##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545419_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4552872
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276672614560656	31.0	31.0	33.0	30.0	34.0
2	31.64381537631631	31.0	31.0	34.0	30.0	34.0
3	31.682408378711283	31.0	31.0	34.0	30.0	34.0
4	35.44796932573549	37.0	35.0	37.0	33.0	37.0
5	35.3418255553857	37.0	35.0	37.0	33.0	37.0
6	35.40600548401097	37.0	35.0	37.0	33.0	37.0
7	35.83075188584261	37.0	35.0	37.0	35.0	37.0
8	35.849761205674135	37.0	35.0	37.0	35.0	37.0
9	37.509938122574056	39.0	37.0	39.0	35.0	39.0
10	36.910242150449214	39.0	37.0	39.0	32.0	39.0
11	36.5873215851445	39.0	35.0	39.0	32.0	39.0
12	36.127173353434934	38.0	35.0	39.0	32.0	39.0
13	35.91895049981638	38.0	35.0	39.0	31.0	39.0
14	36.96486195965975	39.0	35.0	41.0	31.0	41.0
15	37.10404443612735	39.0	35.0	41.0	32.0	41.0
16	37.200336403044055	39.0	35.0	41.0	32.0	41.0
17	37.13854903015064	39.0	35.0	41.0	32.0	41.0
18	36.96905952989673	39.0	35.0	40.0	32.0	41.0
19	36.84174933975741	38.0	35.0	40.0	31.0	41.0
20	36.63592936502498	38.0	35.0	40.0	31.0	41.0
21	36.456828568868175	38.0	35.0	40.0	31.0	41.0
22	36.41527919080528	38.0	35.0	40.0	31.0	41.0
23	36.33866710946409	38.0	35.0	40.0	31.0	41.0
24	36.23245349309183	38.0	35.0	40.0	30.0	41.0
25	36.10648750942262	38.0	35.0	40.0	30.0	41.0
26	36.0176785114978	38.0	35.0	40.0	30.0	41.0
27	35.96865429118148	38.0	35.0	40.0	30.0	41.0
28	35.913531722394126	38.0	35.0	40.0	30.0	41.0
29	35.857149509144996	38.0	35.0	40.0	30.0	41.0
30	35.69956546109796	37.0	34.0	40.0	29.0	41.0
31	35.48197533337199	37.0	34.0	40.0	29.0	41.0
32	35.188890880305884	37.0	34.0	40.0	27.0	41.0
33	34.85550395442701	37.0	34.0	40.0	25.0	41.0
34	34.51594136624092	37.0	33.0	40.0	23.0	41.0
35	34.273372280178314	37.0	33.0	40.0	23.0	41.0
36	34.097304294959315	37.0	33.0	40.0	21.0	41.0
37	34.02060633376032	37.0	33.0	40.0	21.0	41.0
38	33.95048026827901	37.0	33.0	40.0	21.0	41.0
39	33.84454581635504	36.0	33.0	40.0	21.0	41.0
40	33.69702332945007	36.0	33.0	40.0	20.0	41.0
41	33.616687664401724	36.0	33.0	40.0	19.0	41.0
42	33.51505049999209	36.0	33.0	40.0	18.0	41.0
43	33.41626208687615	36.0	32.0	40.0	18.0	41.0
44	33.32821304881842	36.0	32.0	40.0	18.0	41.0
45	33.30677888594276	35.0	32.0	40.0	18.0	41.0
46	33.25918628944543	35.0	32.0	40.0	18.0	41.0
47	33.19465251823464	35.0	32.0	40.0	18.0	41.0
48	33.13551182638124	35.0	32.0	40.0	18.0	41.0
49	33.05234673849825	35.0	32.0	40.0	18.0	41.0
50	32.9430682435175	35.0	32.0	39.0	18.0	41.0
51	32.789668587212645	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	11.0
13	26.0
14	54.0
15	198.0
16	587.0
17	1621.0
18	3584.0
19	7056.0
20	11786.0
21	18359.0
22	27678.0
23	39200.0
24	55462.0
25	80256.0
26	103496.0
27	113093.0
28	112756.0
29	118333.0
30	131422.0
31	155107.0
32	186306.0
33	234579.0
34	361690.0
35	506515.0
36	348191.0
37	428789.0
38	592233.0
