##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545418_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1770351
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246938601441183	31.0	31.0	33.0	30.0	34.0
2	31.61143637617625	31.0	31.0	34.0	30.0	34.0
3	31.67271461986917	31.0	31.0	34.0	30.0	34.0
4	35.42935214542201	37.0	35.0	37.0	33.0	37.0
5	35.30724867554513	37.0	35.0	37.0	33.0	37.0
6	35.371864110563386	37.0	35.0	37.0	33.0	37.0
7	35.794354904761825	37.0	35.0	37.0	35.0	37.0
8	35.80943270571768	37.0	35.0	37.0	35.0	37.0
9	37.45392862771281	39.0	37.0	39.0	35.0	39.0
10	36.854337925078134	39.0	37.0	39.0	32.0	39.0
11	36.54961078339832	39.0	35.0	39.0	32.0	39.0
12	36.12154877761529	38.0	35.0	39.0	31.0	39.0
13	35.93312060715643	38.0	35.0	39.0	31.0	39.0
14	36.997614032471525	39.0	35.0	41.0	31.0	41.0
15	37.09450442313417	39.0	35.0	41.0	31.0	41.0
16	37.16003549578586	39.0	35.0	41.0	32.0	41.0
17	37.09553868131235	39.0	35.0	41.0	32.0	41.0
18	36.94102920833213	38.0	35.0	40.0	31.0	41.0
19	36.858585105439545	38.0	35.0	40.0	31.0	41.0
20	36.712192102018186	38.0	35.0	40.0	31.0	41.0
21	36.532086010062415	38.0	35.0	40.0	31.0	41.0
22	36.462635375696685	38.0	35.0	40.0	31.0	41.0
23	36.364834431138235	38.0	35.0	40.0	30.0	41.0
24	36.28507906059307	38.0	35.0	40.0	30.0	41.0
25	36.158350519190826	38.0	35.0	40.0	30.0	41.0
26	36.07111810030892	38.0	35.0	40.0	30.0	41.0
27	36.032412781420184	38.0	35.0	40.0	30.0	41.0
28	35.950640861614445	38.0	35.0	40.0	30.0	41.0
29	35.890220639861816	38.0	35.0	40.0	30.0	41.0
30	35.760768909668194	38.0	34.0	40.0	29.0	41.0
31	35.57719909780603	38.0	34.0	40.0	29.0	41.0
32	35.361992621802116	38.0	34.0	40.0	27.0	41.0
33	35.09549631683208	38.0	34.0	40.0	26.0	41.0
34	34.807543814757636	37.0	34.0	40.0	24.0	41.0
35	34.607791901154066	37.0	33.0	40.0	24.0	41.0
36	34.46191461467246	37.0	33.0	40.0	23.0	41.0
37	34.402007285560884	37.0	33.0	40.0	23.0	41.0
38	34.32891443561192	37.0	33.0	40.0	23.0	41.0
39	34.21892325307241	37.0	33.0	40.0	23.0	41.0
40	34.06329083893533	37.0	33.0	40.0	23.0	41.0
41	33.9575095560146	36.0	33.0	40.0	22.0	41.0
42	33.8478940052001	36.0	33.0	40.0	22.0	41.0
43	33.76699027480991	36.0	33.0	40.0	22.0	41.0
44	33.68922546997742	36.0	33.0	40.0	21.0	41.0
45	33.647164884251765	36.0	33.0	40.0	22.0	41.0
46	33.606531134221406	36.0	33.0	40.0	22.0	41.0
47	33.53418672342377	35.0	32.0	40.0	21.0	41.0
48	33.45503857709573	35.0	32.0	40.0	21.0	41.0
49	33.37184547019207	35.0	32.0	40.0	20.0	41.0
50	33.249407603350974	35.0	32.0	39.0	20.0	41.0
51	33.0836811457163	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	9.0
14	20.0
15	64.0
16	213.0
17	548.0
18	1191.0
19	2316.0
20	3970.0
21	6230.0
22	9334.0
23	13062.0
24	18669.0
25	26594.0
26	35718.0
27	40696.0
28	43308.0
29	46137.0
30	52335.0
31	62033.0
32	74306.0
33	93618.0
34	140074.0
35	191180.0
36	143199.0
37	175719.0
38	239837.0
39	349849.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.82942196208548	22.555188208440022	27.412812487467175	17.20257734200732
2	34.96402690765842	24.27942255518821	27.84809340068721	12.90845713646616
3	28.627373893651598	24.49502951674555	33.02249102014233	13.855105569460518
4	25.851653146748866	26.820161651559495	32.5837079765538	14.744477225137839
5	22.758311769812877	30.734244226144984	31.799456717905095	14.707987286137042
6	22.24163456851212	38.221234094255884	29.42320477690582	10.113926560326172
7	82.72421683609635	4.720419848945209	10.297675432724922	2.2576878822335233
8	83.41249842545349	3.7649031180822337	10.431773134254167	2.3908253222101155
9	77.8995803657015	5.80048815178459	12.296262153663314	4.003669328850607
10	42.23388469292247	24.18938391313361	19.56233537869044	14.014396015253471
11	34.643525492967214	24.290154890188443	25.5395116561631	15.526807960681243
12	31.074572217599787	21.964344923690273	29.904408786732123	17.056674071977817
