##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545410_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	6549934
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226632665306244	31.0	31.0	33.0	30.0	34.0
2	31.60561220922226	31.0	31.0	34.0	30.0	34.0
3	31.611447077176656	31.0	31.0	34.0	30.0	34.0
4	35.40577324901289	37.0	35.0	37.0	33.0	37.0
5	35.29632619198911	37.0	35.0	37.0	33.0	37.0
6	35.371019463707576	37.0	35.0	37.0	33.0	37.0
7	35.792674246793936	37.0	35.0	37.0	35.0	37.0
8	35.81186161570483	37.0	35.0	37.0	35.0	37.0
9	37.465895534214546	39.0	37.0	39.0	35.0	39.0
10	36.889657056086364	39.0	37.0	39.0	32.0	39.0
11	36.59096580209816	39.0	35.0	39.0	32.0	39.0
12	36.23872026802102	38.0	35.0	39.0	32.0	39.0
13	36.078737587279505	38.0	35.0	39.0	31.0	39.0
14	37.13336790874534	39.0	35.0	41.0	31.0	41.0
15	37.2254071567744	39.0	35.0	41.0	32.0	41.0
16	37.294578540791406	39.0	35.0	41.0	32.0	41.0
17	37.21944877612507	39.0	35.0	41.0	32.0	41.0
18	37.002647965613086	39.0	35.0	40.0	31.0	41.0
19	36.82303348400152	38.0	35.0	40.0	31.0	41.0
20	36.54124835456357	38.0	35.0	40.0	31.0	41.0
21	36.358438420906225	38.0	35.0	40.0	30.0	41.0
22	36.34490378070985	38.0	35.0	40.0	30.0	41.0
23	36.23373975982048	38.0	35.0	40.0	30.0	41.0
24	36.13230850875749	38.0	35.0	40.0	30.0	41.0
25	36.00073084705892	38.0	35.0	40.0	30.0	41.0
26	35.942211936792035	38.0	35.0	40.0	30.0	41.0
27	35.90053838710436	38.0	35.0	40.0	30.0	41.0
28	35.82080506460065	38.0	35.0	40.0	30.0	41.0
29	35.7468298153844	37.0	34.0	40.0	29.0	41.0
30	35.577859257818474	37.0	34.0	40.0	29.0	41.0
31	35.33372305736211	37.0	34.0	40.0	27.0	41.0
32	34.996436757988704	37.0	34.0	40.0	25.0	41.0
33	34.581361430512125	37.0	33.0	40.0	23.0	41.0
34	34.14873676589718	37.0	33.0	40.0	21.0	41.0
35	33.838177148044544	37.0	33.0	40.0	20.0	41.0
36	33.638516815589284	37.0	33.0	40.0	18.0	41.0
37	33.54428930123571	37.0	33.0	40.0	18.0	41.0
38	33.43100877047005	36.0	32.0	40.0	18.0	41.0
39	33.27805348878324	36.0	32.0	40.0	18.0	41.0
40	33.116975529829766	36.0	32.0	40.0	17.0	41.0
41	32.982620588237985	36.0	32.0	40.0	16.0	41.0
42	32.84842198409938	35.0	31.0	40.0	15.0	41.0
43	32.76929950744542	35.0	31.0	40.0	15.0	41.0
44	32.65391788680619	35.0	31.0	40.0	15.0	41.0
45	32.610234393201516	35.0	31.0	40.0	15.0	41.0
46	32.55991831368072	35.0	31.0	40.0	15.0	41.0
47	32.50032534678975	35.0	31.0	39.0	15.0	41.0
48	32.43234847862589	35.0	31.0	39.0	15.0	41.0
49	32.34045671299894	35.0	31.0	39.0	15.0	41.0
50	32.23438587320116	35.0	31.0	39.0	15.0	41.0
51	32.07799055685141	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	5.0
12	8.0
13	24.0
14	82.0
15	269.0
16	891.0
17	2365.0
18	5686.0
19	10935.0
20	18505.0
21	28857.0
22	42434.0
23	61325.0
24	86918.0
25	128440.0
26	173226.0
27	192045.0
28	188297.0
29	187992.0
30	203191.0
31	231437.0
32	271998.0
33	336426.0
34	493183.0
35	648373.0
36	532640.0
37	650711.0
38	864214.0
39	1188985.0
40	466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.779475640517905	22.292377297236886	26.44078551020514	21.487361552040067