39	914172.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.59970849169491	22.387692867271472	26.53287858740593	18.479720053627688
2	35.76603954602721	24.107332690222787	27.102958308513834	13.023669455236167
3	27.606486630856303	24.28570361740897	34.71211138815236	13.395698363582373
4	24.706317243269744	26.522972752144142	33.92388364970507	14.84682635488105
5	22.080480189208043	30.363888991388293	33.528177379025806	14.027453440377855
6	21.255286772832623	37.81384585378196	31.131053102305533	9.79981427107988
7	81.26048788544901	4.75664591493018	12.025486330386622	1.9573798692341888
8	81.87882286170137	3.9270377027950705	12.091774159255959	2.102365276247608
9	76.94793528129058	5.620144822872245	14.022225970771856	3.409693925065321
10	42.50018449892727	23.507711176593588	21.628435853237253	12.363668471241889
11	34.54641378013702	23.89920032893523	27.038273863179107	14.51611202774864
12	31.00757060598233	21.807092314477543	31.187720629967192	15.997616449572929
13	21.954647527977944	30.590888564405063	31.13902609166258	16.31543781595441
14	18.437153515407417	31.53134988200854	33.041517530033786	16.98997907255025
15	17.521577588827448	23.56462030999334	42.486610649278084	16.42719145190113
16	19.551439179489343	21.12470985347271	40.47412270760083	18.84972825943712
17	20.114600190824603	21.332051505071963	33.085819236736725	25.467529067366705
18	22.08041429673402	22.63492142981397	36.00507547763258	19.27958879581943
19	26.64711417320759	23.571802589662084	30.560753739617542	19.22032949751278
20	27.66594360658503	22.838287568813705	31.327149105004487	18.168619719596773
21	23.4030958919996	25.51993554837474	32.29251777778949	18.784450781836167
22	23.586584468001735	23.40035037224855	30.743627319195443	22.26943784055427
23	21.528850360827185	26.51734992769399	30.57786381870608	21.375935892772738
24	20.66120022702154	23.278888578462123	37.956722701626575	18.103188492889764
25	19.930474654240225	24.71993502123495	35.19877123714438	20.15081908738045
26	19.316620366221585	28.720530689200135	31.52414563818179	20.43870330639649
27	18.927547271260867	29.031345489176942	32.58808945210847	19.45301778745372
28	18.4025819307022	26.346710384126766	36.28026441331977	18.970443271851263
29	19.276052566380077	23.93300316811015	36.06668494084613	20.72425932466364
30	20.261716121164838	27.618149598758762	33.17637746020534	18.943756819871062
31	24.71073203902943	26.13925891173747	30.132167124399718	19.017841924833377
32	26.092233649441493	24.481953369213983	30.26726866030936	19.158544321035162
33	25.377871374376436	26.441903044935156	28.54415850039272	19.636067080295692
34	20.68105582586113	26.342207731735044	30.75201762755465	22.224718814849176
35	21.527532511346685	26.425407962270846	31.173927138737923	20.873132387644546
36	26.1526790122806	26.67795185105138	27.50973451482932	19.6596346218387
37	21.55276932889833	30.091379682978133	29.249383685726283	19.106467302397256
38	21.687585330753862	29.79868531335825	27.987279238247858	20.52645011764003
