164843	19.82561476410309
2	35.06721854843522	23.699510141641213	27.4684467204444	13.764824589479167
3	26.523502894124412	24.09464085190871	36.10600035803601	13.275855895930865
4	23.736800134534743	26.218217523150283	35.05235462924286	14.992627713072112
5	21.62404049061783	30.002636446980834	34.59471951133509	13.778603551066244
6	20.502888699624062	38.395315153060395	30.922647947531452	10.179148199784095
7	79.79212212282805	4.412685324320952	12.910507271928347	2.884685280922648
8	80.19923076505786	3.8688720238256686	13.531352562398624	2.4005446487178514
9	75.21437134843956	5.511486988645919	15.513092725901737	3.761048937012786
10	42.312098904735294	23.925561058701632	21.426350365359472	12.335989671203599
11	33.08837522173821	23.70140881745046	28.315397175855356	14.894818784955977
12	28.66318033622836	20.89224743543146	32.56523036362354	17.879341864716636
13	21.576128762768594	28.062319964847372	32.026928647763086	18.334622624620945
14	18.309896440796575	29.31517475954627	33.5052593319916	18.869669467665553
15	17.595549503903136	21.93723520253446	42.31009173316553	18.157123560396876
16	20.19384937533566	20.13224548250777	39.89195992166606	19.78194522049051
17	19.6709323583181	20.373746195867394	33.308361225785106	26.646960220029403
18	22.624837934457712	21.752054638465	35.98977969935825	19.63332772771904
19	26.54342271575738	23.328074905473066	30.895307015878355	19.233195362891195
20	27.700171965780434	21.997135711922056	31.568816148509	18.73387617378851
21	24.339787022822083	24.804485214740236	32.24307390188728	18.6126538605504
22	24.77548429795106	22.292092286493904	30.695468674561543	22.236954740993493
23	21.61326686159738	26.37130504125551	30.712849695398152	21.30257840174895
24	20.337725603372046	23.486934398038397	37.6470415918498	18.528298406739758
25	19.818139406202704	24.369959693824963	34.86680517958761	20.945095720384728
26	19.53738492668399	26.305806150624665	32.710354292906004	21.446454629785343
27	19.180477272850563	26.029760387112873	33.29620970060595	21.493552639430614
28	18.84677686219411	24.446807241007058	36.25683116432226	20.449584732476577
29	19.146930383695256	23.09450523220805	35.98950845995692	21.769055924139764
30	19.945687022279603	25.862926456148728	33.42681689712975	20.764569624441926
31	24.2007388561292	24.652862389402134	30.765914971872476	20.380483782596194
32	24.733398792442184	24.018954209364267	30.50981072914576	20.737836269047786
33	24.96881289363618	24.85637873700085	28.745083785851065	21.4297245835119
34	20.405730746071097	26.43170463114154	30.60548229077949	22.557082332007877
35	21.611313937907877	26.37209706030737	30.42247164192059	21.594117359864164
36	24.68379453072871	27.182929277038497	27.609393562946526	20.523882629286263
37	20.9170495662882	29.5730691823217	28.478271011560224	21.031610239829877
38	21.992437845491185	29.194777014088174	27.410130249160513	21.402654891260124
39	21.203901507548593	28.319802103732794	28.70651354298331	21.769782845735303
40	24.39595527804751	24.677751316867294	29.115585958478675	21.81070744660652
41	20.78404461345673	24.31497404238929	29.77898328622809	25.121998057925886
42	22.34155550371869	23.9888249366656	28.185440953894727	25.48417860572098
