FastQCFastQC Report
Thu 26 May 2016
SRR1545417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545417_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences194059
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.34216398105730733No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5960.30712309143095656No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4210.21694433136314212No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2090.10769920488098982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG302.1561518E-645.0000041
TTACGGG302.1561518E-645.0000043
ATAGTTA551.8189894E-1245.00000437
TGGTATG302.1561518E-645.0000041
CTAGCGG302.1561518E-645.0000042
AAGTACA253.8787897E-545.044
ACTGCGG253.8787897E-545.02
ATGTGCG207.018785E-445.01
CGTTCGG207.018785E-445.045
TCTAGCG253.8787897E-545.01
AAAGTAG207.018785E-445.01
TGGCACA207.018785E-445.022
GTTAACA207.018785E-445.09
CTACGAA502.1827873E-1145.011
CTTGCGG207.018785E-445.02
ATGACGG207.018785E-445.02
GCGATAT253.8787897E-545.09
TAAGGTT207.018785E-445.01
TACTAAG207.018785E-445.01
TGGGCAC253.8787897E-545.06