##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545420_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	449307
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211325441179415	31.0	31.0	33.0	30.0	34.0
2	31.58125958420412	31.0	31.0	34.0	30.0	34.0
3	31.5903380094234	31.0	31.0	34.0	30.0	34.0
4	35.38327023616369	37.0	35.0	37.0	33.0	37.0
5	35.28453596316105	37.0	35.0	37.0	33.0	37.0
6	35.3476820970962	37.0	35.0	37.0	33.0	37.0
7	35.787673016445325	37.0	35.0	37.0	35.0	37.0
8	35.796693574771815	37.0	35.0	37.0	35.0	37.0
9	37.44360537449895	39.0	37.0	39.0	35.0	39.0
10	36.861902885999996	39.0	37.0	39.0	32.0	39.0
11	36.55658825702693	39.0	35.0	39.0	32.0	39.0
12	36.19413897401999	38.0	35.0	39.0	32.0	39.0
13	36.042196093094475	38.0	35.0	39.0	31.0	39.0
14	37.12791921781766	39.0	35.0	41.0	31.0	41.0
15	37.223887008214874	39.0	35.0	41.0	32.0	41.0
16	37.28839523978037	39.0	35.0	41.0	32.0	41.0
17	37.21091592163042	39.0	35.0	41.0	32.0	41.0
18	36.99951258271071	39.0	35.0	40.0	31.0	41.0
19	36.83051232230969	38.0	35.0	40.0	31.0	41.0
20	36.61827659039365	38.0	35.0	40.0	31.0	41.0
21	36.449888383666405	38.0	35.0	40.0	31.0	41.0
22	36.412882505725484	38.0	35.0	40.0	31.0	41.0
23	36.29780751245807	38.0	35.0	40.0	30.0	41.0
24	36.185067225749876	38.0	35.0	40.0	30.0	41.0
25	36.059829915848184	38.0	35.0	40.0	30.0	41.0
26	35.96268253109789	38.0	35.0	40.0	30.0	41.0
27	35.906569450286774	38.0	35.0	40.0	30.0	41.0
28	35.82212829980392	38.0	35.0	40.0	30.0	41.0
29	35.736859207624185	37.0	34.0	40.0	29.0	41.0
30	35.585341425795725	37.0	34.0	40.0	29.0	41.0
31	35.359315568197246	37.0	34.0	40.0	27.0	41.0
32	35.040945277950264	37.0	34.0	40.0	25.0	41.0
33	34.65908832936055	37.0	33.0	40.0	24.0	41.0
34	34.25582953303643	37.0	33.0	40.0	22.0	41.0
35	33.95191261208928	37.0	33.0	40.0	20.0	41.0
36	33.76509157435785	37.0	33.0	40.0	19.0	41.0
37	33.66943760057155	37.0	33.0	40.0	18.0	41.0
38	33.60519644697278	37.0	33.0	40.0	18.0	41.0
39	33.47901991288807	36.0	33.0	40.0	18.0	41.0
40	33.345498734718134	36.0	32.0	40.0	18.0	41.0
41	33.23288531004413	36.0	32.0	40.0	18.0	41.0
42	33.09813112192777	36.0	32.0	40.0	17.0	41.0
43	32.99754733400548	36.0	32.0	40.0	16.0	41.0
44	32.88111692005689	35.0	31.0	40.0	15.0	41.0
45	32.857858880453676	35.0	31.0	40.0	17.0	41.0
46	32.80308118947624	35.0	31.0	40.0	17.0	41.0
47	32.72996637043269	35.0	31.0	40.0	17.0	41.0
48	32.66160553919703	35.0	31.0	40.0	17.0	41.0
49	32.57448248079821	35.0	31.0	39.0	15.0	41.0
50	32.46249001239687	35.0	31.0	39.0	15.0	41.0
51	32.30745792965612	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	21.0
16	71.0
17	181.0
18	372.0
19	750.0
20	1240.0
21	1932.0
22	2944.0
23	4158.0
24	5960.0
25	8430.0
26	11362.0
27	12514.0
28	12506.0
29	12607.0
30	13662.0
31	15696.0
32	18561.0
33	22847.0
34	34044.0
35	44898.0
36	36323.0
37	43802.0
38	59018.0
39	85375.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.646290843454477	21.706761746422824	25.804405451061303	20.8425419590614
2	37.50353321893493	23.234447716149536	26.630789193135207	12.63122987178032
3	26.95195044813457	23.620152813109968	36.111389317326456	13.316507421429
4	24.131161989463774	25.920584366591214	35.564547180435646	14.38370646350936
5	21.71165817581297	29.63875479349309	34.51604359602677	14.133543434667164
6	20.303267031228092	37.64797788594436	32.128366573411945	9.920388509415611
7	79.3510895668218	4.386087908712751	14.037395366642407	2.225427157823048
8	79.46682335240716	4.0549112299608066	14.238816666555385	2.239448751076658
9	74.45710839136716	5.803159087216535	16.076758207639767	3.6629743137765494
10	39.98557778979628	24.055712463861013	22.97048565902601	12.988224087316691
11	31.136839621906624	23.74234098289143	29.287102137291427	15.833717257910514
12	27.772770065901486	21.398064129871113	34.19109873649865	16.638067067728745
