FastQCFastQC Report
Thu 26 May 2016
SRR1545413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545413_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences777845
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.40277947405974196No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20720.2663769774183803No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13510.173684988654552No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10230.13151720458446092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG302.1645938E-645.0000041
ATAACGG302.1645938E-645.0000042
CCAACGG600.045.0000042
TACGTAG302.1645938E-645.0000041
TGATTCG502.1827873E-1145.015
TACTCGC406.8102963E-945.045
TCGTAAG207.0318783E-445.01
TATAGCG207.0318783E-445.01
TTACGCA207.0318783E-445.029
AATACGG406.8102963E-945.02
ATCGACT253.889638E-545.017
GCGTAAG650.041.5384641
ACGGGTA1750.041.1428575
TCGAACG556.002665E-1140.9090921
AGGGTAT6200.040.645166
AGGGCGC1000.040.56
CGTTTTT26450.040.4064251
CGTAAGG1400.040.1785742
TATGGGC2250.040.04
GCGATAC451.9268555E-840.09