##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545416_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	6158110
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274420723241384	31.0	31.0	33.0	30.0	34.0
2	31.61827947211076	31.0	31.0	34.0	30.0	34.0
3	31.70570028791301	31.0	31.0	34.0	30.0	34.0
4	35.413181804157446	37.0	35.0	37.0	33.0	37.0
5	35.26521871158521	37.0	35.0	37.0	33.0	37.0
6	35.331879748819034	37.0	35.0	37.0	32.0	37.0
7	35.849508047111854	37.0	35.0	37.0	35.0	37.0
8	35.890446257049646	37.0	35.0	37.0	35.0	37.0
9	37.552804513073006	39.0	37.0	39.0	35.0	39.0
10	36.81135153480532	39.0	37.0	39.0	32.0	39.0
11	36.470698964455	39.0	35.0	39.0	32.0	39.0
12	35.647692067858486	37.0	35.0	39.0	31.0	39.0
13	35.279396762967856	37.0	35.0	39.0	30.0	39.0
14	36.23337452562556	38.0	35.0	40.0	30.0	41.0
15	36.49639516020338	38.0	35.0	40.0	31.0	41.0
16	36.68534647805902	38.0	35.0	40.0	32.0	41.0
17	36.642969190222324	38.0	35.0	40.0	32.0	41.0
18	36.58413279399036	38.0	35.0	40.0	31.0	41.0
19	36.52080134976478	37.0	35.0	40.0	31.0	41.0
20	36.3858316918665	37.0	35.0	40.0	31.0	41.0
21	36.19525893496544	37.0	35.0	40.0	31.0	41.0
22	36.11515952134665	37.0	35.0	40.0	30.0	41.0
23	36.06007313932359	37.0	34.0	40.0	30.0	41.0
24	36.006108042889785	37.0	34.0	40.0	30.0	41.0
25	35.904727424485756	37.0	34.0	40.0	30.0	41.0
26	35.798932951831	37.0	34.0	40.0	30.0	41.0
27	35.76341556094321	37.0	34.0	40.0	30.0	41.0
28	35.76517129443936	37.0	34.0	40.0	30.0	41.0
29	35.800741948422484	37.0	34.0	40.0	30.0	41.0
30	35.71864922841586	36.0	34.0	40.0	30.0	41.0
31	35.56419193551268	36.0	34.0	40.0	30.0	41.0
32	35.37546438761243	36.0	34.0	40.0	29.0	41.0
33	35.19782189665336	36.0	34.0	40.0	28.0	41.0
34	35.04113242537077	36.0	34.0	40.0	27.0	41.0
35	34.89827170998894	36.0	34.0	40.0	27.0	41.0
36	34.71500638994757	36.0	34.0	40.0	25.0	41.0
37	34.65358803269185	36.0	33.0	40.0	25.0	41.0
38	34.66090829816291	36.0	33.0	40.0	26.0	41.0
39	34.625467878943375	36.0	33.0	40.0	25.0	41.0
40	34.45413657761878	36.0	33.0	40.0	24.0	41.0
41	34.446079722512266	36.0	33.0	40.0	24.0	41.0
42	34.39083744850287	36.0	33.0	40.0	24.0	41.0
43	34.30715268158575	35.0	33.0	40.0	24.0	41.0
44	34.26594572035901	35.0	33.0	40.0	24.0	41.0
45	34.23332775802965	35.0	33.0	40.0	24.0	41.0
46	34.21690340055634	35.0	33.0	40.0	24.0	41.0
47	34.171129778454755	35.0	33.0	40.0	24.0	41.0
48	34.156573851392714	35.0	33.0	40.0	24.0	41.0
49	34.112949914827766	36.0	33.0	40.0	24.0	41.0
50	33.985834127678785	35.0	33.0	40.0	24.0	41.0
51	33.805442903748066	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	8.0
13	16.0
14	61.0
15	186.0
16	586.0
17	1564.0
18	3575.0
19	6798.0
20	11573.0
21	18235.0
22	27891.0
23	39961.0
24	56645.0
25	79233.0
26	103542.0
27	119750.0
28	132051.0
29	150260.0
30	179953.0
31	219636.0
32	272237.0
33	349459.0
34	583232.0
35	907782.0
36	425856.0
37	505693.0
38	720917.0
39	1240792.0
40	614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.96305359923743	24.135538338873452	29.817314078507856	14.084093983381266
2	30.473229611033254	25.46221162012371	30.648461946928524	13.416096821914516
3	27.32552682560071	25.187646859182443	32.61245739358342	14.874368921633424
4	24.85066034871089	28.210993308011712	31.904935118080058	15.03341122519734
5	22.677282477903123	32.22883319719849	30.639238337736742	14.454645987161646
6	20.85219328657656	41.56182659939495	27.55981949007082	10.026160623957676
7	86.31787999889576	4.799134799475813	7.040244490598576	1.842740711029845
8	87.7357500921549	3.5960059173999817	6.647948802473485	2.020295187971634
9	82.83289840551727	5.655663831922457	8.511264007950492	3.000173754609775
10	49.23585320820836	21.712554663687396	17.546146463768917	11.505445664335323
11	44.726271534610454	22.922763640142836	19.220328964568676	13.130635860678034
12	38.864375595759086	22.473486183260775	24.77464676662158	13.88749145435856
