##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545406_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3917462
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296625212956755	31.0	31.0	33.0	30.0	34.0
2	31.636970569210373	31.0	31.0	34.0	30.0	34.0
3	31.724960701597105	31.0	31.0	34.0	30.0	34.0
4	35.43026301212367	37.0	35.0	37.0	33.0	37.0
5	35.282162532782706	37.0	35.0	37.0	33.0	37.0
6	35.34582160592751	37.0	35.0	37.0	33.0	37.0
7	35.85487772440422	37.0	35.0	37.0	35.0	37.0
8	35.89607888985267	37.0	35.0	37.0	35.0	37.0
9	37.57130815819018	39.0	37.0	39.0	35.0	39.0
10	36.87885932269413	39.0	37.0	39.0	32.0	39.0
11	36.51575331171049	39.0	35.0	39.0	32.0	39.0
12	35.599115447705685	37.0	35.0	39.0	31.0	39.0
13	35.179332945667376	37.0	35.0	39.0	30.0	39.0
14	36.11357353306809	38.0	35.0	40.0	30.0	41.0
15	36.408999244919286	38.0	35.0	40.0	31.0	41.0
16	36.59947869309262	38.0	35.0	40.0	32.0	41.0
17	36.56499999234198	38.0	35.0	40.0	31.0	41.0
18	36.489757909585336	38.0	35.0	40.0	31.0	41.0
19	36.44566175753587	37.0	35.0	40.0	31.0	41.0
20	36.281219575327086	37.0	35.0	40.0	31.0	41.0
21	36.07325840046438	37.0	34.0	40.0	30.0	41.0
22	35.99633767985497	37.0	34.0	40.0	30.0	41.0
23	35.967383474300455	37.0	34.0	40.0	30.0	41.0
24	35.89934401405808	36.0	34.0	40.0	30.0	41.0
25	35.82630846195828	36.0	34.0	40.0	30.0	41.0
26	35.71481050741526	36.0	34.0	40.0	30.0	41.0
27	35.67016399903815	36.0	34.0	40.0	30.0	41.0
28	35.68587187316686	36.0	34.0	40.0	30.0	41.0
29	35.72301377779797	36.0	34.0	40.0	30.0	41.0
30	35.649982565242496	36.0	34.0	40.0	30.0	41.0
31	35.46738041109269	36.0	34.0	40.0	29.0	41.0
32	35.2625564715114	36.0	34.0	40.0	29.0	41.0
33	35.093625924131494	36.0	34.0	40.0	28.0	41.0
34	34.930690584873574	36.0	34.0	40.0	27.0	41.0
35	34.77299333088617	36.0	34.0	40.0	27.0	41.0
36	34.60016000155203	36.0	33.0	40.0	25.0	41.0
37	34.51532905743566	36.0	33.0	40.0	25.0	41.0
38	34.51842417361036	35.0	33.0	40.0	25.0	41.0
39	34.47490900996615	36.0	33.0	40.0	25.0	41.0
40	34.28671088577247	36.0	33.0	40.0	24.0	41.0
41	34.314256271024455	35.0	33.0	40.0	24.0	41.0
42	34.2336464272021	35.0	33.0	40.0	24.0	41.0
43	34.15105136948361	35.0	33.0	40.0	24.0	41.0
44	34.08940456857016	35.0	33.0	40.0	23.0	41.0
45	34.045730628657026	35.0	33.0	40.0	23.0	41.0
46	34.023980066686036	35.0	33.0	40.0	23.0	41.0
47	33.98953480595345	35.0	33.0	40.0	23.0	41.0
48	33.97837987962615	35.0	33.0	40.0	23.0	41.0
49	33.94980729870513	35.0	33.0	39.0	24.0	41.0
50	33.804119861277535	35.0	33.0	39.0	24.0	41.0
51	33.61882412643696	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	5.0
13	9.0
14	25.0
15	126.0
16	347.0
17	992.0
18	2272.0
19	4536.0
20	7561.0
21	12125.0
22	17944.0
23	26087.0
24	37066.0
25	51620.0
26	67541.0
27	78561.0
28	86470.0
29	97703.0
30	115476.0
31	140882.0
32	174876.0
33	225241.0
34	381764.0
35	608257.0
36	263987.0
37	317510.0
38	448649.0
39	749469.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70306131878242	23.712163640642846	29.287457032129478	14.297318008445265
