FastQCFastQC Report
Thu 26 May 2016
SRR1545416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545416_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6158110
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220320.35777210865021897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC219230.35600208505531733No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC253.8927938E-545.039
CGCGATA253.8927938E-545.011
AATGCGT351.213084E-745.015
TTCGGAC351.213084E-745.04
TCCGGTA253.8927938E-545.018
CTATGCG2150.041.8604661
TACGATG1950.041.538461
TCGCTAG2800.040.982141
GTCGACG556.184564E-1140.9090921
CGTTTTT211150.040.833531
TTATACG3550.040.5633771
ACGCGTA3850.040.32467723
ACCGCGA451.9306754E-840.00000413
CGTTAGG5650.039.8230062
TAACGCG1250.039.61
CGCTAGG6100.039.4672132
CGACGGT4000.039.37528
CGGTCGA403.4615005E-739.3756
GTACGTC403.4615005E-739.37542
AGGGCGA64150.039.353086
Produced by FastQC (version 0.11.5)