##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545417_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	194059
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298038225488124	31.0	31.0	33.0	30.0	34.0
2	31.6416193013465	31.0	31.0	34.0	30.0	34.0
3	31.73876501476355	31.0	31.0	34.0	30.0	34.0
4	35.44328271298935	37.0	35.0	37.0	33.0	37.0
5	35.2962707217908	37.0	35.0	37.0	33.0	37.0
6	35.37191266573568	37.0	35.0	37.0	33.0	37.0
7	35.80517780674949	37.0	35.0	37.0	35.0	37.0
8	35.84862850988617	37.0	35.0	37.0	35.0	37.0
9	37.49788466394241	39.0	37.0	39.0	35.0	39.0
10	36.89051783220567	39.0	37.0	39.0	32.0	39.0
11	36.49524629107643	39.0	35.0	39.0	32.0	39.0
12	35.55496008945733	37.0	35.0	39.0	31.0	39.0
13	35.11912356551358	37.0	35.0	39.0	30.0	39.0
14	36.07107116907745	38.0	35.0	40.0	30.0	41.0
15	36.353742933850015	38.0	35.0	40.0	31.0	41.0
16	36.569579354732326	38.0	35.0	40.0	32.0	41.0
17	36.5218103772564	38.0	35.0	40.0	31.0	41.0
18	36.481734936282265	38.0	35.0	40.0	31.0	41.0
19	36.41929516281131	37.0	35.0	40.0	31.0	41.0
20	36.25542747308808	37.0	35.0	40.0	31.0	41.0
21	36.08400022673517	37.0	34.0	40.0	31.0	41.0
22	35.978315873007695	37.0	34.0	40.0	30.0	41.0
23	35.925383517383885	36.0	34.0	40.0	30.0	41.0
24	35.856667302212216	36.0	34.0	40.0	30.0	41.0
25	35.744510690047875	36.0	34.0	40.0	30.0	41.0
26	35.62740712875981	36.0	34.0	40.0	30.0	41.0
27	35.56399342468012	36.0	34.0	40.0	30.0	41.0
28	35.603677232181965	36.0	34.0	40.0	30.0	41.0
29	35.64857079547973	36.0	34.0	40.0	30.0	41.0
30	35.55974729334893	36.0	34.0	40.0	30.0	41.0
31	35.37914757882912	36.0	34.0	40.0	29.0	41.0
32	35.21051845057431	36.0	34.0	40.0	29.0	41.0
33	35.029351898134074	36.0	34.0	40.0	27.0	41.0
34	34.885843995898156	36.0	34.0	40.0	27.0	41.0
35	34.717163336923306	35.0	34.0	40.0	27.0	41.0
36	34.527159265996424	35.0	33.0	40.0	25.0	41.0
37	34.44629210703961	35.0	33.0	40.0	25.0	41.0
38	34.447296956080365	35.0	33.0	40.0	25.0	41.0
39	34.416296074905055	35.0	33.0	40.0	25.0	41.0
40	34.225900370505876	35.0	33.0	40.0	24.0	41.0
41	34.203479354217016	35.0	33.0	40.0	24.0	41.0
42	34.12482801622187	35.0	33.0	40.0	24.0	41.0
43	34.03145950458366	35.0	33.0	40.0	23.0	41.0
44	33.97753260606311	35.0	33.0	40.0	23.0	41.0
45	33.943465647045485	35.0	33.0	40.0	23.0	41.0
46	33.92572362013615	35.0	33.0	39.0	23.0	41.0
47	33.85950149181434	35.0	33.0	39.0	23.0	41.0
48	33.85125142353614	35.0	33.0	39.0	23.0	41.0
49	33.80804291478365	35.0	33.0	39.0	24.0	41.0
50	33.680215810655525	35.0	33.0	39.0	24.0	41.0
51	33.53316259488094	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	6.0
16	23.0
17	47.0
18	124.0
19	219.0
20	412.0
21	618.0
22	859.0
23	1285.0
24	1750.0
25	2535.0
26	3327.0
27	3818.0
28	4357.0
29	4978.0
30	5874.0
31	7322.0
32	8806.0
33	11283.0
34	19732.0
35	30367.0
36	13249.0
37	15879.0
38	21693.0
39	35460.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.859382971158258	24.425561298368024	29.50236783658578	14.21268789388794
2	29.75486836477566	26.82328570177111	30.38715029965114	13.034695633802091
3	28.644381347940573	25.38506330548957	31.76559706068773	14.20495828588213
4	25.809676438608875	28.24811011084258	30.70715607109178	15.235057379456762
5	23.22025775666163	32.41694536197755	29.48330147017144	14.879495411189382
6	21.93095914129208	40.86849875553311	26.778453975337396	10.42208812783741
7	86.02847587589342	5.13039848705806	6.876774589171335	1.9643510478771922
8	87.84544906445979	4.052375823847386	6.139885292617193	1.9622898190756421
9	82.52077976285562	5.918303196450564	8.443308478349367	3.117608562344442
10	53.420866849772494	21.54138689779912	13.897319887250784	11.140426365177602
11	47.114537331430135	19.788827109281197	19.37400481296925	13.72263074631942
12	43.025574696355235	19.924352902983113	23.785034448286346	13.265037952375309
