##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545408_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1456371
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254912381529156	31.0	31.0	33.0	30.0	34.0
2	31.59601639966739	31.0	31.0	34.0	30.0	34.0
3	31.699527798891904	31.0	31.0	34.0	30.0	34.0
4	35.40252861393148	37.0	35.0	37.0	33.0	37.0
5	35.261423771827374	37.0	35.0	37.0	33.0	37.0
6	35.32917367895955	37.0	35.0	37.0	32.0	37.0
7	35.798232730533634	37.0	35.0	37.0	35.0	37.0
8	35.81971558071398	37.0	35.0	37.0	35.0	37.0
9	37.44275256785531	39.0	37.0	39.0	35.0	39.0
10	36.8081615192832	39.0	37.0	39.0	32.0	39.0
11	36.46829619650487	39.0	35.0	39.0	32.0	39.0
12	35.770449287990495	37.0	35.0	39.0	31.0	39.0
13	35.45245133279913	37.0	35.0	39.0	30.0	39.0
14	36.439427865564475	38.0	35.0	40.0	31.0	41.0
15	36.647512893349294	38.0	35.0	41.0	31.0	41.0
16	36.8069516627288	38.0	35.0	41.0	32.0	41.0
17	36.77106726239399	38.0	35.0	41.0	32.0	41.0
18	36.693699613628674	38.0	35.0	40.0	31.0	41.0
19	36.65352097782777	38.0	35.0	40.0	31.0	41.0
20	36.511387551660945	38.0	35.0	40.0	31.0	41.0
21	36.313329501892035	38.0	35.0	40.0	31.0	41.0
22	36.23954953785814	38.0	35.0	40.0	30.0	41.0
23	36.1722109270234	38.0	35.0	40.0	30.0	41.0
24	36.0780007292098	37.0	34.0	40.0	30.0	41.0
25	35.943004220765175	37.0	34.0	40.0	30.0	41.0
26	35.8669604104998	37.0	34.0	40.0	30.0	41.0
27	35.82067756086876	37.0	34.0	40.0	30.0	41.0
28	35.78917322577832	37.0	34.0	40.0	30.0	41.0
29	35.81192841659165	37.0	34.0	40.0	30.0	41.0
30	35.73802417103884	37.0	34.0	40.0	30.0	41.0
31	35.58781313278004	37.0	34.0	40.0	29.0	41.0
32	35.41404765681272	37.0	34.0	40.0	29.0	41.0
33	35.26893010091522	37.0	34.0	40.0	28.0	41.0
34	35.10533854354419	37.0	34.0	40.0	27.0	41.0
35	34.98109616299693	37.0	34.0	40.0	27.0	41.0
36	34.82159284962417	37.0	34.0	40.0	25.0	41.0
37	34.762248081017816	37.0	33.0	40.0	25.0	41.0
38	34.72563378424866	36.0	33.0	40.0	26.0	41.0
39	34.65118640785899	36.0	33.0	40.0	25.0	41.0
40	34.47148631770339	36.0	33.0	40.0	24.0	41.0
41	34.42617368788585	36.0	33.0	40.0	24.0	41.0
42	34.35804200989995	36.0	33.0	40.0	24.0	41.0
43	34.29601111255305	36.0	33.0	40.0	24.0	41.0
44	34.23798674925551	35.0	33.0	40.0	24.0	41.0
45	34.202960646703346	35.0	33.0	40.0	24.0	41.0
46	34.161355176668586	35.0	33.0	40.0	24.0	41.0
47	34.102120956816634	35.0	33.0	40.0	23.0	41.0
48	34.03616180217816	35.0	33.0	40.0	23.0	41.0
49	33.99950424720075	36.0	33.0	40.0	24.0	41.0
50	33.85907368383468	35.0	33.0	39.0	24.0	41.0
51	33.697544787694895	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	8.0
13	6.0
14	18.0
15	35.0
16	162.0
17	371.0
18	837.0
19	1704.0
20	2837.0
21	4613.0
22	6727.0
23	9538.0
24	13235.0
25	18196.0
26	23613.0
27	27751.0
28	31625.0
29	36884.0
30	43246.0
31	52555.0
32	63960.0
33	81353.0
34	132889.0
35	197838.0
36	107270.0
37	128979.0
38	179452.0
39	290513.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.76255088847554	23.881277504152447	28.375393357873786	13.980778249498238
2	31.41266888725469	26.003813588707825	29.022755877451555	13.56076164658593
3	29.523452471931943	25.062569908354398	31.282962926342258	14.1310146933714
4	26.607643244750136	27.976525212325704	30.538166442479287	14.877665100444872
5	23.37371452741094	32.37890619903857	29.28621896481048	14.961160308740013
6	21.854184133026543	40.915879264280875	26.87975797375806	10.350178628934522
7	84.91545080202778	5.532724834537354	7.317022928910284	2.234801434524582
8	85.75960383720907	4.549596222391136	7.186836321239574	2.5039636191602277
9	80.09051265096599	6.553618549119696	9.477804762660064	3.8780640372542434
10	47.92734818257161	23.43722856332624	16.023595636002092	12.611827618100058
11	42.43080918255033	22.714061183585777	20.966498234309803	13.888631399554097
12	37.07873886530287	22.316222995376865	25.603846821997966	15.001191317322304
13	22.34258990325954	36.145322860727106	26.00607949485399	15.506007741159362