13	21.06906177382346	41.296047001433884	23.99348176632116	13.641409458421498
14	16.44101842935576	43.255414404744315	26.43202216264406	13.87154500325587
15	14.604724501511015	25.121928643691007	47.06223175617194	13.211115098626038
16	15.719741933807613	20.274386134706916	45.70554926755124	18.300322663934228
17	16.24561756772776	20.67556441830367	29.19012489221531	33.88869312175326
18	20.632872748294524	24.485304744475172	35.39590880968349	19.48591369754681
19	27.595950705654822	26.106142956199225	26.335775099827707	19.96213123831825
20	30.19351716679306	23.677410764016884	26.609365535854344	19.519706533335714
21	21.5788610466523	29.62350461424041	28.22377320314187	20.57386113596542
22	22.419167569270442	26.5127612205693	25.841435115644245	25.226636094516014
23	18.971551336367813	31.20842271411196	25.41328427066097	24.406741678859262
24	19.048165752154475	24.677961257593644	39.14608540607426	17.12778758417761
25	16.60483492500134	26.708227037191605	36.112362396904246	20.574575640902808
26	16.78984298754001	34.79179488511897	28.616020175021234	19.802341952319786
27	17.36752022942104	35.87358783782687	28.675730053539155	18.08316187921294
28	14.883592530825204	30.78537083618188	37.14298705284576	17.188049580147155
29	15.679128823616336	25.920680208700396	36.38580668419369	22.014384283489576
30	17.223287664559418	33.32217190014469	31.079649437895718	18.374890997400176
31	26.191932264931932	28.75188978436566	25.87587750137623	19.180300449326175
32	27.023031417106875	28.041655637849928	27.474842118766958	17.460470826276246
33	26.655759640539063	29.682808524043903	24.550227261286338	19.1112045741307
34	18.978663908244574	29.62100384695954	27.71853701866319	23.681795226132692
35	19.218104256013614	28.16968193163162	30.153163876579015	22.45904993577575
36	27.524906180630094	27.71433118278173	26.072918476610518	18.687844159977658
37	19.189637729757994	34.130861579283255	29.066466821800844	17.613033869157906
38	19.33174301855602	33.299421413388195	25.590546450128365	21.778289117927415
39	19.724883121607117	33.64832716531534	27.398406329214648	19.22838338386291
40	23.37358377813972	27.452887330690746	26.43806297711473	22.7354659140548
41	17.17211936779304	26.313966460488693	28.162715508492052	28.351198663226217
42	20.641316897554606	26.5539914032065	26.19128271498885	26.613408984250036
43	20.802973639639436	26.992535047279116	27.342009155406448	24.862482157675
44	19.235625865728284	30.697080760168298	28.670647325234526	21.396646048868888
45	16.376583075001907	38.641904740253096	23.501220341955566	21.480291842789427
46	20.587095716055735	34.904199502769515	25.5732359441452	18.935468837029543
47	20.511163327709315	29.460045371063526	26.71443023914805	23.31436106207911
48	21.531622527041577	26.397173808197643	30.145353038513438	21.92585062624734
49	20.509815511577415	25.947019458892417	32.13901667881866	21.404148350711502
50	19.115637752492244	34.22459163607016	26.37523525887001	20.28453535256759
51	18.020626458442607	34.168779057210735	24.50188450677237	23.30870997757429
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3416.0
1	5412.0
2	7408.0
3	22405.5
4	37403.0
5	28509.5
6	19616.0
7	21455.0
8	23294.0
9	25852.0
10	28410.0
11	29668.0
12	30926.0
13	30782.5
14	30639.0
15	29459.0
16	28279.0
17	27399.5
18	26520.0
19	26501.5
20	26483.0
21	27110.0
22	27737.0
23	28009.5
24	28282.0
25	33092.0
26	44798.0
27	51694.0
28	63562.0
29	75430.0
30	95972.5
31	116515.0
32	133233.0
33	149951.0
34	175229.0
35	200507.0
36	214643.0
37	228779.0
38	272428.0
39	316077.0
40	403816.0
41	491555.0
42	574936.5
43	658318.0
44	672615.5
45	686913.0
46	655535.0
47	624157.0
48	563929.5
49	503702.0
50	469987.0
51	436272.0
52	385821.0
53	335370.0
54	298547.5
55	261725.0
56	229570.5
57	197416.0
58	170903.5
59	144391.0
60	129152.0
61	113913.0
62	94770.5
63	75628.0
64	61285.5
65	46943.0
66	39235.5
67	31528.0
68	26040.0
