##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545411_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2676327
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28779517600054	31.0	31.0	33.0	30.0	34.0
2	31.62918357883771	31.0	31.0	34.0	30.0	34.0
3	31.735792001500563	31.0	31.0	34.0	30.0	34.0
4	35.43784597323123	37.0	35.0	37.0	33.0	37.0
5	35.2945114703846	37.0	35.0	37.0	33.0	37.0
6	35.3711153382976	37.0	35.0	37.0	33.0	37.0
7	35.83334809236689	37.0	35.0	37.0	35.0	37.0
8	35.869391146896476	37.0	35.0	37.0	35.0	37.0
9	37.5216466448233	39.0	37.0	39.0	35.0	39.0
10	36.83937874557182	39.0	37.0	39.0	32.0	39.0
11	36.50080277933152	39.0	35.0	39.0	32.0	39.0
12	35.83841137499267	37.0	35.0	39.0	31.0	39.0
13	35.561506497524405	37.0	35.0	39.0	30.0	39.0
14	36.55666777639653	38.0	35.0	41.0	31.0	41.0
15	36.75321588131794	38.0	35.0	41.0	31.0	41.0
16	36.8876650723174	38.0	35.0	41.0	32.0	41.0
17	36.837581506295756	38.0	35.0	41.0	32.0	41.0
18	36.76092607517691	38.0	35.0	40.0	31.0	41.0
19	36.72430611057617	38.0	35.0	40.0	31.0	41.0
20	36.59130143663312	38.0	35.0	40.0	31.0	41.0
21	36.415194406363646	38.0	35.0	40.0	31.0	41.0
22	36.33536036515717	38.0	35.0	40.0	31.0	41.0
23	36.27919533001759	38.0	35.0	40.0	31.0	41.0
24	36.21081205697211	38.0	35.0	40.0	30.0	41.0
25	36.10668688841087	38.0	35.0	40.0	30.0	41.0
26	36.01465067609451	38.0	35.0	40.0	30.0	41.0
27	35.96960909485276	37.0	34.0	40.0	30.0	41.0
28	35.96469900725883	37.0	35.0	40.0	30.0	41.0
29	35.9808031679238	37.0	35.0	40.0	30.0	41.0
30	35.90770634530085	37.0	35.0	40.0	30.0	41.0
31	35.75595620415592	37.0	34.0	40.0	30.0	41.0
32	35.58860819324395	37.0	34.0	40.0	29.0	41.0
33	35.42860943375006	37.0	34.0	40.0	29.0	41.0
34	35.25857228955953	37.0	34.0	40.0	27.0	41.0
35	35.12809122353136	37.0	34.0	40.0	27.0	41.0
36	34.96058628112335	37.0	34.0	40.0	26.0	41.0
37	34.90141600783462	37.0	34.0	40.0	26.0	41.0
38	34.884746893783905	37.0	34.0	40.0	26.0	41.0
39	34.8092243586079	37.0	34.0	40.0	26.0	41.0
40	34.67559756337697	36.0	33.0	40.0	25.0	41.0
41	34.6464221300312	36.0	34.0	40.0	25.0	41.0
42	34.560293641247874	36.0	33.0	40.0	25.0	41.0
43	34.48711312182704	36.0	33.0	40.0	25.0	41.0
44	34.42209453478592	36.0	33.0	40.0	24.0	41.0
45	34.3665221028671	36.0	33.0	40.0	24.0	41.0
46	34.333396853224585	36.0	33.0	40.0	24.0	41.0
47	34.29008787042839	35.0	33.0	40.0	24.0	41.0
48	34.26625184441214	35.0	33.0	40.0	24.0	41.0
49	34.20408604778115	36.0	33.0	40.0	24.0	41.0
50	34.06825436503088	35.0	33.0	40.0	24.0	41.0
51	33.919010644065544	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	7.0
13	10.0
14	18.0
15	67.0
16	232.0
17	604.0
18	1424.0
19	2690.0
20	4652.0
21	7352.0
22	11125.0
23	16275.0
24	22933.0
25	32114.0
26	41893.0
27	49341.0
28	55724.0
29	63925.0
30	75775.0
31	92291.0
32	115229.0
33	147986.0
34	241325.0
35	359170.0
36	197531.0
37	241374.0
38	340022.0
39	554993.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.4889570669055	24.057598342803402	28.53870248291782	13.914742107373277
