FastQCFastQC Report
Thu 26 May 2016
SRR1545415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545415_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1620238
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC70060.43240560954625185No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.33982661806475345No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19900.1228214620321212No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18830.11621749397310766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG302.1660544E-645.0000041
CGATATC302.1660544E-645.00000410
TCGTTAG1000.045.01
ACACGAA207.034144E-445.042
CGTATGA453.8562575E-1045.015
GTCGAAA351.2123382E-745.039
CGCATCG207.034144E-445.045
ATTACCG207.034144E-445.01
CACGCTA207.034144E-445.015
CTAAGCG351.2123382E-745.01
CGCGAAC207.034144E-445.028
GCGCATC351.2123382E-745.021
GCGTTAG502.1827873E-1145.01
CGTTAGT406.8175723E-945.021
GTAGCAC207.034144E-445.033
CGCTAAG351.2123382E-745.042
ACGTCTT351.2123382E-745.034
CTATTAG502.1827873E-1145.01
ACCGTAT253.8915154E-545.020
CCGCGAA207.034144E-445.027