43	22.009341484981473	24.86527538936416	29.14004090290172	23.985342222752646
44	21.479014207519842	26.365804306196733	29.199236189845884	22.95594529643754
45	19.795528889708635	29.251661341333087	27.278188554782222	23.674621214176057
46	21.124102875680133	28.188207595788196	28.3481303468067	22.339559181724976
47	21.04279615274033	27.145096805342334	29.219763587737805	22.592343454179527
48	21.683745707636472	25.788064381384295	30.783556382534353	21.744633528444876
49	22.39157204932217	25.300929266188927	30.119659974286506	22.1878387102024
50	20.896901903558117	28.756833876716264	29.086324651864224	21.259939567861387
51	20.446872338463372	27.919192357558632	27.916555910577795	23.717379393400204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6076.0
1	10283.0
2	14490.0
3	104936.0
4	195382.0
5	137194.0
6	79006.0
7	80844.5
8	82683.0
9	85563.5
10	88444.0
11	90478.5
12	92513.0
13	90791.0
14	89069.0
15	85535.0
16	82001.0
17	77220.5
18	72440.0
19	69415.5
20	66391.0
21	62816.5
22	59242.0
23	61763.5
24	64285.0
25	66174.5
26	72626.0
27	77188.0
28	92983.5
29	108779.0
30	135550.0
31	162321.0
32	175115.0
33	187909.0
34	219389.5
35	250870.0
36	271499.0
37	292128.0
38	320795.0
39	349462.0
40	407996.0
41	466530.0
42	519004.5
43	571479.0
44	590167.0
45	608855.0
46	620063.5
47	631272.0
48	632864.5
49	634457.0
50	610417.5
51	586378.0
52	545197.0
53	504016.0
54	478050.0
55	452084.0
56	435826.5
57	419569.0
58	414396.5
59	409224.0
60	390380.5
61	371537.0
62	346008.5
63	320480.0
64	288531.0
65	256582.0
66	218159.5
67	179737.0
68	147987.5
69	116238.0
70	96457.5
71	76677.0
72	63911.5
73	51146.0
74	41196.5
75	25505.0
76	19763.0
77	15433.5
78	11104.0
79	8319.5
80	5535.0
81	3937.0
82	2339.0
83	1728.0
84	1117.0
85	789.0
86	461.0
87	353.0
88	245.0
89	152.0
90	59.0
91	51.5
92	44.0
93	31.0
94	18.0
95	13.0
96	8.0
97	6.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9216950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.09644379450651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9121270652518	14.823182035248045
2	7.275066175805628	2.633056523035639
3	2.3417346544895383	1.2713120866985415
4	1.1551295470096659	0.8361494769133674
5	0.7186434116799738	0.6502445053879523
6	0.5260107858317884	0.5711354772665495
7	0.4221119591805595	0.5347107741010014
8	0.33970239547965075	0.4917924245325378
9	0.2799178514565222	0.4558965899365778
>10	2.702170847112281	10.986459133653884
>50	0.933157028344873	12.343827936088333
>100	1.3619207601932688	48.128853557713605
>500	0.028276718957119876	3.081852237216247
>1k	0.003725435631000514	1.1206496405693915
>5k	1.2214543052423076E-4	0.1802800542114539
>10k+	1.8321814578634615E-4	1.890597547426934
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125319	1.359658021362815	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	32234	0.3497252344864624	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	13511	0.14658862204959341	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0849576052815736E-5	0.0	0.0	0.036270132744563005	0.0
2	1.0849576052815736E-5	0.0	0.0	0.10623904870917168	0.0
3	2.1699152105631472E-5	0.0	0.0	0.1663673991938765	0.0
4	2.1699152105631472E-5	0.0	0.0	0.26804962596086557	0.0