14	17.2817228577059	38.58906830745737	27.743823517496573	16.385385317340155
15	16.369043327558703	24.139110158057253	43.987212049676906	15.504634464707138
16	17.41547998415239	20.234679212920334	43.12397047180973	19.22587033111755
17	17.8435302543102	20.937247445877457	29.334626959751326	31.88459534006102
18	21.331927098246258	24.536605027153108	33.3041512087236	20.82731666587703
19	29.109821604522473	24.741223218534287	26.19785755140689	19.951097625536352
20	31.251240240295914	22.552563872804388	25.945380675665746	20.25081521123395
21	23.51042419822971	27.299499921379923	27.55170214183062	21.63837373855975
22	23.02112579830277	26.00539285662788	25.310240316512754	25.663241028556598
23	21.246577966740617	29.65439438165138	25.906654279713067	23.192373371894938
24	20.93862072232968	23.817626140591923	36.573785113820584	18.66996802325781
25	19.018024940073648	25.626986530217916	33.81658931687049	21.538399212837938
26	18.151830817834192	33.06149325961585	28.612970184108306	20.173705738441647
27	20.041047233156934	32.44132161379209	28.58042353219063	18.937207620860345
28	17.060282029784993	29.303659575753706	35.20195060187274	18.434107792588563
29	18.47743466465619	24.741223218534287	35.156701142772	21.624640974037522
30	19.70521247676588	29.853588131046276	30.021196522040057	20.420002870147787
31	26.80093190540048	27.636021315997088	25.9999684146416	19.56307836396083
32	28.118247342195087	26.150410849982592	27.69108970173122	18.0402521060911
33	26.72553902817345	27.913766478459127	25.6361188186252	19.72457567474222
34	20.199797991033876	27.383750431723787	28.17537564260755	24.241075934634786
35	21.243900077658783	25.577067931179627	30.126664153570758	23.052367837590833
36	27.644947612936537	26.602287466586468	26.530121789022164	19.222643131454827
37	20.618715972784408	32.438437733242424	28.84642718098616	18.096419112987007
38	21.17654086767726	31.372225895736733	25.33894179436421	22.112291442221796
39	21.733129813763114	30.978507536884486	27.036929463714948	20.251433185637453
40	24.109996697270134	26.42266290663574	26.980213146238153	22.48712724985598
41	18.26546944425562	25.860718182386215	28.22261635256401	27.65119602079415
42	21.316546401981363	26.425409459540184	25.95348300673386	26.30456113174459
43	22.301528937338084	26.917729067662016	26.771887108435973	24.00885488656393
44	20.568522718455668	29.817676951820655	28.131018813200757	21.48278151652292
45	18.62464990033446	34.4684836487406	24.230501705952673	22.676364744972265
46	21.431008994274123	32.833117385611224	25.738427914315785	19.997445705798867
47	22.650341156202643	27.470472839681648	26.968402968749032	22.910783035366673
48	22.853792062599435	24.510444110738266	30.466550075495874	22.169213751166428
49	20.917540928788064	25.860237535627938	30.78851473971948	22.433706795864516
50	19.521468087458484	32.37629697377935	27.106417252197414	20.995817686564756
51	19.570356729157613	32.06284662355952	25.276869698723747	23.089926948559125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	749.0
1	1458.0
2	2167.0
3	6017.5
4	9868.0
5	7493.5
6	5119.0
7	5250.5
8	5382.0
9	5750.5
10	6119.0
11	6416.0
12	6713.0
13	6629.0
14	6545.0
15	6761.5
16	6978.0
17	6484.5
18	5991.0
19	5962.5
20	5934.0
21	6372.0
22	6810.0
23	6828.5
24	6847.0
25	8270.0
26	11005.0
27	12317.0
28	16457.0
29	20597.0
30	23319.0
31	26041.0
32	29439.0
33	32837.0
34	37034.0
35	41231.0
36	42939.5
37	44648.0
38	53681.5
39	62715.0
40	79430.0
41	96145.0
42	113538.5
43	130932.0
44	137656.0
45	144380.0
46	138933.0
47	133486.0
48	122766.0
49	112046.0
50	105742.5
51	99439.0
52	90890.5
53	82342.0
54	75042.5
55	67743.0
56	63921.5
57	60100.0
58	55881.0
59	51662.0
60	49007.0
61	46352.0
62	40748.5
63	35145.0
64	29554.0
65	23963.0
66	20364.5
67	16766.0
68	14047.5
69	11329.0
70	9314.5
71	7300.0
72	6190.5
73	5081.0
74	4215.5
75	2516.5
76	1683.0
77	1326.5
78	970.0