2	30.167389859310912	26.00459510366259	30.550825814633264	13.277189222393226
3	28.906445288636256	25.376308649877238	31.51150812288633	14.20573793860018
4	26.371179605481693	27.71690454865941	31.185277434334445	14.726638411524451
5	22.68269161429078	32.811648202928865	30.082310569672536	14.423349613107817
6	21.62209625355945	41.00291182654436	27.273087332003897	10.101904587892287
7	85.7979237963074	5.255374249858108	7.050820023113767	1.8958819307207226
8	87.04818207939464	4.337661279806242	6.5427729870079405	2.0713836537911847
9	81.89668153405768	6.295120140401378	8.331978865063947	3.47621946047699
10	47.82677901467197	22.406492181261857	16.895954791772454	12.870774012293715
11	41.08167649169926	23.70102756501728	20.166892909573455	15.050403033710005
12	35.93406186912137	22.291334354882643	26.726778902578047	15.047824873417934
13	22.046297033210067	37.03149876677999	25.264775193763693	15.657429006246248
14	17.924603383667243	38.143358416217445	28.106655128465242	15.825383071650064
15	17.567546865536237	24.02804291104936	42.698145630186445	15.706264593227958
16	17.800926418931617	20.895353968330475	41.4101864234079	19.893533189330004
17	18.462691591871994	21.47891494574467	29.477339652441575	30.58105380994176
18	22.15816677110084	24.430273281254493	33.19594354501524	20.215616402629426
19	28.56952831249694	25.31484381392857	25.965847969997686	20.149779903576807
20	29.874525795988312	23.4886095757357	27.119331830527436	19.517532797748558
21	23.29924557051511	27.661679607910393	27.99945597081373	21.039618850760764
22	22.98956741833117	25.469608160736712	25.963606091482845	25.57721832944928
23	21.07343385169301	28.921802156462945	26.680035735543527	23.32472825630052
24	20.75179901409656	23.881349326894657	37.326866261110844	18.03998539789794
25	18.988972573231898	26.196462539891424	34.08626823254408	20.7282966543326
26	18.675670050782283	32.44667038071207	28.038576750897782	20.839082817607864
27	19.129986731815656	32.78407309719627	28.75799556631159	19.327944604676482
28	17.44263686761745	28.61376804852322	35.23396057357714	18.70963451028219
29	17.83055657996949	25.09009549281534	34.98627036232867	22.093077564886503
30	19.47788891267771	30.568947665961595	30.08862519415602	19.864538227204672
31	27.301297636649036	27.749262328557013	26.262672685363185	18.68676734943077
32	27.992543512059626	26.692515525942834	27.221935137223515	18.09300582477403
33	27.061454000202517	27.895806454144058	24.8276462480108	20.21509329764263
34	20.422467060265806	28.049375132410947	27.991086291024974	23.53707151629827
35	22.379290721948404	25.867803149615128	29.957251113186096	21.79565501525038
36	28.441778601792684	27.029731419217455	25.80749661756579	18.72099336142407
37	21.02228165691263	32.634988175959066	27.895769089502142	18.446961077626163
38	21.204658474095282	32.37011022943011	25.335880107326197	21.089351189148413
39	21.913316272637836	31.420898866244674	27.462526066508314	19.20325879460918
