FastQCFastQC Report
Thu 26 May 2016
SRR1545421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545421_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3216683
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348861.0845333531467043No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44490.13831017852862718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC6950.042.41007219
CGTTTTT207850.041.9256671
GTACCGA6400.039.37500442
TATTACG1100.038.8636361
ATAGGGC18850.038.673744
TCTACGG2050.038.4146352
TACCGAC6650.038.2330843
GGGCGAT57450.038.1462147
TATAGCG900.037.51
CGTTAGG5800.037.2413832
CACCTCG8000.037.12518
AGGGCGA29050.037.0998276
CCGACCA7200.036.87545
TGTACCG7050.036.7021341
GTAGGGC19350.036.162794
CTCGCGG8200.035.9451221
AGGGCTT24250.035.9072156
TGGGCGA21000.035.8928576
GGCGATA11850.035.8860788
GGTACCT14300.035.8741268
Produced by FastQC (version 0.11.5)