13	22.824569816945907	28.08313153719234	30.72091353635522	18.371385109506534
14	19.98982122754188	30.217510538870542	31.925420439223632	17.86724779436394
15	19.60650741011246	22.860155980367736	40.18039360556184	17.35294300395797
16	21.42642899628379	21.051475102959806	38.48530602123534	19.036789879521066
17	21.555160530312914	21.084971285355277	31.434387869976067	25.92548031435574
18	23.664064357858976	22.6728484916268	33.44528853317789	20.217798617336335
19	27.722242651316037	23.497374249513232	28.866930908051568	19.913452191119163
20	29.675132219542906	22.608341509678024	28.622290156019908	19.094236114759163
21	25.321023909947804	25.170714733970833	29.847640383178252	19.660620972903114
22	24.600827745458385	23.3154894142461	29.409309227379204	22.67437361291631
23	23.02820175208193	25.654178182744552	29.24855014627043	22.069069918903086
24	22.486557750412207	23.24222710637608	34.54292397383344	19.728291169378277
25	21.86781039466185	24.09465693526312	32.72085592066206	21.31667674941297
26	20.36070813076051	28.431311078989417	29.845606888125577	21.362373902124492
27	20.605913742529026	27.72207319339498	30.9456712256496	20.72634183842639
28	20.422164870130274	26.05618885746386	33.68168233305147	19.839963939354398
29	20.89585624545641	24.24790338187173	33.77821686207989	21.07802351059197
30	22.123409425588488	26.139618640597263	31.128346864548327	20.608625069265926
31	25.37338640755421	25.047405853415512	29.411342722431876	20.167865016598405
32	26.86246964584989	25.2340919964459	28.69786838881103	19.205569968893172
33	26.524740009184622	25.60667347887509	27.741504368342774	20.127082143597512
34	22.11578381914095	26.39256283076068	29.457491762932886	22.03416158716548
35	22.88760816357886	25.788614800115912	29.832841058072663	21.490935978232564
36	26.348729715180774	26.533099933290067	26.95476772685191	20.163402624677254
37	22.921160831948015	29.061920489213723	28.476160942095664	19.540757736742602
38	22.74029274420722	28.822194016892695	27.152694578645704	21.28481866025438
39	23.384628246037085	28.553829155913153	27.31689930414929	20.744643293900474
40	24.578176870010523	26.0230315909105	27.569730522365337	21.829061016713634
41	20.63771534571393	25.640395605165306	29.06056482584527	24.661324223275496
42	22.419734843542326	26.3851066822342	26.957761483457237	24.23739699076624
43	22.45678964227998	25.925536800329425	28.130579755088114	23.487093802302482
44	21.781782256738918	27.66942826592015	28.61025864362491	21.938530833716026
45	20.576541036212593	29.898647217416208	26.914719171508928	22.61009257486227
46	21.80386827244993	29.196526564506136	27.466417676494657	21.533187486549277
47	22.257563613091417	27.510024848179825	28.429221097963058	21.8031904407657
48	22.43984385017434	25.73269368616732	30.099285395946907	21.72817706771143
49	22.025462747217926	26.01478463875243	29.771497290650274	22.188255323379373
50	20.892184657166858	29.06417992816114	28.426340313305104	21.617295101366903
51	20.54643401223825	29.45076993206432	27.570238896128508	22.432557159568923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1116.0
1	2050.5
2	2985.0
3	15025.0
4	27065.0
5	19686.0
6	12307.0
7	12319.5
8	12332.0
9	12911.0
10	13490.0
11	13474.0
12	13458.0
13	13202.5
14	12947.0
15	12625.5
16	12304.0
17	11665.5
18	11027.0
19	10592.0
20	10157.0
21	10123.0
22	10089.0
23	10129.5
24	10170.0
25	11624.5
26	14739.5
27	16400.0
28	18835.5
29	21271.0
30	24131.0
31	26991.0
32	31649.0
33	36307.0
34	43215.0
35	50123.0
36	53425.5
37	56728.0
38	61319.5
39	65911.0
40	78295.0
41	90679.0
42	102575.5
43	114472.0
44	118428.5
45	122385.0
46	123549.0
47	124713.0
48	121984.0
49	119255.0
50	115546.5
51	111838.0
52	107681.5
53	103525.0
54	98265.5
55	93006.0
56	90139.5
57	87273.0
58	84828.0
59	82383.0
60	78532.0
61	74681.0
62	67910.0
63	61139.0
64	54823.0
65	48507.0
66	41236.0
67	33965.0
