FastQCFastQC Report
Thu 26 May 2016
SRR1545407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545407_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2681201
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101610.3789719606997014No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC84750.31608969264146924No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT39770.1483290510483921No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28650.1068550996363197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC207.0349703E-445.0000047
CACTACG207.0349703E-445.0000041
CGTACGT253.8922008E-545.00000439
TATCGTG800.045.0000041
GGTCGAA551.8189894E-1244.9999969
TATCGAT603.6379788E-1241.24999631
TGCGTAT556.184564E-1140.9090936
GCGCGAC3650.040.6849339
GTGCAAC451.9299478E-840.045
CGTTTTT85100.039.84431
CGTATTA403.4605182E-739.37500430
TAGCCCG403.4605182E-739.37500429
TGTTGCG2000.039.3750041
TAATGCG800.039.3750041
CTTAACG1200.039.3749961
ATTATAC24800.038.64919342
GGCCTAT9500.038.6052638
ATACGTA356.2514064E-638.57142643
ACCCGCT3300.038.18181634
AGGGCGA29350.038.177176