2	30.46477540815967	25.399301894951375	31.156013766055672	12.979908930833279
3	28.538068780245986	25.392690471534884	31.91586797778766	14.153372770431469
4	25.03383568238824	28.496255994314684	31.64508041175639	14.824827911540686
5	22.53982297722352	32.28945169091621	30.527622220713308	14.643103111146964
6	21.087862498730043	39.96809669117403	28.8346893983911	10.109351411704823
7	86.67542914264389	4.511594496640938	6.993737271733587	1.8192390889815908
8	87.9182235845555	3.5333846250455014	6.5124818058222385	2.0359099845767488
9	82.96246396263703	5.353389515967226	8.577492264124068	3.106654257271672
10	52.06799708586834	19.767390213357526	16.664743652905887	11.499869047868238
11	46.93679223946525	21.492001709269932	18.764291778707744	12.80691427255708
12	40.18959213899203	21.76281480203254	24.267625314553147	13.779967744422281
13	20.926431449749863	40.86793949756245	24.284243216654048	13.921385836033636
14	16.278243413720414	42.715079303896246	27.08312167418599	13.923555608197349
15	15.141180693009911	23.362677161897167	47.96342632040847	13.532715824684452
16	15.810389481761405	19.035768566485135	46.11235539744866	19.0414865543048
17	16.817750880544597	19.191813475153047	29.032113138557563	34.958322505744796
18	20.446707587718784	23.76523371509411	35.422551641853836	20.365507055333275
19	29.247737438167874	24.339942544433104	26.468565617228705	19.943754400170313
20	31.432238525861898	22.153884326127475	26.30527111686087	20.10860603114976
21	21.983212600403014	28.586467462862437	28.748945107827467	20.681374828907085
22	22.85734998833428	24.951078019391126	25.32062850896831	26.87094348330628
23	19.57956452417407	30.81622744521836	24.786073228023653	24.81813480258392
24	20.000576904128234	22.908275817353175	39.98790543469216	17.103241843826435
25	16.95398704569438	25.53308749389273	36.38664523101947	21.12628022939342
26	16.681948669827555	34.39048036713566	28.74452898330603	20.183041979730753
27	17.83343399374391	35.04447522400983	28.38363205565236	18.7384587265939
28	15.394635608462826	29.692259937684145	37.29590740127154	17.617197052581492
29	15.370334160229252	25.11202406047589	37.33542278138244	22.182218997912422
30	18.607455541368363	31.187284011944467	31.24964581660269	18.955614630084476
31	27.789829231272694	27.35648744008238	26.206431613120944	18.64725171552398
32	29.02642067746924	26.224785332952816	27.72432763865993	17.02446635091802
33	28.482956567287697	28.00900174653896	24.93828912699089	18.56975255918245
34	19.89619809968801	27.596847142359003	28.023398823013473	24.48355593493951
35	20.06115183759281	26.51081235759275	30.853215678926816	22.57482012588763
36	29.893819008327334	24.56610938408592	26.77749011987863	18.762581487708115
37	20.72369814946514	33.13864946233046	28.621209344213167	17.516443043991238
38	20.819831819683255	32.67531376181824	25.073070268454423	21.431784150044084
39	20.590040184180474	31.94517266536344	27.329122784088266	20.135664366367816