5	2.1699152105631472E-5	0.0	0.0	0.4749835900162201	0.0
6	2.1699152105631472E-5	1.0849576052815736E-5	0.0	0.8331389450957204	0.0
7	2.1699152105631472E-5	1.0849576052815736E-5	0.0	1.0861836073755418	0.0
8	2.1699152105631472E-5	1.0849576052815736E-5	0.0	1.6225215499704349	0.0
9	2.1699152105631472E-5	1.0849576052815736E-5	0.0	1.916870548283326	0.0
10	2.1699152105631472E-5	1.0849576052815736E-5	0.0	2.291983790733377	0.0
11	2.1699152105631472E-5	1.0849576052815736E-5	0.0	2.5593281942508095	0.0
12	2.1699152105631472E-5	1.0849576052815736E-5	0.0	2.7760050776015928	0.0
13	4.3398304211262945E-5	1.0849576052815736E-5	0.0	2.907035407591448	0.0
14	4.3398304211262945E-5	1.0849576052815736E-5	0.0	2.9586142921465344	0.0
15	4.3398304211262945E-5	1.0849576052815736E-5	0.0	3.032467356338051	0.0
16	4.3398304211262945E-5	1.0849576052815736E-5	0.0	3.121791915980883	0.0
17	5.424788026407868E-5	1.0849576052815736E-5	0.0	3.22323545207471	0.0
18	7.594703236971015E-5	1.0849576052815736E-5	0.0	3.374044559208849	0.0
19	8.679660842252589E-5	1.0849576052815736E-5	0.0	3.450338778012249	0.0
20	9.764618447534163E-5	1.0849576052815736E-5	0.0	3.5345097890299937	0.0
21	1.0849576052815736E-4	1.0849576052815736E-5	0.0	3.634076348466684	0.0
22	1.193453365809731E-4	1.0849576052815736E-5	0.0	3.7443080411632916	0.0
23	1.3019491263378883E-4	1.0849576052815736E-5	0.0	3.8771394007779145	0.0
24	1.4104448868660458E-4	1.0849576052815736E-5	0.0	3.9831831571181358	0.0
25	1.4104448868660458E-4	1.0849576052815736E-5	0.0	4.0976895827795525	0.0
26	1.518940647394203E-4	1.0849576052815736E-5	0.0	4.188435436885304	0.0
27	1.7359321684505178E-4	1.0849576052815736E-5	0.0	4.283618767596656	0.0
28	1.8444279289786753E-4	1.0849576052815736E-5	0.0	4.376794926738238	0.0
29	1.8444279289786753E-4	1.0849576052815736E-5	0.0	4.483500507217681	0.0
30	2.1699152105631472E-4	1.0849576052815736E-5	0.0	4.625619103933514	0.0
31	2.2784109710913047E-4	1.0849576052815736E-5	0.0	4.738823580468593	0.0
32	2.386906731619462E-4	1.0849576052815736E-5	0.0	4.8741286434232585	0.0
33	2.386906731619462E-4	1.0849576052815736E-5	0.0	4.9886567682367815	0.0
34	2.495402492147619E-4	1.0849576052815736E-5	0.0	5.1318928712860545	0.0
35	2.495402492147619E-4	1.0849576052815736E-5	0.0	5.278893777225655	0.0
36	2.712394013203934E-4	1.0849576052815736E-5	0.0	5.401591632806948	0.0
37	2.8208897737320917E-4	1.0849576052815736E-5	0.0	5.537916555910578	0.0
38	3.037881294788406E-4	1.0849576052815736E-5	0.0	5.687868546536544	0.0
39	3.1463770553165636E-4	1.0849576052815736E-5	0.0	5.893326968248716	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	84610	0.0	43.263504	1
CTTGACC	6270	0.0	39.581337	7
CGTAAGG	1745	0.0	39.19771	2
TCGTAAG	590	0.0	38.898304	1
TGCATTA	5870	0.0	38.752132	15
TCAACTT	6505	0.0	38.28978	3
CATTAGA	5960	0.0	38.2802	17
GTTTTTT	100970	0.0	37.69981	2
GCATTAG	6085	0.0	37.641743	16
CTCAACT	6710	0.0	37.488823	2
GCTCAAC	6890	0.0	37.195213	1
ACTTGAC	6680	0.0	37.151947	6
GCTACGA	1315	0.0	36.958176	10
GGGCGAT	17290	0.0	36.95778	7
TAGGGAC	6270	0.0	36.854065	5
TGATTCG	990	0.0	36.81818	15
CGAATAT	1315	0.0	36.787075	14
TAGCGGG	4940	0.0	36.52834	3
TTGACCC	6540	0.0	36.467888	8
AGGGCGA	9080	0.0	36.054516	6
>>END_MODULE