13	21.57436655862392	40.98083572521759	22.609103417002046	14.835694299156444
14	15.704502239009784	42.28095579179528	26.01786054756543	15.996681421629505
15	14.618749967793299	23.08627788456088	47.386619533234736	14.90835261441108
16	15.936905786384553	17.835297512612144	46.175132305123704	20.052664395879603
17	15.588558118922597	19.422958997006067	28.708279440788626	36.28020344328271
18	21.623836049861126	22.64775145703111	34.67656743567678	21.051845057430988
19	29.810006235217124	23.84841723393401	25.129470934097363	21.212105596751503
20	32.27729711067253	21.350207926455354	25.762783483373614	20.6097114794985
21	21.6042543762464	28.834014397683177	27.165964990028808	22.395766236041617
22	22.71525670028187	25.186670033340373	24.11173921333203	27.986334053045724
23	20.047511323875728	30.629859991033655	23.92674392839291	25.395884756697708
24	20.271154648843908	22.162332074266075	40.207359617435934	17.359153659454083
25	17.342663829041683	25.227379302170988	35.805605511725815	21.62435135706151
26	17.5482714019963	33.92834138071411	27.22316408927182	21.300223128017766
27	17.87909862464508	34.71366955410468	28.267176477256918	19.140055343993325
28	15.677706264589636	28.30221736688327	37.96062022374639	18.05945614478071
29	15.776645247064035	23.52377369768988	36.96556201979811	23.734019035447982
30	18.489737657104282	31.91039838399662	29.73219484795861	19.867669110940486
31	28.880907352918445	27.54420047511324	24.567786085674975	19.007106086293344
32	30.84216655759331	25.23150175977409	26.26881515415415	17.657516528478453
33	28.524314770250285	27.743109054462817	24.535321732050562	19.197254443236336
34	20.274761799246622	26.26623861815221	28.40579411416116	25.05320546844001
35	20.74884442360313	25.25623650539269	31.14207534821884	22.852843722785337
36	30.238741825939535	25.88542659706584	24.711041487382705	19.164790089611923
37	20.061424618286193	33.423855631534735	28.78042244884288	17.73429730133619
38	20.16757790156602	32.91936988235536	25.31395091183609	21.599101304242524
39	21.169850406319725	33.01109456402435	26.206978290107646	19.612076739548282
40	25.639625062481	26.281697834163836	25.09443004447101	22.98424705888415
41	16.90671393751385	24.945506263559018	27.51946572949464	30.628314069432495
42	21.50016232176812	25.083608593262873	24.414739847159883	29.001489237809118
43	21.910862160476967	26.235320186128963	25.85708470104453	25.996732952349543
44	19.534780659490156	29.379724722893553	28.819585796072328	22.265908821543963
45	16.9293874543309	37.91630380451306	22.67815458185397	22.476154159302066
46	20.96218160456356	34.25092368815669	25.54790038081202	19.238994326467722
47	21.478004112151456	27.522042265496577	27.018587130717975	23.981366491633988
48	22.934262260446562	23.435656166423612	32.0763273025214	21.553754270608426
49	20.805528215645758	24.118953514137452	32.63904276534456	22.43647550487223
50	19.095738924759996	33.58102432765293	27.386516471794657	19.936720275792414
51	18.93341715663793	34.09684683524083	23.849447848334783	23.120288159786455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	193.0
2	299.0
3	762.0
4	1225.0
5	925.0
6	625.0
7	652.5
8	680.0
9	721.0
10	762.0
11	779.0
12	796.0
13	877.5
14	959.0
15	912.5
16	866.0
17	797.5
18	729.0
19	737.0
20	745.0
21	734.0
22	723.0
23	751.5
24	780.0
25	863.0
26	1218.5
27	1491.0
28	1922.0
29	2353.0
30	2719.0
31	3085.0
32	3545.0
33	4005.0
34	4728.0
35	5451.0
36	5758.5
37	6066.0
38	7180.5
39	8295.0
40	11072.5
41	13850.0
42	16681.0
43	19512.0
44	20090.0
45	20668.0
46	19114.0
47	17560.0
48	16155.0
49	14750.0
50	13692.5
51	12635.0
52	11642.0
53	10649.0
54	9832.0
55	9015.0
56	8173.5
57	7332.0
58	6704.5
59	6077.0
60	5666.0
61	5255.0
62	4903.0
63	4551.0
64	4060.5
65	3570.0
66	3039.0
67	2508.0
68	2198.0
