##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545407_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2681201
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30577789580117	31.0	31.0	33.0	30.0	34.0
2	31.656473349070062	31.0	31.0	34.0	30.0	34.0
3	31.760390213191776	31.0	31.0	34.0	30.0	34.0
4	35.46162820318208	37.0	35.0	37.0	33.0	37.0
5	35.31367845976486	37.0	35.0	37.0	33.0	37.0
6	35.38819693115138	37.0	35.0	37.0	33.0	37.0
7	35.83720243279038	37.0	35.0	37.0	35.0	37.0
8	35.86288942902826	37.0	35.0	37.0	35.0	37.0
9	37.521168312260066	39.0	37.0	39.0	35.0	39.0
10	36.90426454413526	39.0	37.0	39.0	32.0	39.0
11	36.54936873438433	39.0	35.0	39.0	32.0	39.0
12	35.70592469568675	37.0	35.0	39.0	31.0	39.0
13	35.30157753931913	37.0	35.0	39.0	30.0	39.0
14	36.27633586590487	38.0	35.0	40.0	31.0	41.0
15	36.535130711945875	38.0	35.0	40.0	31.0	41.0
16	36.70898899411122	38.0	35.0	40.0	32.0	41.0
17	36.67217974333144	38.0	35.0	40.0	32.0	41.0
18	36.61780784059084	38.0	35.0	40.0	31.0	41.0
19	36.561401774801666	38.0	35.0	40.0	31.0	41.0
20	36.41005355435866	38.0	35.0	40.0	31.0	41.0
21	36.19553177848285	37.0	35.0	40.0	31.0	41.0
22	36.11800420781582	37.0	35.0	40.0	30.0	41.0
23	36.10039829166109	37.0	34.0	40.0	30.0	41.0
24	36.018694980346496	37.0	34.0	40.0	30.0	41.0
25	35.892130429609715	37.0	34.0	40.0	30.0	41.0
26	35.80726361059838	37.0	34.0	40.0	30.0	41.0
27	35.74682949916847	37.0	34.0	40.0	30.0	41.0
28	35.73732331145632	37.0	34.0	40.0	30.0	41.0
29	35.74859960144726	36.0	34.0	40.0	30.0	41.0
30	35.68445483945441	36.0	34.0	40.0	30.0	41.0
31	35.52353590797557	36.0	34.0	40.0	29.0	41.0
32	35.342063500647654	36.0	34.0	40.0	29.0	41.0
33	35.15847226671928	36.0	34.0	40.0	28.0	41.0
34	35.01214828727872	36.0	34.0	40.0	27.0	41.0
35	34.87897401201924	36.0	34.0	40.0	27.0	41.0
36	34.69359514635419	36.0	33.0	40.0	25.0	41.0
37	34.612024984326055	36.0	33.0	40.0	25.0	41.0
38	34.61034775087731	36.0	33.0	40.0	26.0	41.0
39	34.561130627655295	36.0	33.0	40.0	25.0	41.0
40	34.3697275959542	36.0	33.0	40.0	24.0	41.0
41	34.361655467083594	35.0	33.0	40.0	24.0	41.0
42	34.27774381704318	35.0	33.0	40.0	24.0	41.0
43	34.19375384389309	35.0	33.0	40.0	24.0	41.0
44	34.133053060923075	35.0	33.0	40.0	24.0	41.0
45	34.094165636966416	35.0	33.0	40.0	24.0	41.0
46	34.07603458300963	35.0	33.0	40.0	24.0	41.0
47	34.017745778850596	35.0	33.0	40.0	24.0	41.0
48	34.000027599571986	35.0	33.0	40.0	24.0	41.0
49	33.947618623146866	35.0	33.0	39.0	24.0	41.0
50	33.79976995383785	35.0	33.0	39.0	24.0	41.0
51	33.63447574426535	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	18.0
14	35.0
15	79.0
16	231.0
17	661.0
18	1529.0
19	3090.0
20	5201.0
21	8194.0
22	11865.0
23	17129.0
24	24023.0
25	33556.0
26	43884.0
27	51666.0
28	58070.0
29	66205.0
30	78960.0
31	95674.0
32	119471.0
33	153351.0
34	257165.0
35	403954.0
36	189988.0
37	227401.0
38	313909.0
39	515558.0
40	324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41853184449804	23.784043046381083	29.668756650471188	14.128668458649688