69	20552.0
70	16877.5
71	13203.0
72	11453.5
73	9704.0
74	7366.5
75	4002.0
76	2975.0
77	2616.0
78	2257.0
79	1639.0
80	1021.0
81	596.5
82	172.0
83	103.5
84	35.0
85	31.0
86	27.0
87	27.5
88	28.0
89	15.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6158110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.585796086648209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41703372335805	11.437748636087166
2	9.144723794059685	2.6676615305774907
3	3.2830193490218886	1.4365635232006149
4	1.7197932976791848	1.003382174045315
5	1.038430569813823	0.7573168270723164
6	0.7558677608693063	0.6614959817106644
7	0.543168218386826	0.5545778611896774
8	0.4355178922081761	0.5081900134268234
9	0.31517412713192827	0.41373589951202794
>10	2.312099863987046	7.051821768336946
>50	0.5441555683721575	5.7442227396143455
>100	1.255557376218567	43.93927921941305
>500	0.22361787547198259	20.771561611047403
>1k	0.011502281037893173	2.2436760364276087
>5k	1.1276746115581542E-4	0.08579293457962325
>10k+	2.2553492231163085E-4	0.722973243759076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22032	0.35777210865021897	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	21923	0.35600208505531733	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044737752329854455	0.0
2	0.0	0.0	0.0	0.1538134265220985	0.0
3	0.0	0.0	0.0	0.2215777243342519	0.0
4	0.0	0.0	0.0	0.36149078207437024	0.0
5	0.0	0.0	0.0	0.6146203948938879	0.0
6	0.0	0.0	0.0	0.911464718882904	0.0
7	0.0	0.0	0.0	1.0992983236739844	0.0
8	0.0	0.0	0.0	1.5683545763229303	0.0
9	0.0	0.0	0.0	1.7506670065978036	0.0
10	0.0	0.0	0.0	2.059771585762515	0.0
11	1.6238748577079656E-5	0.0	0.0	2.3425206759866257	0.0
12	1.6238748577079656E-5	0.0	0.0	2.5917529891476443	0.0
13	3.247749715415931E-5	0.0	0.0	2.6886496019070787	0.0
14	3.247749715415931E-5	0.0	0.0	2.725576516171358	0.0
15	3.247749715415931E-5	0.0	0.0	2.78255828492833	0.0
16	4.8716245731238965E-5	0.0	0.0	2.9150827120658773	0.0
17	4.8716245731238965E-5	0.0	0.0	3.0692371523080944	0.0
18	4.8716245731238965E-5	0.0	0.0	3.289467060510449	0.0
19	4.8716245731238965E-5	0.0	0.0	3.388767008059291	0.0
20	6.495499430831862E-5	0.0	0.0	3.4980050697373057	0.0
21	6.495499430831862E-5	0.0	0.0	3.644023896942406	0.0
22	6.495499430831862E-5	0.0	0.0	3.7984543959104338	0.0
23	6.495499430831862E-5	0.0	0.0	3.988350321770803	0.0
24	6.495499430831862E-5	0.0	0.0	4.126866847133292	0.0
25	8.119374288539828E-5	0.0	0.0	4.243850791882575	0.0
26	9.743249146247793E-5	0.0	0.0	4.366891789851107	0.0
27	9.743249146247793E-5	0.0	0.0	4.482300575988412	0.0
28	9.743249146247793E-5	0.0	0.0	4.603539072864889	0.0
29	9.743249146247793E-5	0.0	0.0	4.736842959934136	0.0
30	9.743249146247793E-5	0.0	0.0	4.911799237103592	0.0
31	1.136712400395576E-4	0.0	0.0	5.073520934182728	0.0
32	1.136712400395576E-4	0.0	0.0	5.22092005501688	0.0
33	1.136712400395576E-4	0.0	0.0	5.367068792210597	0.0
34	1.136712400395576E-4	0.0	0.0	5.520865979984118	0.0
35	1.136712400395576E-4	0.0	0.0	5.719417158836071	0.0
36	1.136712400395576E-4	0.0	0.0	5.88251914954426	0.0
37	1.136712400395576E-4	0.0	0.0	6.060918041412057	0.0
38	1.136712400395576E-4	0.0	0.0	6.2315548114600094	0.0
39	1.136712400395576E-4	0.0	0.0	6.40025917042729	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATC	25	3.8927938E-5	45.0	39
CGCGATA	25	3.8927938E-5	45.0	11
AATGCGT	35	1.213084E-7	45.0	15
TTCGGAC	35	1.213084E-7	45.0	4
TCCGGTA	25	3.8927938E-5	45.0	18
CTATGCG	215	0.0	41.860466	1
TACGATG	195	0.0	41.53846	1
TCGCTAG	280	0.0	40.98214	1
GTCGACG	55	6.184564E-11	40.909092	1
CGTTTTT	21115	0.0	40.83353	1
TTATACG	355	0.0	40.563377	1
ACGCGTA	385	0.0	40.324677	23
ACCGCGA	45	1.9306754E-8	40.000004	13
CGTTAGG	565	0.0	39.823006	2
TAACGCG	125	0.0	39.6	1
CGCTAGG	610	0.0	39.467213	2
CGACGGT	400	0.0	39.375	28
CGGTCGA	40	3.4615005E-7	39.375	6
GTACGTC	40	3.4615005E-7	39.375	42
AGGGCGA	6415	0.0	39.35308	6
>>END_MODULE