FastQCFastQC Report
Thu 26 May 2016
SRR1545420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545420_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences449307
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66281.4751606362687428No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5180.11528865564079795No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4550.10126706238718738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT351.2097189E-745.0000044
AGATACG207.0286955E-445.01
TACGCGG207.0286955E-445.02
ACTCGAA207.0286955E-445.045
ATACGAG207.0286955E-445.01
ATTGCGA207.0286955E-445.012
CGTTTTT45250.043.5082861
ACGGGTC501.0786607E-940.55
CATAGTT1300.039.8076936
CATACGA1300.039.8076918
AGGGTAT2450.039.4897966
CGGGTAT403.4519508E-739.3756
GCCGATA403.4519508E-739.3759
AGCTACG750.039.0000049
TCACGGG700.038.571433
ATGATCG700.038.5714327
CATGAGG1050.038.571432
TTGTCGC700.038.5714338
ACGAATG356.2397958E-638.571431
GGCGATA2050.038.4146358
Produced by FastQC (version 0.11.5)