13	21.72812798376166	27.2061196464778	33.92023716523446	17.14551520452608
14	18.65984727591602	28.76385188746225	35.252511089299745	17.32378974732199
15	17.975682551128738	22.65355313849996	41.866696935502894	17.50406737486841
16	19.87571971947911	21.12809281849604	39.45075416140857	19.54543330061628
17	20.201777403868622	21.35622191508256	34.226041437146534	24.215959243902276
18	21.711213045868416	22.31236103599543	36.422980278517805	19.553445639618346
19	25.501049393844298	23.563621310150964	31.4426438938187	19.492685402186034
20	26.85758289988805	22.441226154945284	32.127253748550544	18.57393719661612
21	23.45278395395576	24.502845493170593	32.648946043573766	19.39542450929988
22	23.238676450622847	23.04326440496142	31.865739906122094	21.85231923829364
23	21.328401293547618	25.033662952057277	32.15195846047357	21.48597729392153
24	20.542746941400868	22.52824905910658	37.682698021619956	19.24630597787259
25	20.838313224588088	24.293411854255552	34.5874869521285	20.28078796902786
26	19.71881141402204	26.69555560006855	32.69501699283563	20.890615993073776
27	19.58594012557116	26.243526141368818	33.07738361521187	21.093150117848154
28	19.024631265482174	25.224401133300837	35.891272559742006	19.859695041474982
29	19.88039358389698	23.227103072064313	35.319058016011326	21.573445328027386
30	20.445486048514713	25.411578274987928	34.1274451544267	20.015490522070657
31	23.954000271529267	25.08574315557069	31.487602018219167	19.472654554680876
32	25.088413935238048	24.13338763918657	30.665001880674016	20.11319654490137
33	24.529998419788697	25.546007518244764	29.515676363822507	20.408317698144028
34	21.27498570020053	26.162067361514513	30.955449169498806	21.607497768786153
35	22.01924296750329	26.517503622244927	30.746683225500604	20.716570184751184
36	24.687574420162605	27.33031090100978	27.723138077083153	20.258976601744465
37	22.031929170923224	29.276641583594294	29.193847413906305	19.497581831576184
38	22.06709443654339	28.63565446342924	28.39239094872771	20.90486015129967
39	21.82672426648149	29.232128589138384	28.216119490682317	20.725027653697804
40	23.93374685905183	26.512607192854777	28.55864698302052	20.994998965072877
41	20.531396127814613	26.194116717522764	29.474056714006235	23.80043044065639
42	21.83228839078848	27.040753872074102	27.931904021081355	23.195053716056062
43	21.944461136817363	26.294938649965395	29.273080544037818	22.487519669179427
44	21.61818088745557	27.460511409793302	29.252827131560384	21.668480571190745
45	20.240503709045264	29.88780499747389	27.975526755648144	21.896164537832707
46	21.191301270623427	29.0634243401505	28.29468492589699	21.45058946332908
47	21.45281511305188	27.558662562568575	29.452913041639682	21.535609282739866
48	21.726124899011143	26.150048853011416	30.53836241144696	21.58546383653048
49	21.407189293734575	26.43426432261238	30.3019984108861	21.856547972766947
50	20.135675607101604	29.12329431769369	29.81012982214833	20.93090025305637
51	20.315730669675744	28.65034375159969	28.98040760549023	22.053517973234335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	566.5
2	832.0
3	5706.5
4	10581.0
5	7546.0
6	4511.0
7	4681.5
8	4852.0
9	4980.0
10	5108.0
11	5113.5
12	5119.0
13	5084.0
14	5049.0
15	4757.0
16	4465.0
17	4198.5
18	3932.0
19	3791.0
20	3650.0
21	3506.0
22	3362.0
23	3410.5
24	3459.0
25	3809.0
26	4361.0
27	4563.0
28	4920.5
29	5278.0
30	6203.5
31	7129.0
32	8327.0
33	9525.0
34	10944.0
35	12363.0
36	13708.0
37	15053.0
38	16279.0
39	17505.0
40	20059.5
41	22614.0
42	24660.5
43	26707.0
44	27628.0
45	28549.0
46	28417.0
47	28285.0
48	28169.5
49	28054.0
50	28102.0
51	28150.0
52	26746.0
53	25342.0
54	24015.0
55	22688.0
56	21963.0
57	21238.0
58	19999.0
59	18760.0
60	17780.0
61	16800.0
62	15565.5
63	14331.0
64	12980.5
65	11630.0
66	10054.0
67	8478.0