2	37.51340089839073	23.38331958764775	26.0687512271116	13.034528286849914
3	26.757185644924053	23.544359378277704	36.8521270596009	12.846327917197334
4	24.08016630396581	25.34211795111218	36.05306557287447	14.524650172047535
5	21.138716817604575	29.443273779552587	35.49060189003431	13.927407512808527
6	20.382022169994386	37.10854185706299	32.470769934475676	10.038666038466951
7	78.29242248853194	4.4843352620041665	14.559032197881688	2.664210051582199
8	78.97728435126217	3.857641924330841	14.948272761221714	2.216800963185278
9	74.108349794059	5.49480345908829	16.827925289018182	3.568921457834537
10	40.738013543342575	23.17482893720761	23.010888353989518	13.076269165460294
11	31.284910657115017	23.366632396601247	29.55165655104311	15.796800395240624
12	26.994684221245592	20.69211689766645	34.28481874779196	18.028380133296
13	22.041916758245197	25.11519963407265	34.39109462782373	18.45178897985842
14	19.324866479570634	26.197943368589666	35.598908324877776	18.87828182696192
15	18.36392549909663	21.598461908165792	41.572266224361954	18.46534636837562
16	21.50597242659239	19.76648925012069	38.93207168194366	19.795466641343257
17	21.161694148368518	20.101836140638973	33.91893719845116	24.817532512541348
18	23.50684754991424	20.923096324329375	35.9242703819611	19.64578574379528
19	26.217226005636086	22.668778036542047	31.914107836811787	19.199888121010076
20	27.55482727001524	21.4541856452294	32.47751809407545	18.513468990679907
21	24.93538102826685	23.345227600766663	32.94117162096595	18.778219750000535
22	25.34552256557089	21.3100315209283	31.94479516892842	21.399650744572387
23	22.569173979463	24.554461159455958	32.10223186981732	20.77413299126373
24	21.34821511178586	22.453004869972737	37.14211471443834	19.05666530380306
25	21.167114050309515	23.13986675285583	34.786152043669446	20.906867153165205
26	20.294082352585537	24.18193832182126	33.614934135214185	21.909045190379018
27	19.568655195609605	23.915553958253625	34.538836574536475	21.976954271600295
28	19.495860568976724	23.39003721258871	36.311373519183554	20.802728699251016
29	20.128370759155743	22.735343592775134	36.15172305553003	20.9845625925391
30	20.783995686063403	23.528481355689994	34.9561384893344	20.731384468912207
31	24.151205187716396	23.293196542133096	32.196614500237715	20.358983769912797
32	24.860830658751677	22.892139065828754	31.464668193603174	20.782362081816398
33	24.53881214680942	23.90891266995973	30.175296422834187	21.37697876039667
34	21.229389486978036	25.132863934201477	31.702701126454098	21.93504545236639
35	22.260392242120304	26.492648628215186	30.282854758536498	20.964104371128016
36	24.432917950012932	27.121952679217838	27.430658079913478	21.014471290855756
37	21.878434194909442	29.27614232448754	27.731882489197602	21.11354099140541
38	22.900475027687303	28.153230246289507	27.632186828142085	21.314107897881108
39	22.599418559026702	27.870357166957717	27.705943296527874	21.82428097748771
40	24.9648775086894	24.935854315478597	28.567600833840462	21.531667341991536
41	21.89609849503827	24.800020885706633	28.921818143511064	24.38206247574403
