FastQCFastQC Report
Thu 26 May 2016
SRR1545414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545414_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6613561
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279980.4233422811099799No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC145250.21962449578978707No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT82740.1251065802523028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA207.035721E-445.00000431
GTCGACG556.184564E-1140.9090921
TACGTAG1700.039.7058831
CGTTTTT254350.039.1173551
TAGGGCG24800.039.102825
TGTTACG1850.038.918921
TAACGGG17300.038.1069373
TTCGCGA659.094947E-1238.0769215
TAGGGTA35100.037.9487155
TATCGTG2700.037.5000041
CTATACG1800.037.51
CCGTAGA900.037.534
AGGGCGA58400.037.217476
TTACGCG1950.036.923081
ATAGGGC32350.036.792894
GGACTAA42450.036.784458
TATACGG7100.036.7605632
CGACCCG9400.036.6223432
GCTACGA11500.036.58695610
CGAATAT10750.036.20930514
Produced by FastQC (version 0.11.5)