FastQCFastQC Report
Thu 26 May 2016
SRR1545406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545406_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3917462
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC137380.3506862351185538No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131390.3353957230472178No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT47170.12040959172035363No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.035369E-445.00000434
CCCGCTA502.1827873E-1145.031
CGTTTTT125050.043.0387841
TTGTACG1100.042.9545441
TCGATAG2900.042.6724131
TAACGCG1750.042.428571
GCGATCG700.041.7857139
AGAATCG1250.041.39999839
GAACGTA603.6379788E-1241.25000439
TCGAACA603.6379788E-1241.25000439
GCGTATT556.184564E-1140.90909219
CTACGAA6400.040.78125411
TATATCG501.0822987E-940.516
CCGTACA501.0822987E-940.531
TAGGGCG19650.039.7328225
GTACGAC403.461064E-739.37500445
GCTACGA6600.039.20454410
AGGGCGA40850.039.1064876
CTATACG750.039.01
TGGGCGA19700.038.832496
Produced by FastQC (version 0.11.5)