39	21.457291134035835	30.054853288210165	28.475256936720385	20.012598641033616
40	24.32082430606439	26.570151763546175	27.961317603481934	21.1477063269075
41	19.997333551217782	25.206572906068956	29.742193498960656	25.053900043752602
42	21.43108789353182	26.158257908414733	28.118580974822045	24.2920732232314
43	21.549584525987115	26.53582178457905	28.870875350767605	23.04371833866623
44	20.710070478590218	28.244018281207993	29.476449151217075	21.56946208898471
45	19.320749627927164	31.949283880592294	26.86032025499509	21.869646236485455
46	21.333303462078444	30.073676571623366	27.91097575332669	20.682044212971505
47	21.029517192664322	27.746464209843808	29.473989165520138	21.750029431971733
48	21.66034977482345	26.321166068363	31.142540356943925	20.87594379986962
49	21.351467820751385	26.30216707168574	30.933287823597936	21.413077283964935
50	20.201666113169885	29.889199608510847	29.295244847647815	20.613889430671453
51	19.74628322518182	30.569737080242977	27.850003250695387	21.83397644387982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2781.0
1	5231.5
2	7682.0
3	43761.0
4	79840.0
5	57673.0
6	35506.0
7	35915.0
8	36324.0
9	37971.5
10	39619.0
11	40173.5
12	40728.0
13	40989.5
14	41251.0
15	39885.5
16	38520.0
17	36636.5
18	34753.0
19	33469.5
20	32186.0
21	33091.0
22	33996.0
23	34365.0
24	34734.0
25	36388.5
26	42905.5
27	47768.0
28	57243.0
29	66718.0
30	77312.5
31	87907.0
32	98229.0
33	108551.0
34	127916.5
35	147282.0
36	153141.5
37	159001.0
38	176482.0
39	193963.0
40	230893.5
41	267824.0
42	296446.5
43	325069.0
44	334892.0
45	344715.0
46	328552.0
47	312389.0
48	305613.0
49	298837.0
50	288606.0
51	278375.0
52	267064.0
53	255753.0
54	239995.0
55	224237.0
56	204186.5
57	184136.0
58	175134.0
59	166132.0
60	154466.5
61	142801.0
62	131543.5
63	120286.0
64	110799.5
65	101313.0
66	88157.5
67	75002.0
68	63937.0
69	52872.0
70	45235.0
71	37598.0
72	30601.5
73	23605.0
74	19596.5
75	12290.5
76	8993.0
77	7258.0
78	5523.0
79	4099.0
80	2675.0
81	1970.0
82	1265.0
83	836.5
84	408.0
85	301.5
86	195.0
87	128.5
88	62.0
89	44.0
90	26.0
91	19.5
92	13.0
93	9.5
94	6.0
95	8.0
96	10.0
97	9.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4552872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.44319923694688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74032069387594	15.07553020249086
2	5.832207620125214	2.151291342584183
3	2.0833637033754853	1.1527167559313274
4	1.1883225159906683	0.8766587568066357
5	0.8276676928495295	0.7632420080604014
6	0.6120166971301566	0.6772527528905794
7	0.49497422137147146	0.6390235727334689
8	0.38911974290004536	0.5741290356268067
9	0.3461619564998891	0.5745900538780916
>10	3.606547502076294	15.892921555449155
>50	1.5143040432278412	20.346433535037484
>100	1.3602749637645095	39.32040260818934
>500	0.0033877464297382633	0.3895459869631231
>1k	0.001088918495272988	0.35843943338423134
>5k	1.2099094391922088E-4	0.15180622955319215
