FastQCFastQC Report
Thu 26 May 2016
SRR1545410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545410_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6549934
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1044521.5947030916647404No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA172450.2632850956971475No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC78800.12030655576071453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT707100.043.246711
TGCATTA25150.038.64811315
GGGCGAT130000.038.4403847
CGTTAGG7250.038.1724172
GTTTTTT840050.037.7682882
ATACTAT50450.037.2398445
GGCGATA27500.037.2272728
CTCAACT27700.037.1209372
AGGGATC55300.036.9439436
GCATTAG26450.036.83364516
CGAATAT8600.036.62790714
TGATTCG10000.036.44999715
TCAACTT28050.036.417113
AGGGCGA68950.036.2871676
ATTATAC52400.036.28339842
CTTGACC28200.035.9840437
TATACTA52450.035.9485244
CTACGAA9050.035.80110511
TACGGGA13150.035.7604564
GACCGAT33200.035.715369
Produced by FastQC (version 0.11.5)