79	744.0
80	518.0
81	351.5
82	185.0
83	125.5
84	66.0
85	42.0
86	18.0
87	23.5
88	29.0
89	17.5
90	6.0
91	16.5
92	27.0
93	14.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1456371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.936763497729938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39082389593047	15.628593164053624
2	6.856338263369604	2.7338638883447244
3	2.531956295844195	1.5143704157050217
4	1.4365046550290665	1.1455701428281053
5	0.9154610117646093	0.912566484147179
6	0.6569543668382816	0.7858526304273442
7	0.5021196343793857	0.7007448278731908
8	0.42411653153243456	0.6764408787711735
9	0.3441469231236004	0.6175058233308001
>10	5.231091436905739	25.89728962911066
>50	1.9130805929097674	26.903902110351787
>100	0.7939318547154801	21.267385518231595
>500	0.001737268828698997	0.24175591403285646
>1k	0.0013898150629591975	0.5948996532132296
>5k	3.474537657397994E-4	0.3792589195787648
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5475	0.3759344287959593	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4165	0.2859848211753736	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1736	0.11920039605292881	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1685	0.11569854109976098	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.046485407907737794	0.0
2	0.0	0.0	0.0	0.14865717595310535	0.0
3	0.0	0.0	0.0	0.21354448832062708	0.0
4	0.0	0.0	0.0	0.3381006625372244	0.0
5	0.0	0.0	0.0	0.5907148659235868	0.0
6	0.0	0.0	0.0	0.8955822383170222	0.0
7	0.0	0.0	0.0	1.07465748768686	0.0
8	0.0	0.0	0.0	1.5281133722107898	0.0
9	0.0	0.0	0.0	1.7186554799566869	0.0
10	0.0	0.0	0.0	2.040894799470739	0.0
11	0.0	0.0	0.0	2.30126801481216	0.0
12	0.0	0.0	0.0	2.5196189707155665	0.0
13	6.866382261113411E-5	0.0	0.0	2.6102552165622632	0.0
14	6.866382261113411E-5	0.0	0.0	2.645823076674831	0.0
15	6.866382261113411E-5	0.0	0.0	2.702196075038572	0.0
16	6.866382261113411E-5	0.0	0.0	2.8141181058947207	0.0
17	6.866382261113411E-5	0.0	0.0	2.9451286794367646	0.0
18	6.866382261113411E-5	0.0	0.0	3.1284610858084925	0.0
19	6.866382261113411E-5	0.0	0.0	3.2113383197001313	0.0
20	1.3732764522226821E-4	0.0	0.0	3.298335382948438	0.0
21	1.3732764522226821E-4	0.0	0.0	3.422136255116313	0.0
22	1.3732764522226821E-4	0.0	0.0	3.55815928770897	0.0
23	1.3732764522226821E-4	0.0	0.0	3.7156054329563	0.0
24	1.3732764522226821E-4	0.0	0.0	3.833707207847451	0.0
25	1.3732764522226821E-4	0.0	0.0	3.929836559503039	0.0
26	1.3732764522226821E-4	0.0	0.0	4.025004617642071	0.0
27	1.3732764522226821E-4	0.0	0.0	4.114473578504378	0.0
28	1.3732764522226821E-4	0.0	0.0	4.216508018904523	0.0
29	1.3732764522226821E-4	0.0	0.0	4.32286828012917	0.0
30	1.3732764522226821E-4	0.0	0.0	4.4657576949829405	0.0
31	1.3732764522226821E-4	0.0	0.0	4.600407451123375	0.0
32	1.3732764522226821E-4	0.0	0.0	4.72125577891897	0.0
33	1.3732764522226821E-4	0.0	0.0	4.8361303541473974	0.0
34	1.3732764522226821E-4	0.0	0.0	4.958763941330884	0.0
35	1.3732764522226821E-4	0.0	0.0	5.114424827190325	0.0
36	1.3732764522226821E-4	0.0	0.0	5.245160745441924	0.0
37	1.3732764522226821E-4	0.0	0.0	5.374798042531745	0.0
38	1.3732764522226821E-4	0.0	0.0	5.502169433475399	0.0
39	1.3732764522226821E-4	0.0	0.0	5.635583240808832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	3.891321E-5	45.0	16
CGTATTG	20	7.033908E-4	45.0	35
CTATTCG	20	7.033908E-4	45.0	44
ACCAACG	20	7.033908E-4	45.0	1
ACGTTAC	30	2.1659034E-6	44.999996	20
CCGACGG	45	1.9288564E-8	40.0	2
TTCGAAG	90	0.0	40.0	1
TTATACG	40	3.459063E-7	39.375	1
CGACTCG	40	3.459063E-7	39.375	3
CAATCGA	40	3.459063E-7	39.375	17
TAGTCCC	40	3.459063E-7	39.375	28
TCGAACG	40	3.459063E-7	39.375	1
TTACGGG	470	0.0	39.255318	3
TAGTGCG	115	0.0	39.130432	1
CGAATAT	215	0.0	38.720932	14
CGGCTAC	605	0.0	38.67769	25
CGTTTTT	4625	0.0	38.57838	1
CGTTACA	35	6.2494382E-6	38.571426	21
GACGGGA	340	0.0	38.38235	4
GCGGCTA	610	0.0	38.360657	24
>>END_MODULE