>10k+	1.2099094391922088E-4	1.0560161704211768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47324	1.0394318135893124	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6803	0.14942216693111512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010345118421954317	0.0
2	0.0	0.0	0.0	0.03432997896712229	0.0
3	0.0	0.0	0.0	0.06167535568757479	0.0
4	0.0	0.0	0.0	0.09897049598583048	0.0
5	0.0	0.0	0.0	0.17788771570999581	0.0
6	0.0	0.0	0.0	0.3435413954093153	0.0
7	2.196415800839558E-5	0.0	0.0	0.4457845509383967	0.0
8	2.196415800839558E-5	0.0	0.0	0.6572774284012377	0.0
9	4.392831601679116E-5	0.0	0.0	0.7848013297979824	0.0
10	8.785663203358232E-5	0.0	0.0	0.9401098910753476	0.0
11	8.785663203358232E-5	0.0	0.0	1.055729218831542	0.0
12	1.098207900419779E-4	0.0	0.0	1.1439812057092753	0.0
13	1.3178494805037347E-4	0.0	0.0	1.1921266400636785	0.0
14	1.3178494805037347E-4	0.0	0.0	1.2196257658901897	0.0
15	1.3178494805037347E-4	0.0	0.0	1.2437863396994249	0.0
16	1.3178494805037347E-4	0.0	0.0	1.2844639603309735	0.0
17	1.3178494805037347E-4	0.0	0.0	1.3298419107763189	0.0
18	1.3178494805037347E-4	0.0	0.0	1.3896503130331799	0.0
19	1.3178494805037347E-4	0.0	0.0	1.426132779485125	0.0
20	1.3178494805037347E-4	0.0	0.0	1.4656023714262119	0.0
21	1.3178494805037347E-4	0.0	0.0	1.5143847663628585	0.0
22	1.3178494805037347E-4	0.0	0.0	1.570722831654393	0.0
23	1.3178494805037347E-4	0.0	0.0	1.6310583737034559	0.0
24	1.3178494805037347E-4	0.0	0.0	1.681268438910648	0.0
25	1.3178494805037347E-4	0.0	0.0	1.7270637083581528	0.0
26	1.3178494805037347E-4	0.0	0.0	1.7740670064961195	0.0
27	1.3178494805037347E-4	0.0	0.0	1.823288684592934	0.0
28	1.5374910605876906E-4	0.0	0.0	1.8710387641031858	0.0
29	1.5374910605876906E-4	0.0	0.0	1.9259052308081581	0.0
30	1.7571326406716465E-4	0.0	0.0	2.002977461259618	0.0
31	1.976774220755602E-4	0.0	0.0	2.0647846018952434	0.0
32	1.976774220755602E-4	0.0	0.0	2.127448344693196	0.0
33	2.196415800839558E-4	0.0	0.0	2.1916056502357195	0.0
34	2.196415800839558E-4	0.0	0.0	2.2609245329102157	0.0
35	2.196415800839558E-4	0.0	0.0	2.345398684610505	0.0
36	2.196415800839558E-4	0.0	0.0	2.41350953859454	0.0
37	2.4160573809235139E-4	0.0	0.0	2.4900985575698154	0.0
38	2.4160573809235139E-4	0.0	0.0	2.5738478920558276	0.0
39	3.074982121175381E-4	0.0	0.0	2.7069726537447134	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	36510	0.0	42.57806	1
CGTAAGG	570	0.0	38.289474	2
AGGGCGA	4385	0.0	38.1756	6
GGGCGAT	8415	0.0	37.48663	7
TATAGCG	205	0.0	37.317074	1
ACGGGTA	610	0.0	37.2541	5
TAGGGCG	1750	0.0	36.642857	5
GTTTTTT	45550	0.0	36.00494	2
GACCGAT	2660	0.0	35.949245	9
ATAGCGG	765	0.0	35.88235	2
CTACGGG	805	0.0	35.776398	3
TCGTTGA	1305	0.0	35.689655	24
GCGTAAG	265	0.0	35.660378	1
GCCCTAC	2890	0.0	35.65744	26
CCCTAGC	2890	0.0	35.50173	21
ATTATAC	2875	0.0	35.45217	42
TACGGGA	985	0.0	35.40609	4
TAGGGAT	3920	0.0	35.357143	5
CGGTCTA	205	0.0	35.121952	31
CGAATAT	750	0.0	35.100002	14
>>END_MODULE