40	23.781672930075644	25.894775750217867	26.827828834076755	23.495722485629727
41	17.534592549972405	24.968921204596242	27.437585865542534	30.058900379888815
42	21.390839272978273	25.388453034132812	25.806172465744403	27.414535227144516
43	21.582417391668383	26.03810834667956	26.854836115832136	25.52463814581992
44	19.200007555912478	30.249049001624012	29.418536797549027	21.13240664491449
45	17.392766030659644	37.65496640426888	23.199152920947284	21.75311464412418
46	21.257028147305576	33.50924654789249	26.02909741051732	19.204627894284616
47	21.778998749700698	27.826536670936434	26.738919228827235	23.65554535053563
48	22.178874996107172	25.324814892907703	31.242957812992188	21.253352297992937
49	21.566233444000222	24.75587510485105	32.229336238615716	21.448555212533012
50	19.287896091908486	33.89873341464448	25.967424827605218	20.845945665841814
51	18.66249117413264	33.667716496037485	24.846316313980836	22.823476015849035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2050.0
1	3286.5
2	4523.0
3	14092.5
4	23662.0
5	18105.0
6	12548.0
7	13532.5
8	14517.0
9	16219.5
10	17922.0
11	18720.5
12	19519.0
13	19315.0
14	19111.0
15	18607.0
16	18103.0
17	16788.0
18	15473.0
19	16284.0
20	17095.0
21	15506.5
22	13918.0
23	14842.5
24	15767.0
25	18204.0
26	24229.0
27	27817.0
28	35019.0
29	42221.0
30	49250.0
31	56279.0
32	68106.5
33	79934.0
34	98600.5
35	117267.0
36	118329.5
37	119392.0
38	145728.5
39	172065.0
40	236223.0
41	300381.0
42	364037.0
43	427693.0
44	430708.0
45	433723.0
46	411368.0
47	389013.0
48	355459.5
49	321906.0
50	297337.0
51	272768.0
52	245702.5
53	218637.0
54	194950.0
55	171263.0
56	155663.5
57	140064.0
58	123637.0
59	107210.0
60	99446.5
61	91683.0
62	80404.5
63	69126.0
64	59525.5
65	49925.0
66	42108.5
67	34292.0
68	28029.0
69	21766.0
70	18115.0
71	14464.0
72	11766.0
73	9068.0
74	8624.5
75	5826.0
76	3471.0
77	2648.0
78	1825.0
79	1177.0
80	529.0
81	483.0
82	437.0
83	264.5
84	92.0
85	79.0
86	66.0
87	43.0
88	20.0
89	17.0
90	14.0
91	8.5
92	3.0
93	6.0
94	9.0
95	8.5
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3917462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.207040428024815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77915392252378	12.132048190134334
2	8.330851258635914	2.533751837798755
3	2.959128386870329	1.3499875503255883
4	1.5122266954036967	0.9198596997336952
5	0.93899927246323	0.7139699949117114
6	0.6384977326758854	0.582579650040262
7	0.44915845458248455	0.4781259544197493
8	0.35183178357469497	0.42802561253475707
9	0.2652977206215496	0.3630953846659264
>10	2.245086553055718	7.589726412316085
>50	0.7999359950614675	8.868675723631204
>100	1.6546369420032188	56.16557553928266
>500	0.0719774663837512	6.5583713887700315
>1k	0.002879098655350048	0.6240046352975027
>5k	0.0	0.0
>10k+	3.387174888647115E-4	0.6922024261377268
