FastQCFastQC Report
Thu 26 May 2016
SRR1545405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545405_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5044713
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194900.3863450705719037No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC160240.3176394772110921No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT122990.24379979594478418No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG253.892692E-545.00000428
GTCGACG351.2130295E-745.014
CGCGATA351.2130295E-745.011
ATCGCTA207.035563E-445.013
ATCGTAC302.1669712E-644.99999611
GCGATAC1600.043.593759
TTACGCG1200.043.1249961
AGTACGG3050.042.0491832
CGTAAGG6500.041.8846172
TACGGGA17300.041.7485544
CGTTTTT164650.041.7203141
GCTACGA14750.041.6440710
ACGGGAT16550.041.0574045
CGAATAT15100.040.9768214
CTCGACT556.184564E-1140.90909213
TCGACTA556.184564E-1140.90909214
GTCGATG3100.040.6451641
GACGATT1000.040.50000419
CTACGGG13050.040.3448263
TATTACG3350.040.2985081
Produced by FastQC (version 0.11.5)