69	1888.0
70	1532.0
71	1176.0
72	1002.5
73	829.0
74	710.5
75	457.0
76	322.0
77	237.0
78	152.0
79	143.5
80	135.0
81	91.0
82	47.0
83	29.0
84	11.0
85	8.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	194059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.27017556516317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.28746042014232	20.36803240251676
2	6.833270913486092	4.1368862047109385
3	3.8643559974124138	3.50924203463895
4	3.0301998570018043	3.668987266759079
5	2.5007660617616017	3.7849313868462686
6	2.058152599502911	3.738038431611005
7	1.8130128357904056	3.8416151788888944
8	1.598515542541963	3.8709876893109825
9	1.344863981478329	3.6638341947552036
>10	9.633652242007422	47.80607959435017
>50	0.02553539205338599	0.49160306916968555
>100	0.006809437880902931	0.4704754739537975
>500	0.0034047189404514656	0.6492870724882639
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	664	0.34216398105730733	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	596	0.30712309143095656	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	421	0.21694433136314212	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	209	0.10769920488098982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022673516817050484	0.0
2	0.0	0.0	0.0	0.09223998886936448	0.0
3	0.0	0.0	0.0	0.1303727216980403	0.0
4	0.0	0.0	0.0	0.24425561298368023	0.0
5	0.0	0.0	0.0	0.4199753683158215	0.0
6	0.0	0.0	0.0	0.6487717652878764	0.0
7	0.0	0.0	0.0	0.7791444869859167	0.0
8	0.0	0.0	0.0	1.1285227688486492	0.0
9	0.0	0.0	0.0	1.2764159353598648	0.0
10	0.0	0.0	0.0	1.543860372360983	0.0
11	0.0	0.0	0.0	1.7494679453156	0.0
12	0.0	0.0	0.0	1.9303407726516162	0.0
13	0.0	0.0	0.0	2.0045450095074178	0.0
14	0.0	0.0	0.0	2.030825676727181	0.0
15	0.0	0.0	0.0	2.065351259153144	0.0
16	0.0	0.0	0.0	2.149861640016696	0.0
17	0.0	0.0	0.0	2.2338567136798604	0.0
18	0.0	0.0	0.0	2.3601069777748003	5.153072003875111E-4
19	0.0	0.0	0.0	2.4106070834127764	5.153072003875111E-4
20	0.0	0.0	0.0	2.477597019463153	5.153072003875111E-4
21	0.0	0.0	0.0	2.564168629128255	5.153072003875111E-4
22	0.0	0.0	0.0	2.6548626963964566	5.153072003875111E-4
23	0.0	0.0	0.0	2.762046594077059	5.153072003875111E-4
24	0.0	0.0	0.0	2.846041667740223	5.153072003875111E-4
25	0.0	0.0	0.0	2.912516296590212	5.153072003875111E-4
26	0.0	0.0	0.0	2.971776624634776	5.153072003875111E-4
27	0.0	0.0	0.0	3.0346441030820523	5.153072003875111E-4
28	0.0	0.0	0.0	3.1057564967355287	5.153072003875111E-4
29	0.0	0.0	0.0	3.181506655192493	5.153072003875111E-4
30	0.0	0.0	0.0	3.2768384872641825	5.153072003875111E-4
31	0.0	0.0	0.0	3.3732009337366473	5.153072003875111E-4
32	0.0	0.0	0.0	3.4654409226060117	5.153072003875111E-4
33	0.0	0.0	0.0	3.542737002664138	5.153072003875111E-4
34	0.0	0.0	0.0	3.636522913134665	5.153072003875111E-4
35	0.0	0.0	0.0	3.7679262492334806	5.153072003875111E-4
36	0.0	0.0	0.0	3.8658346173071076	5.153072003875111E-4
37	0.0	0.0	0.0	3.9786868941919726	5.153072003875111E-4
38	0.0	0.0	0.0	4.083809563071025	5.153072003875111E-4
39	0.0	0.0	0.0	4.1755342447400015	5.153072003875111E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	30	2.1561518E-6	45.000004	1
TTACGGG	30	2.1561518E-6	45.000004	3
ATAGTTA	55	1.8189894E-12	45.000004	37
TGGTATG	30	2.1561518E-6	45.000004	1
CTAGCGG	30	2.1561518E-6	45.000004	2
AAGTACA	25	3.8787897E-5	45.0	44
ACTGCGG	25	3.8787897E-5	45.0	2
ATGTGCG	20	7.018785E-4	45.0	1
CGTTCGG	20	7.018785E-4	45.0	45
TCTAGCG	25	3.8787897E-5	45.0	1
AAAGTAG	20	7.018785E-4	45.0	1
TGGCACA	20	7.018785E-4	45.0	22
GTTAACA	20	7.018785E-4	45.0	9
CTACGAA	50	2.1827873E-11	45.0	11
CTTGCGG	20	7.018785E-4	45.0	2
ATGACGG	20	7.018785E-4	45.0	2
GCGATAT	25	3.8787897E-5	45.0	9
TAAGGTT	20	7.018785E-4	45.0	1
TACTAAG	20	7.018785E-4	45.0	1
TGGGCAC	25	3.8787897E-5	45.0	6
>>END_MODULE