68	28861.0
69	23757.0
70	20130.5
71	16504.0
72	13629.0
73	10754.0
74	9026.0
75	5635.0
76	3972.0
77	3065.0
78	2158.0
79	1557.5
80	957.0
81	696.5
82	436.0
83	355.5
84	275.0
85	178.5
86	82.0
87	66.5
88	51.0
89	36.0
90	21.0
91	12.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1770351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.888332963257977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5301363322385	17.287589091325433
2	5.790362672962885	2.6506349767358994
3	2.6126587087959448	1.7939820733883176
4	1.6875095945607144	1.544971259159924
5	1.1353970513281955	1.299367287815052
6	0.9067005182775547	1.245171801617715
7	0.7793876251330328	1.2487218430041422
8	0.648321333821538	1.187119564455271
9	0.6202119161808303	1.2776055160794382
>10	9.00161402768366	48.70626337369115
>50	1.2113692924025248	18.099167067373923
>100	0.07483424201361187	2.470987059647137
>500	7.483423005286125E-4	0.13788287734716426
>1k	4.988948670190751E-4	0.18350127030798588
>5k	0.0	0.0
>10k+	2.4944743350953754E-4	0.8670349380513609
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15186	0.8577959963871571	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2034	0.11489247047619372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02112575415835617	0.0
2	0.0	0.0	0.0	0.06552372947511538	0.0
3	0.0	0.0	0.0	0.10625011650232073	0.0
4	0.0	0.0	0.0	0.15957287566138015	0.0
5	0.0	0.0	0.0	0.2903379047431837	0.0
6	0.0	0.0	0.0	0.5228906584061579	0.0
7	0.0	0.0	0.0	0.652582453987938	0.0
8	0.0	0.0	0.0	0.8910662348878838	0.0
9	0.0	0.0	0.0	1.0418272986543347	0.0
10	0.0	0.0	0.0	1.2378901133165119	0.0
11	0.0	0.0	0.0	1.3821552901091365	0.0
12	0.0	0.0	0.0	1.4970477605853303	0.0
13	0.0	0.0	0.0	1.5579396402182393	0.0
14	0.0	0.0	0.0	1.5877642343241538	0.0
15	0.0	0.0	0.0	1.6233503977459838	0.0
16	0.0	0.0	0.0	1.68452470724732	0.0
17	0.0	0.0	0.0	1.7479019697223883	0.0
18	0.0	0.0	0.0	1.8317271546715876	0.0
19	0.0	0.0	0.0	1.8802486060673844	0.0
20	0.0	0.0	0.0	1.9319897579632515	0.0
21	0.0	0.0	0.0	1.9996034684647281	0.0
22	0.0	0.0	0.0	2.0752946732032234	0.0
23	5.6485973685444296E-5	0.0	0.0	2.1579336527050286	0.0
24	5.6485973685444296E-5	0.0	0.0	2.2259427650223036	0.0
25	5.6485973685444296E-5	0.0	0.0	2.2861003269973015	0.0
26	5.6485973685444296E-5	0.0	0.0	2.347444094419694	0.0
27	5.6485973685444296E-5	0.0	0.0	2.411499188578988	0.0
28	1.1297194737088859E-4	0.0	0.0	2.4779266936330706	0.0
29	1.1297194737088859E-4	0.0	0.0	2.5529400666873405	0.0
30	1.1297194737088859E-4	0.0	0.0	2.6474975866367743	0.0
31	1.1297194737088859E-4	5.6485973685444296E-5	0.0	2.7261825479805983	0.0
32	1.1297194737088859E-4	5.6485973685444296E-5	0.0	2.8123236578509005	0.0
33	1.1297194737088859E-4	5.6485973685444296E-5	0.0	2.893832917878997	0.0
34	1.1297194737088859E-4	5.6485973685444296E-5	0.0	2.9844364196704496	0.0
35	1.1297194737088859E-4	5.6485973685444296E-5	0.0	3.0834563315410333	0.0
36	1.1297194737088859E-4	5.6485973685444296E-5	0.0	3.1695409554376504	0.0
37	1.1297194737088859E-4	5.6485973685444296E-5	0.0	3.2643809052555115	0.0
38	1.1297194737088859E-4	5.6485973685444296E-5	0.0	3.3711958814946867	0.0
39	1.1297194737088859E-4	5.6485973685444296E-5	0.0	3.5077224798924056	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	20	7.034321E-4	45.0	18
CGTTTTT	11240	0.0	41.817173	1
ATAACGG	135	0.0	38.333332	2
TATACGC	30	1.1401711E-4	37.499996	44
ATAGGGC	905	0.0	37.292816	4
CGTTCGG	400	0.0	37.125	45
TATGACG	110	0.0	36.81818	1
AGGGCGA	2155	0.0	36.542923	6
GGGCGAT	3985	0.0	36.53074	7
ACGGGAT	425	0.0	36.52941	5
CGCGACC	345	0.0	36.52174	10
CGACCCG	310	0.0	36.29032	32
CGTACAC	25	0.0021074156	36.0	37
GTTTTTT	14195	0.0	35.648117	2
TACGGGT	165	0.0	35.454544	4
ATGACGG	275	0.0	35.18182	2
TACGGGA	355	0.0	34.859158	4
CTAAGCG	110	0.0	34.772728	1
GGCGATA	880	0.0	34.772728	8
AATACGA	65	3.6743586E-10	34.615383	27
>>END_MODULE