FastQCFastQC Report
Thu 26 May 2016
SRR1545409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545409_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1079871
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34520.3196678121738615No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.24688134045640636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA253.890648E-545.0000049
GTCCTAT207.033095E-445.0000049
CCGCTAT207.033095E-445.00000437
CGTTAGA302.1653796E-645.00000414
TGTTGCG253.890648E-545.0000041
CTAACCG253.890648E-545.0000041
CGGATGA253.890648E-545.0000046
TACGACT207.033095E-445.00000440
ACGTGCG253.890648E-545.0000041
CTCGCTA207.033095E-445.00000436
CGTCGGT207.033095E-445.00000423
TTACGCG207.033095E-445.0000041
CCGAACT207.033095E-445.00000421
GCTACGT207.033095E-445.00000429
CAATCGA207.033095E-445.00000443
TGTAACG502.1827873E-1145.0000041
TGTCGGT207.033095E-445.00000411
CAATACG207.033095E-445.0000041
CGTATTA551.8189894E-1245.035
TATAGCG700.045.01