40	24.42373446891953	27.123479305779902	25.913612200601793	22.539174024698774
41	18.825651723425427	25.783508517456948	27.99471066129064	27.396129097826982
42	21.38023492644957	25.952321969624787	26.076223122211893	26.591219981713742
43	21.865751083481204	26.482563603027582	27.427067021331847	24.224618292159366
44	20.14223224591016	29.574749273911593	28.69309318330682	21.58992529687142
45	18.817693054697727	35.005102141853364	24.439763900300672	21.737440903148233
46	21.48418336025456	33.23233670623956	25.940738930631426	19.34274100287446
47	21.36028220766745	28.27666424917433	27.934254670673653	22.428798872484563
48	22.3981225014731	25.653554292879754	30.502849614415577	21.445473591231565
49	21.37795568329281	26.27022034302983	30.916476200404507	21.43534777327285
50	20.13326473185078	32.35763043903081	27.543831527313365	19.96527330180505
51	19.625068237177295	32.3485881956876	25.64791223195073	22.37843133518438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1152.0
1	2515.0
2	3878.0
3	10878.0
4	17878.0
5	13593.5
6	9309.0
7	9537.5
8	9766.0
9	10454.0
10	11142.0
11	11547.5
12	11953.0
13	11754.5
14	11556.0
15	11122.5
16	10689.0
17	10498.5
18	10308.0
19	10288.0
20	10268.0
21	11989.0
22	13710.0
23	13411.5
24	13113.0
25	15257.0
26	22661.0
27	27921.0
28	32503.0
29	37085.0
30	42574.5
31	48064.0
32	55686.5
33	63309.0
34	74229.5
35	85150.0
36	89681.0
37	94212.0
38	106719.0
39	119226.0
40	146001.0
41	172776.0
42	205577.5
43	238379.0
44	251583.0
45	264787.0
46	247977.0
47	231167.0
48	219294.5
49	207422.0
50	199712.0
51	192002.0
52	179682.0
53	167362.0
54	148968.0
55	130574.0
56	118332.5
57	106091.0
58	96854.5
59	87618.0
60	79989.0
61	72360.0
62	64419.5
63	56479.0
64	48690.0
65	40901.0
66	34121.0
67	27341.0
68	22721.0
69	18101.0
70	15169.0
71	12237.0
72	11367.5
73	10498.0
74	8276.5
75	4596.0
76	3137.0
77	2442.5
78	1748.0
79	1520.5
80	1293.0
81	902.0
82	511.0
83	352.0
84	193.0
85	155.0
86	117.0
87	75.0
88	33.0
89	29.0
90	25.0
91	20.0
92	15.0
93	14.0
94	13.0
95	7.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2676327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.270983192705597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.86920279787972	12.995504563390956
2	6.998418747127971	2.2774230762006997
3	2.4419718984972847	1.191998511525261
4	1.3108023900022636	0.8531217462674063
5	0.8770455224649208	0.7135196477632213
6	0.5938581420508263	0.579759350889622
7	0.4384285488628131	0.4993564484824395
8	0.34112542938712465	0.4440356902529908
9	0.2978456377963607	0.43616172299447376
>10	3.575529814375427	14.38654370880967
>50	1.600737560453046	18.9540525835063
>100	1.645774582484114	44.76311573674618
>500	0.007175669679023612	0.7088170574188136
>1k	0.001620312508166622	0.5302945891847447
>5k	4.629464309047491E-4	0.6662955665672812
