FastQCFastQC Report
Thu 26 May 2016
SRR1545419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545419_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4552872
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473241.0394318135893124No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC68030.14942216693111512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT365100.042.578061
CGTAAGG5700.038.2894742
AGGGCGA43850.038.17566
GGGCGAT84150.037.486637
TATAGCG2050.037.3170741
ACGGGTA6100.037.25415
TAGGGCG17500.036.6428575
GTTTTTT455500.036.004942
GACCGAT26600.035.9492459
ATAGCGG7650.035.882352
CTACGGG8050.035.7763983
TCGTTGA13050.035.68965524
GCGTAAG2650.035.6603781
GCCCTAC28900.035.6574426
CCCTAGC28900.035.5017321
ATTATAC28750.035.4521742
TACGGGA9850.035.406094
TAGGGAT39200.035.3571435
CGGTCTA2050.035.12195231
CGAATAT7500.035.10000214
Produced by FastQC (version 0.11.5)