68	7248.0
69	6018.0
70	5121.0
71	4224.0
72	3600.5
73	2977.0
74	2299.0
75	1296.5
76	972.0
77	798.0
78	624.0
79	410.5
80	197.0
81	164.5
82	132.0
83	107.0
84	82.0
85	49.5
86	17.0
87	13.0
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.54799096446399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.50490429448838	26.407264317654082
2	8.38479129134183	6.464337178751226
3	4.812609044176938	5.56549229851291
4	3.4566690020117563	5.329905818267678
5	2.6946724186510442	5.19371040231754
6	2.166519547213693	5.010898555819684
7	1.8996262811650577	5.125874370554629
8	1.4453814226433863	4.457324001620909
9	1.22284939292844	4.2424548614555215
>10	5.386381851911474	29.647530364088322
>50	0.01745144554632914	0.45453324031161724
>100	0.006980578218531605	0.4982599210520018
>500	5.817148515443003E-4	0.11615600319754137
>1k	0.0	0.0
>5k	5.817148515443003E-4	1.4862586663963402
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6628	1.4751606362687428	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	518	0.11528865564079795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	455	0.10126706238718738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021588802311114673	0.0
2	0.0	0.0	0.0	0.06276332218282822	0.0
3	0.0	0.0	0.0	0.11083735619520729	0.0
4	0.0	0.0	0.0	0.17849710776818523	0.0
5	0.0	0.0	0.0	0.3374084979757716	0.0
6	0.0	0.0	0.0	0.6423225099987314	0.0
7	0.0	0.0	0.0	0.8366217307987635	0.0
8	0.0	0.0	0.0	1.188274387000425	0.0
9	0.0	0.0	0.0	1.3825736078004571	0.0
10	0.0	0.0	0.0	1.6385233259219198	0.0
11	2.225649722795327E-4	0.0	0.0	1.8034439703810534	0.0
12	2.225649722795327E-4	0.0	0.0	1.9418793831389227	0.0
13	2.225649722795327E-4	0.0	0.0	2.015103259018889	0.0
14	2.225649722795327E-4	0.0	0.0	2.0565003438628824	0.0
15	2.225649722795327E-4	0.0	0.0	2.0950040840672415	0.0
16	2.225649722795327E-4	0.0	0.0	2.1522032819430814	0.0
17	2.225649722795327E-4	0.0	0.0	2.2163019939595867	0.0
18	2.225649722795327E-4	0.0	0.0	2.301989508287207	0.0
19	2.225649722795327E-4	0.0	0.0	2.3478378925767904	0.0
20	2.225649722795327E-4	0.0	0.0	2.4039242655912325	0.0
21	2.225649722795327E-4	0.0	0.0	2.4693583674414152	0.0
22	2.225649722795327E-4	0.0	0.0	2.5434725032105	0.0
23	2.225649722795327E-4	0.0	0.0	2.6198122887023794	0.0
24	2.225649722795327E-4	0.0	0.0	2.685246390552562	0.0
25	2.225649722795327E-4	0.0	0.0	2.747119452846272	0.0
26	2.225649722795327E-4	0.0	0.0	2.8052089106112303	0.0
27	2.225649722795327E-4	0.0	0.0	2.8713107073782513	0.0
28	2.225649722795327E-4	0.0	0.0	2.9318483798382844	0.0
29	2.225649722795327E-4	0.0	0.0	3.006185080579648	0.0
30	2.225649722795327E-4	0.0	0.0	3.0981044141310954	0.0
31	2.225649722795327E-4	0.0	0.0	3.1800083239299632	0.0
32	2.225649722795327E-4	0.0	0.0	3.2594640190337563	0.0
33	2.225649722795327E-4	0.0	0.0	3.339364844082109	0.0
34	4.451299445590654E-4	0.0	0.0	3.4408544714415754	0.0
35	4.451299445590654E-4	0.0	0.0	3.5327738049930226	0.0
36	4.451299445590654E-4	0.0	0.0	3.621799793904836	0.0
37	4.451299445590654E-4	0.0	0.0	3.711716042705767	0.0
38	4.451299445590654E-4	0.0	0.0	3.8094220655364817	0.0
39	4.451299445590654E-4	0.0	0.0	3.9429610489042015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	35	1.2097189E-7	45.000004	4
AGATACG	20	7.0286955E-4	45.0	1
TACGCGG	20	7.0286955E-4	45.0	2
ACTCGAA	20	7.0286955E-4	45.0	45
ATACGAG	20	7.0286955E-4	45.0	1
ATTGCGA	20	7.0286955E-4	45.0	12
CGTTTTT	4525	0.0	43.508286	1
ACGGGTC	50	1.0786607E-9	40.5	5
CATAGTT	130	0.0	39.80769	36
CATACGA	130	0.0	39.80769	18
AGGGTAT	245	0.0	39.489796	6
CGGGTAT	40	3.4519508E-7	39.375	6
GCCGATA	40	3.4519508E-7	39.375	9
AGCTACG	75	0.0	39.000004	9
TCACGGG	70	0.0	38.57143	3
ATGATCG	70	0.0	38.57143	27
CATGAGG	105	0.0	38.57143	2
TTGTCGC	70	0.0	38.57143	38
ACGAATG	35	6.2397958E-6	38.57143	1
GGCGATA	205	0.0	38.414635	8
>>END_MODULE