2	30.103487205920032	26.441098597233104	29.97544756995093	13.479966626895932
3	28.564065133498012	24.82458420685357	32.7575590192604	13.85379164038802
4	26.65477149978685	27.55235433673193	30.802129344275198	14.99074481920602
5	23.489734637574728	32.619039005281586	29.40279374802561	14.48843260911808
6	21.521325704413805	41.21917752529557	26.805263760531194	10.454233009759433
7	85.88214758983008	5.388480759182173	6.752086098729635	1.9772855522581112
8	87.37658981926383	4.360806966728716	6.427418160742144	1.8351850532653091
9	82.40881604922569	5.88732437441281	8.62583595933315	3.078023617028339
10	51.60627644104265	21.816305454160283	14.863376524177038	11.714041580620028
11	45.32174201039012	21.078203387213417	20.06753689857642	13.53251770382004
12	40.186021115164436	21.39861949924679	24.437556154872386	13.977803230716384
13	21.730895967889015	40.12347451757626	23.868147147490994	14.277482367043723
14	16.020656414793223	40.93710244028702	27.291053524148317	15.751187620771438
15	15.475042714067316	23.023413761221185	46.79899045241293	14.70255307229857
16	16.616546092590596	18.69285443351692	44.94015927936771	19.750440194524767
17	17.178346569317256	19.81727591478595	28.483541517402088	34.520835998494704
18	20.982835676996988	23.578015971200966	34.550337703141246	20.888810648660805
19	29.802912948339195	23.60990466585683	26.200683947231113	20.386498438572865
20	31.5609310902092	21.90089441261584	25.788592500151985	20.749581997022975
21	22.410330296012866	28.133884777754446	28.475224349088336	20.980560577144345
22	22.57876227854607	25.723136758489947	25.074733300487356	26.62336766247663
23	20.41723093494296	29.358074982069603	25.1296340707019	25.095060012285543
24	20.105728738725666	22.920922377695668	39.21764910575522	17.755699777823445
25	17.757788394081608	24.985817922639892	36.02989854173559	21.226495141542916
26	17.195130092820342	34.153314130496	27.57838744652117	21.07316833016249
27	18.54064652370337	34.094497204797406	28.18367589748027	19.181180374018954
28	16.7138905289085	28.73581652401293	36.967649944931395	17.582643002147172
29	16.858900171975172	23.74823819624116	36.30723694344438	23.085624688339294
30	19.035238312979892	30.834950456903453	30.775723267297007	19.354087962819648
31	28.022852445601803	27.919391347384998	24.931029042582036	19.126727164431166
32	29.796572506126918	26.292060908525695	26.349162185155084	17.562204400192304
33	28.261290369502323	27.744544329201727	24.904548372166055	19.089616929129892
34	20.205609351928484	27.17386723337788	27.62746992858797	24.99305348610567
35	21.042062866603437	25.47205524688377	30.90525477202194	22.580627114490856
36	29.872396735641978	26.028335809213853	24.90995639640594	19.18931105873823
37	20.70654158341728	33.684233296944164	27.76099218223475	17.8482329374038
38	20.400223631126497	33.24547469585458	25.203444277396585	21.150857395622335
39	21.23264910016071	33.2716569925194	26.407195879756873	19.088498027563023
40	25.08726499803633	26.21108973180302	26.04217289192418	22.65947237823647