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	13738	0.3506862351185538	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13139	0.3353957230472178	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4717	0.12040959172035363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.552673133778962E-5	0.0	0.0	0.03624795849966126	0.0
2	2.552673133778962E-5	0.0	0.0	0.11949062939219321	0.0
3	2.552673133778962E-5	0.0	0.0	0.17227990979874214	0.0
4	2.552673133778962E-5	0.0	0.0	0.28689493350541756	0.0
5	2.552673133778962E-5	0.0	0.0	0.4836294519257621	0.0
6	2.552673133778962E-5	0.0	0.0	0.7254186511573054	0.0
7	5.105346267557924E-5	0.0	0.0	0.8794724747808658	0.0
8	5.105346267557924E-5	0.0	0.0	1.2579828470576102	0.0
9	5.105346267557924E-5	0.0	0.0	1.4073397521150173	0.0
10	5.105346267557924E-5	0.0	0.0	1.6552808935989678	0.0
11	7.658019401336886E-5	0.0	0.0	1.8759339592828215	0.0
12	7.658019401336886E-5	0.0	0.0	2.081424146552028	0.0
13	1.0210692535115848E-4	0.0	0.0	2.1603527998484733	0.0
14	1.0210692535115848E-4	0.0	0.0	2.1895809072302423	0.0
15	1.0210692535115848E-4	0.0	0.0	2.2308321050721105	0.0
16	1.0210692535115848E-4	0.0	0.0	2.3306671513342057	0.0
17	1.0210692535115848E-4	0.0	0.0	2.449162238204225	0.0
18	1.0210692535115848E-4	0.0	0.0	2.6265474942705254	0.0
19	1.276336566889481E-4	0.0	0.0	2.7057314148803484	0.0
20	1.5316038802673773E-4	0.0	0.0	2.7900717352204056	0.0
21	1.7868711936452734E-4	0.0	0.0	2.9107876477168126	0.0
22	1.7868711936452734E-4	0.0	0.0	3.0345922947050923	0.0
23	1.7868711936452734E-4	0.0	0.0	3.190892470686378	0.0
24	1.7868711936452734E-4	0.0	0.0	3.3066051438405784	0.0
25	1.7868711936452734E-4	0.0	0.0	3.4015645844171556	0.0
26	1.7868711936452734E-4	0.0	0.0	3.4984130031127294	0.0
27	1.7868711936452734E-4	0.0	0.0	3.5919684734657285	0.0
28	1.7868711936452734E-4	0.0	0.0	3.6873363417437104	0.0
29	1.7868711936452734E-4	0.0	0.0	3.7953399420338987	0.0
30	1.7868711936452734E-4	0.0	0.0	3.9371409346153197	0.0
31	1.7868711936452734E-4	0.0	0.0	4.07051810585527	0.0
32	1.7868711936452734E-4	0.0	0.0	4.191259545083016	0.0
33	1.7868711936452734E-4	0.0	0.0	4.313634695116379	0.0
34	1.7868711936452734E-4	0.0	0.0	4.441013084491949	0.0
35	2.0421385070231696E-4	0.0	0.0	4.604690485829856	0.0
36	2.2974058204010657E-4	0.0	0.0	4.740186375770843	0.0
37	2.2974058204010657E-4	0.0	0.0	4.88088971890474	0.0
38	2.552673133778962E-4	0.0	0.0	5.013475561473219	0.0
39	2.807940447156858E-4	0.0	0.0	5.150528582025812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.035369E-4	45.000004	34
CCCGCTA	50	2.1827873E-11	45.0	31
CGTTTTT	12505	0.0	43.038784	1
TTGTACG	110	0.0	42.954544	1
TCGATAG	290	0.0	42.672413	1
TAACGCG	175	0.0	42.42857	1
GCGATCG	70	0.0	41.785713	9
AGAATCG	125	0.0	41.399998	39
GAACGTA	60	3.6379788E-12	41.250004	39
TCGAACA	60	3.6379788E-12	41.250004	39
GCGTATT	55	6.184564E-11	40.909092	19
CTACGAA	640	0.0	40.781254	11
TATATCG	50	1.0822987E-9	40.5	16
CCGTACA	50	1.0822987E-9	40.5	31
TAGGGCG	1965	0.0	39.732822	5
GTACGAC	40	3.461064E-7	39.375004	45
GCTACGA	660	0.0	39.204544	10
AGGGCGA	4085	0.0	39.106487	6
CTATACG	75	0.0	39.0	1
TGGGCGA	1970	0.0	38.83249	6
>>END_MODULE