FastQCFastQC Report
Thu 26 May 2016
SRR1545408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545408_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1456371
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.3759344287959593No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41650.2859848211753736No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17360.11920039605292881No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16850.11569854109976098No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA253.891321E-545.016
CGTATTG207.033908E-445.035
CTATTCG207.033908E-445.044
ACCAACG207.033908E-445.01
ACGTTAC302.1659034E-644.99999620
CCGACGG451.9288564E-840.02
TTCGAAG900.040.01
TTATACG403.459063E-739.3751
CGACTCG403.459063E-739.3753
CAATCGA403.459063E-739.37517
TAGTCCC403.459063E-739.37528
TCGAACG403.459063E-739.3751
TTACGGG4700.039.2553183
TAGTGCG1150.039.1304321
CGAATAT2150.038.72093214
CGGCTAC6050.038.6776925
CGTTTTT46250.038.578381
CGTTACA356.2494382E-638.57142621
GACGGGA3400.038.382354
GCGGCTA6100.038.36065724