42	22.386164501810246	24.772646564072247	28.138466738748818	24.702722195368686
43	22.24266687267383	25.041336294380983	28.986963838108903	23.72903299483628
44	21.992236868340964	26.069178712335116	29.21884403720709	22.719740382116825
45	20.72466684397125	27.95683132074308	27.63913346302421	23.67936837226146
46	21.497743336039722	26.987157427845837	28.463966201796843	23.051133034317598
47	21.61383610888293	26.683016958644163	29.496678897833167	22.20646803463974
48	22.424683973914853	25.939299541033545	29.82065162794007	21.81536485711154
49	22.080085081773344	25.509875366683083	30.019218514262892	22.39082103728068
50	21.083662827747577	27.94347240750823	29.184660486655282	21.78820427808891
51	21.30998571894007	27.087540118724863	28.682273745048427	22.920200417286647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4154.0
1	7488.0
2	10822.0
3	88780.0
4	166738.0
5	116831.0
6	66924.0
7	68019.0
8	69114.0
9	70885.0
10	72656.0
11	73184.5
12	73713.0
13	72152.0
14	70591.0
15	67004.0
16	63417.0
17	60005.0
18	56593.0
19	52523.0
20	48453.0
21	48280.0
22	48107.0
23	47146.5
24	46186.0
25	51198.5
26	58949.0
27	61687.0
28	71651.5
29	81616.0
30	96908.5
31	112201.0
32	116696.0
33	121191.0
34	138081.0
35	154971.0
36	172403.0
37	189835.0
38	201469.5
39	213104.0
40	243744.5
41	274385.0
42	314024.5
43	353664.0
44	366515.0
45	379366.0
46	392611.5
47	405857.0
48	399395.0
49	392933.0
50	390902.5
51	388872.0
52	374368.0
53	359864.0
54	351667.5
55	343471.0
56	343130.0
57	342789.0
58	339039.0
59	335289.0
60	317997.5
61	300706.0
62	279330.5
63	257955.0
64	233406.5
65	208858.0
66	180696.5
67	152535.0
68	125894.5
69	99254.0
70	80992.0
71	62730.0
72	53403.5
73	44077.0
74	36658.0
75	21947.5
76	14656.0
77	11056.5
78	7457.0
79	5894.5
80	4332.0
81	2970.5
82	1609.0
83	1268.0
84	927.0
85	717.0
86	507.0
87	326.0
88	145.0
89	130.0
90	115.0
91	72.0
92	29.0
93	22.0
94	15.0
95	14.5
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6549934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.029332197048976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.71739720586001	16.36744894914372
2	6.188972987099257	2.47921991834347
3	2.0261001649434163	1.2174429980444224
4	1.1587608931063709	0.9283682745990667
5	0.7503187263809293	0.7514191512175165
6	0.5990331321009825	0.719894015993358
7	0.48690328350828316	0.6826643329254926
8	0.40127296226249937	0.6429783570279598
9	0.3365214734495898	0.6066270344846009
>10	3.800215183253017	18.20529975013183
>50	1.4862077842617485	21.36626196313958
>100	1.0391616578080785	32.3170081840391
>500	0.006579969660542428	0.8506966632776041
>1k	0.0022449306928207713	0.7718537630973241
>5k	1.548228064014319E-4	0.20590622876062037
>10k+	1.548228064014319E-4	1.886910415774188
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104452	1.5947030916647404	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	17245	0.2632850956971475	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7880	0.12030655576071453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.526732941125819E-5	0.0	0.0	0.016748260364150235	0.0
