FastQCFastQC Report
Thu 26 May 2016
SRR1545411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545411_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2676327
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97820.3655009272035891No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79090.29551695289850605No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT38850.14516163383622405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG850.045.0000041
CTCGATT600.045.00000420
TTACACG1000.042.7534
ACTCGAT603.6379788E-1241.25000419
CGTAGTA451.9299478E-840.023
ACTCGAC451.9299478E-840.020
GCTACGA5200.039.8076910
CGTTTTT83500.039.745511
CGTTAGG3850.039.7402572
TACGTTC403.4605182E-739.37527
ACGTTAG1450.038.7931061
CGAGGGT5300.038.6320764
ATTACGC356.2514027E-638.57142644
CATTACG356.2514027E-638.57142643
CTACGTT356.2514027E-638.57142645
GGGCGAT51250.038.1073157
TAGGGTA17850.037.8151255
AGGGCGA30450.037.758626
AAACTCG301.140327E-437.5000041
CGGCTAC11850.037.4050625