FastQCFastQC Report
Thu 26 May 2016
SRR1545418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545418_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1770351
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151860.8577959963871571No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20340.11489247047619372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA207.034321E-445.018
CGTTTTT112400.041.8171731
ATAACGG1350.038.3333322
TATACGC301.1401711E-437.49999644
ATAGGGC9050.037.2928164
CGTTCGG4000.037.12545
TATGACG1100.036.818181
AGGGCGA21550.036.5429236
GGGCGAT39850.036.530747
ACGGGAT4250.036.529415
CGCGACC3450.036.5217410
CGACCCG3100.036.2903232
CGTACAC250.002107415636.037
GTTTTTT141950.035.6481172
TACGGGT1650.035.4545444
ATGACGG2750.035.181822
TACGGGA3550.034.8591584
CTAAGCG1100.034.7727281
GGCGATA8800.034.7727288
AATACGA653.6743586E-1034.61538327
Produced by FastQC (version 0.11.5)