41	17.686738144585206	25.555786380804722	27.364751840686317	29.39272363392375
42	21.621579284805577	25.27113782219237	24.920697851447915	28.186585041554142
43	21.59562076845414	25.716647129402087	26.690800130240145	25.996931971903635
44	19.603080858167665	29.799929210827536	28.831967465326176	21.765022465678626
45	17.770059014598306	36.66021309107374	23.901303930589314	21.668423963738636
46	20.569774515226573	33.79168514408282	25.620943748715597	20.017596591975014
47	21.733171067741658	27.30623328873889	27.219294636992903	23.74130100652655
48	22.61106123710979	24.42174234606059	31.37646151855083	21.590734898278793
49	21.282775890356596	25.462320803251977	32.18565113171299	21.06925217467844
50	19.692182719609608	34.210378110406495	26.20154177176571	19.89589739821819
51	19.056609332907158	33.58703804750185	25.017557430420172	22.338795189170824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1399.0
1	2836.5
2	4274.0
3	11103.5
4	17933.0
5	13426.0
6	8919.0
7	9359.0
8	9799.0
9	10630.5
10	11462.0
11	11765.0
12	12068.0
13	12196.0
14	12324.0
15	11767.0
16	11210.0
17	10531.5
18	9853.0
19	10085.0
20	10317.0
21	11103.5
22	11890.0
23	11577.0
24	11264.0
25	14213.0
26	17784.0
27	18406.0
28	24831.5
29	31257.0
30	39042.0
31	46827.0
32	53194.5
33	59562.0
34	70480.0
35	81398.0
36	83589.5
37	85781.0
38	100815.0
39	115849.0
40	153218.5
41	190588.0
42	224240.0
43	257892.0
44	261534.5
45	265177.0
46	256695.0
47	248213.0
48	232233.0
49	216253.0
50	198685.5
51	181118.0
52	169141.0
53	157164.0
54	139632.0
55	122100.0
56	112731.0
57	103362.0
58	94553.0
59	85744.0
60	78878.5
61	72013.0
62	64312.5
63	56612.0
64	50238.0
65	43864.0
66	37867.5
67	31871.0
68	27707.5
69	23544.0
70	18814.5
71	14085.0
72	12005.0
73	9925.0
74	7981.0
75	4773.0
76	3509.0
77	2586.5
78	1664.0
79	1202.0
80	740.0
81	609.0
82	478.0
83	301.0
84	124.0
85	80.5
86	37.0
87	34.0
88	31.0
89	48.5
90	66.0
91	42.5
92	19.0
93	17.0
94	15.0
95	8.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2681201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.419653808791578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12437267018566	12.354900882208641
2	7.603048733384295	2.3447275872031423
3	2.682694957883137	1.24098682575446
4	1.3779760266487968	0.849916531509544
5	0.8237827865612606	0.635122269120814
6	0.5530975081961164	0.5117143259333626
7	0.3851419644742058	0.41571290316011106
8	0.28621637454014803	0.35306859278532077
9	0.23136172303471822	0.3210765906420788
>10	2.8235589291475898	10.567086578351693
>50	1.2323984533014394	13.882579129028327
>100	1.857337753922457	53.62754857758457
>500	0.017062157825894885	1.6673303504422348
>1k	0.0014624706707909905	0.527801273866379
>5k	2.437451117984984E-4	0.31852992921865425
>10k+	2.437451117984984E-4	0.3818976531906485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10161	0.3789719606997014	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8475	0.31608969264146924	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3977	0.1483290510483921	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2865	0.1068550996363197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02424286728223658	0.0