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9782	0.3655009272035891	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7909	0.29551695289850605	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3885	0.14516163383622405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.736464191408598E-5	0.0	0.0	0.022045138729310732	0.0
2	3.736464191408598E-5	0.0	0.0	0.07394462634797616	0.0
3	3.736464191408598E-5	0.0	0.0	0.10701233444194226	0.0
4	3.736464191408598E-5	0.0	0.0	0.1690002753774109	0.0
5	3.736464191408598E-5	0.0	0.0	0.2853537702978747	0.0
6	3.736464191408598E-5	0.0	0.0	0.438474072861799	0.0
7	3.736464191408598E-5	0.0	0.0	0.5278502963202927	0.0
8	3.736464191408598E-5	0.0	0.0	0.7654146896100514	0.0
9	3.736464191408598E-5	0.0	0.0	0.8768360517978558	0.0
10	7.472928382817197E-5	0.0	0.0	1.0626130513946912	0.0
11	7.472928382817197E-5	0.0	0.0	1.2263822769041302	0.0
12	7.472928382817197E-5	0.0	0.0	1.3664623194400385	0.0
13	7.472928382817197E-5	0.0	0.0	1.425834735441521	0.0
14	7.472928382817197E-5	0.0	0.0	1.4471699459744642	0.0
15	7.472928382817197E-5	0.0	0.0	1.4802376540684303	0.0
16	7.472928382817197E-5	0.0	0.0	1.5541075511325784	0.0
17	7.472928382817197E-5	0.0	0.0	1.643521139232986	0.0
18	7.472928382817197E-5	0.0	0.0	1.7645825790346248	0.0
19	7.472928382817197E-5	0.0	0.0	1.8262716028347807	0.0
20	7.472928382817197E-5	0.0	0.0	1.8902772344336098	0.0
21	7.472928382817197E-5	0.0	0.0	1.979317176114877	0.0
22	7.472928382817197E-5	0.0	0.0	2.071906758777982	0.0
23	7.472928382817197E-5	0.0	0.0	2.1777234246786734	0.0
24	7.472928382817197E-5	0.0	0.0	2.2632884546619305	0.0
25	7.472928382817197E-5	0.0	0.0	2.3359253185429134	0.0
26	7.472928382817197E-5	0.0	0.0	2.406469762476708	0.0
27	7.472928382817197E-5	0.0	0.0	2.4754075268081963	0.0
28	7.472928382817197E-5	0.0	0.0	2.5489411420951176	0.0
29	7.472928382817197E-5	0.0	0.0	2.6283036415206364	0.0
30	1.4945856765634393E-4	0.0	0.0	2.7310190421424587	0.0
31	1.868232095704299E-4	0.0	0.0	2.8236459894474777	0.0
32	2.241878514845159E-4	0.0	0.0	2.9170575942326926	0.0
33	2.241878514845159E-4	0.0	0.0	3.0038556573991144	0.0
34	2.241878514845159E-4	0.0	0.0	3.1029093231133564	0.0
35	2.241878514845159E-4	0.0	0.0	3.2193749119595623	0.0
36	2.241878514845159E-4	0.0	0.0	3.3222397711490412	0.0
37	2.241878514845159E-4	0.0	0.0	3.428616906678444	0.0
38	2.241878514845159E-4	0.0	0.0	3.536376533958668	0.0
39	2.241878514845159E-4	0.0	0.0	3.6477978961464723	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	85	0.0	45.000004	1
CTCGATT	60	0.0	45.000004	20
TTACACG	100	0.0	42.75	34
ACTCGAT	60	3.6379788E-12	41.250004	19
CGTAGTA	45	1.9299478E-8	40.0	23
ACTCGAC	45	1.9299478E-8	40.0	20
GCTACGA	520	0.0	39.80769	10
CGTTTTT	8350	0.0	39.74551	1
CGTTAGG	385	0.0	39.740257	2
TACGTTC	40	3.4605182E-7	39.375	27
ACGTTAG	145	0.0	38.793106	1
CGAGGGT	530	0.0	38.632076	4
ATTACGC	35	6.2514027E-6	38.571426	44
CATTACG	35	6.2514027E-6	38.571426	43
CTACGTT	35	6.2514027E-6	38.571426	45
GGGCGAT	5125	0.0	38.107315	7
TAGGGTA	1785	0.0	37.815125	5
AGGGCGA	3045	0.0	37.75862	6
AAACTCG	30	1.140327E-4	37.500004	1
CGGCTAC	1185	0.0	37.40506	25
>>END_MODULE