2	1.526732941125819E-5	0.0	0.0	0.05468757395112683	0.0
3	1.526732941125819E-5	0.0	0.0	0.09974146304374976	0.0
4	1.526732941125819E-5	0.0	0.0	0.15412369040665141	0.0
5	1.526732941125819E-5	0.0	0.0	0.2936212792373175	0.0
6	1.526732941125819E-5	0.0	0.0	0.57334928871039	0.0
7	1.526732941125819E-5	0.0	0.0	0.7765269085154141	0.0
8	3.053465882251638E-5	0.0	0.0	1.1381794076092981	0.0
9	3.053465882251638E-5	0.0	0.0	1.3843650943658363	0.0
10	3.053465882251638E-5	0.0	0.0	1.6826887110618214	0.0
11	3.053465882251638E-5	0.0	0.0	1.8902327870784652	0.0
12	6.106931764503276E-5	0.0	0.0	2.047730557285005	0.0
13	6.106931764503276E-5	0.0	0.0	2.1328001167645354	0.0
14	9.160397646754914E-5	0.0	0.0	2.185121254656917	0.0
15	1.0687130587880732E-4	0.0	0.0	2.2342209860435234	0.0
16	1.0687130587880732E-4	0.0	0.0	2.2887253520417152	0.0
17	1.0687130587880732E-4	0.0	0.0	2.353229818804281	0.0
18	1.0687130587880732E-4	0.0	0.0	2.451322410271615	0.0
19	1.0687130587880732E-4	0.0	0.0	2.5053840237168803	0.0
20	1.0687130587880732E-4	0.0	0.0	2.5659953214795754	0.0
21	1.0687130587880732E-4	0.0	0.0	2.632560877712661	0.0
22	1.0687130587880732E-4	0.0	0.0	2.7113250301453418	0.0
23	1.0687130587880732E-4	0.0	0.0	2.7984556790953925	0.0
24	1.2213863529006552E-4	0.0	0.0	2.870028919375371	0.0
25	1.526732941125819E-4	0.0	0.0	2.939693743478942	0.0
26	1.6794062352384008E-4	0.0	0.0	3.0026104079827367	1.526732941125819E-5
27	1.8320795293509827E-4	0.0	0.0	3.071985152827494	1.526732941125819E-5
28	1.9847528234635646E-4	0.0	0.0	3.139268273542909	1.526732941125819E-5
29	2.1374261175761465E-4	0.0	0.0	3.2185362478461617	1.526732941125819E-5
30	2.1374261175761465E-4	0.0	0.0	3.3311175349247795	1.526732941125819E-5
31	2.2900994116887286E-4	0.0	0.0	3.4190420850042154	1.526732941125819E-5
32	2.2900994116887286E-4	0.0	0.0	3.522737786365481	1.526732941125819E-5
33	2.4427727058013105E-4	0.0	0.0	3.6111508909860772	1.526732941125819E-5
34	2.4427727058013105E-4	0.0	0.0	3.712190077029784	1.526732941125819E-5
35	2.595445999913892E-4	0.0	0.0	3.8264660376730513	1.526732941125819E-5
36	2.595445999913892E-4	0.0	0.0	3.9229250248933805	1.526732941125819E-5
37	2.595445999913892E-4	0.0	0.0	4.024956587348819	1.526732941125819E-5
38	2.595445999913892E-4	0.0	0.0	4.147537975191811	1.526732941125819E-5
39	2.748119294026474E-4	0.0	0.0	4.345387297032306	1.526732941125819E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	70710	0.0	43.24671	1
TGCATTA	2515	0.0	38.648113	15
GGGCGAT	13000	0.0	38.440384	7
CGTTAGG	725	0.0	38.172417	2
GTTTTTT	84005	0.0	37.768288	2
ATACTAT	5045	0.0	37.23984	45
GGCGATA	2750	0.0	37.227272	8
CTCAACT	2770	0.0	37.120937	2
AGGGATC	5530	0.0	36.943943	6
GCATTAG	2645	0.0	36.833645	16
CGAATAT	860	0.0	36.627907	14
TGATTCG	1000	0.0	36.449997	15
TCAACTT	2805	0.0	36.41711	3
AGGGCGA	6895	0.0	36.287167	6
ATTATAC	5240	0.0	36.283398	42
CTTGACC	2820	0.0	35.984043	7
TATACTA	5245	0.0	35.94852	44
CTACGAA	905	0.0	35.801105	11
TACGGGA	1315	0.0	35.760456	4
GACCGAT	3320	0.0	35.71536	9
>>END_MODULE