2	0.0	0.0	0.0	0.08104577016046167	0.0
3	3.7296718895748584E-5	0.0	0.0	0.1174473678027123	0.0
4	3.7296718895748584E-5	0.0	0.0	0.1935326743500394	0.0
5	7.459343779149717E-5	0.0	0.0	0.3241830806418467	0.0
6	7.459343779149717E-5	0.0	0.0	0.4965312186591009	0.0
7	7.459343779149717E-5	0.0	0.0	0.6016706692262161	0.0
8	7.459343779149717E-5	0.0	0.0	0.8804636429719368	0.0
9	7.459343779149717E-5	0.0	0.0	1.005482244710486	0.0
10	7.459343779149717E-5	0.0	0.0	1.1988284354660468	0.0
11	7.459343779149717E-5	0.0	0.0	1.3666263737780195	0.0
12	7.459343779149717E-5	0.0	0.0	1.523533670172434	0.0
13	1.1189015668724575E-4	0.0	0.0	1.5844765088480872	0.0
14	1.1189015668724575E-4	0.0	0.0	1.6062204959643085	0.0
15	1.1189015668724575E-4	0.0	0.0	1.6371021792099885	0.0
16	1.1189015668724575E-4	0.0	0.0	1.7136723431029601	0.0
17	1.1189015668724575E-4	0.0	0.0	1.8066530633100615	0.0
18	1.1189015668724575E-4	0.0	0.0	1.938459667887637	0.0
19	1.1189015668724575E-4	0.0	0.0	1.9996262868766645	0.0
20	1.1189015668724575E-4	0.0	0.0	2.0637393466584566	0.0
21	1.1189015668724575E-4	0.0	0.0	2.1585103093725535	0.0
22	1.1189015668724575E-4	0.0	0.0	2.256824460381747	0.0
23	1.1189015668724575E-4	0.0	0.0	2.3691994744146374	0.0
24	1.1189015668724575E-4	0.0	0.0	2.4569959506952297	0.0
25	1.1189015668724575E-4	0.0	0.0	2.528083496910526	0.0
26	1.1189015668724575E-4	0.0	0.0	2.6037212428311043	0.0
27	1.1189015668724575E-4	0.0	0.0	2.6736152940417375	0.0
28	1.1189015668724575E-4	0.0	0.0	2.748320621989922	0.0
29	1.1189015668724575E-4	0.0	0.0	2.8296274691826535	0.0
30	1.1189015668724575E-4	0.0	0.0	2.93633338194339	0.0
31	1.1189015668724575E-4	0.0	0.0	3.036512368897371	0.0
32	1.1189015668724575E-4	0.0	0.0	3.134901113344356	0.0
33	1.1189015668724575E-4	0.0	0.0	3.2257932172932953	0.0
34	1.1189015668724575E-4	0.0	0.0	3.3239581814269052	0.0
35	1.1189015668724575E-4	0.0	0.0	3.4536015763085275	0.0
36	1.1189015668724575E-4	0.0	0.0	3.560493972663743	0.0
37	1.1189015668724575E-4	0.0	0.0	3.671116040908533	0.0
38	1.1189015668724575E-4	0.0	0.0	3.78229755993676	0.0
39	1.1189015668724575E-4	0.0	0.0	3.896835783665604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.0349703E-4	45.000004	7
CACTACG	20	7.0349703E-4	45.000004	1
CGTACGT	25	3.8922008E-5	45.000004	39
TATCGTG	80	0.0	45.000004	1
GGTCGAA	55	1.8189894E-12	44.999996	9
TATCGAT	60	3.6379788E-12	41.249996	31
TGCGTAT	55	6.184564E-11	40.90909	36
GCGCGAC	365	0.0	40.684933	9
GTGCAAC	45	1.9299478E-8	40.0	45
CGTTTTT	8510	0.0	39.8443	1
CGTATTA	40	3.4605182E-7	39.375004	30
TAGCCCG	40	3.4605182E-7	39.375004	29
TGTTGCG	200	0.0	39.375004	1
TAATGCG	80	0.0	39.375004	1
CTTAACG	120	0.0	39.374996	1
ATTATAC	2480	0.0	38.649193	42
GGCCTAT	950	0.0	38.605263	8
ATACGTA	35	6.2514064E-6	38.571426	43
ACCCGCT	330	0.0	38.181816	34
AGGGCGA	2935	0.0	38.17717	6
>>END_MODULE