##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545405_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5044713
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25013038402779	31.0	31.0	33.0	30.0	34.0
2	31.59028610745547	31.0	31.0	34.0	30.0	34.0
3	31.686866428278478	31.0	31.0	34.0	30.0	34.0
4	35.387998881204936	37.0	35.0	37.0	33.0	37.0
5	35.23764602664215	37.0	35.0	37.0	32.0	37.0
6	35.31026641158773	37.0	35.0	37.0	32.0	37.0
7	35.806335662702715	37.0	35.0	37.0	35.0	37.0
8	35.8458378107932	37.0	35.0	37.0	35.0	37.0
9	37.52716854259103	39.0	37.0	39.0	35.0	39.0
10	36.87087808563143	39.0	37.0	39.0	32.0	39.0
11	36.4936366052935	39.0	35.0	39.0	32.0	39.0
12	35.644750652812164	37.0	35.0	39.0	31.0	39.0
13	35.23712389584898	37.0	35.0	39.0	30.0	39.0
14	36.17835781738228	38.0	35.0	40.0	30.0	41.0
15	36.443203409192954	38.0	35.0	40.0	31.0	41.0
16	36.64306096303199	38.0	35.0	40.0	32.0	41.0
17	36.58908484982198	38.0	35.0	40.0	31.0	41.0
18	36.52185109440319	38.0	35.0	40.0	31.0	41.0
19	36.48256223892221	37.0	35.0	40.0	31.0	41.0
20	36.310825412664705	37.0	35.0	40.0	31.0	41.0
21	36.099730549587264	37.0	34.0	40.0	30.0	41.0
22	36.03478215708208	37.0	34.0	40.0	30.0	41.0
23	36.00104327044968	37.0	34.0	40.0	30.0	41.0
24	35.92778102540224	37.0	34.0	40.0	30.0	41.0
25	35.82567432478319	37.0	34.0	40.0	30.0	41.0
26	35.73442770678927	37.0	34.0	40.0	30.0	41.0
27	35.695215763513204	37.0	34.0	40.0	30.0	41.0
28	35.69075842372004	36.0	34.0	40.0	30.0	41.0
29	35.70555054370784	36.0	34.0	40.0	30.0	41.0
30	35.61100304417714	36.0	34.0	40.0	30.0	41.0
31	35.438068528378125	36.0	34.0	40.0	29.0	41.0
32	35.25882978873129	36.0	34.0	40.0	29.0	41.0
33	35.07171845058381	36.0	34.0	40.0	27.0	41.0
34	34.9033748797999	36.0	34.0	40.0	27.0	41.0
35	34.76270721446394	36.0	34.0	40.0	26.0	41.0
36	34.56424220763401	36.0	33.0	40.0	25.0	41.0
37	34.49279671608672	36.0	33.0	40.0	25.0	41.0
38	34.500503596537605	36.0	33.0	40.0	25.0	41.0
39	34.456057064098594	36.0	33.0	40.0	25.0	41.0
40	34.28432360770573	36.0	33.0	40.0	24.0	41.0
41	34.26057339634584	36.0	33.0	40.0	24.0	41.0
42	34.174312988667545	35.0	33.0	40.0	24.0	41.0
43	34.09357678028463	35.0	33.0	40.0	23.0	41.0
44	34.026132705666306	35.0	33.0	40.0	23.0	41.0
45	33.9784275141123	35.0	33.0	40.0	23.0	41.0
46	33.9548253785696	35.0	33.0	40.0	23.0	41.0
47	33.89733489298598	35.0	33.0	40.0	23.0	41.0
48	33.90711562778695	35.0	33.0	39.0	23.0	41.0
49	33.87540678726421	35.0	33.0	39.0	24.0	41.0
50	33.72344234449016	35.0	33.0	39.0	24.0	41.0
51	33.55664137880589	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	5.0
13	11.0
14	42.0
15	143.0
16	497.0
17	1359.0
18	3083.0
19	6029.0
20	10055.0
21	15877.0
22	23498.0
23	33915.0
24	47448.0
25	66031.0
26	86253.0
27	102394.0
28	113619.0
29	129782.0
30	152996.0
31	184824.0
32	229425.0
33	292284.0
34	482714.0
35	749678.0
36	353105.0
37	422581.0
38	581931.0
39	954674.0
40	457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.17552713107762	24.22518387071772	29.823658947496124	13.775630050708534
2	30.210638345531255	25.781387365346653	30.166057018506304	13.841917270615792
3	27.952610981040944	24.8899788749132	32.621697210525156	14.535712933520697
4	25.213029958294953	27.67701552100189	32.03857186722019	15.071382653482962
5	22.479990437513493	32.3646558287855	30.705354298648903	14.449999435052103
6	20.372159922675483	39.86809556856852	28.76338059271162	10.99636391604438
7	85.41693055680274	5.000522329020502	7.466192824051636	2.1163542901251273
8	86.79254895174412	3.8389696301851064	6.983211929003691	2.3852694890670687
9	82.58342942403264	5.287258165132487	8.96802256144205	3.1612898493928197
10	50.83718340369412	23.17124879056549	14.99752314948343	10.994044656256957
11	43.2628972153619	21.022048231485122	21.452379947085195	14.26267460606778
12	38.927427586068816	21.38900666896214	26.228647695121605	13.454918049847434
13	21.105283888300484	41.16547363546747	24.050466300065036	13.678776176167009
14	15.341249343619745	41.73771629823143	27.473317114373007	15.447717243775811
15	13.916093938346938	23.14714434696285	48.57152428691186	14.365237427778348
16	15.648462063154039	18.565932293868848	45.366346906157	20.41925873682011
17	15.879614955300728	19.5402989228525	29.0715844489072	35.508501672939566
18	20.482711305876073	22.414119495003977	36.500153725296165	20.60301547382378
19	28.168064268472754	24.857826401620862	25.70576363809002	21.26834569181636
20	30.865878792311875	22.02969722955498	26.870983542572198	20.233440435560954
21	21.795055536360543	27.61863757165175	28.76338059271162	21.822926299276094
22	22.594308140026996	24.278051100231075	24.985881258259884	28.141759501482046
23	19.01154733678606	29.531134873282188	25.605619189833	25.851698600098754
24	18.927280897842948	22.634191479277412	40.82103382293502	17.617493799944615
25	16.693040813223668	26.13914805460687	36.442073116944414	20.725738015225048
26	17.17076868396676	33.55875745557775	27.283474798268998	21.98699906218649
27	16.8747756314383	34.63211881429132	28.372000547900345	20.121105006370037
28	15.369338156600781	28.872742611918657	37.24402161232958	18.513897619150978
29	15.476717902485234	23.282156982964146	36.77691872659555	24.464206387955073
30	18.03404871595272	30.970820342009546	31.996686431913968	18.998444510123765
31	26.73989580774962	28.2798843065998	25.790267949831836	19.189951935818748
32	28.495099721232904	25.80430244495574	27.09044102211563	18.61015681169573
33	27.8153385534519	27.911419341397618	24.387928510501986	19.885313594648498
34	18.838554343924024	27.03955606592486	28.406274053647852	25.715615536503268
35	19.116191545485343	26.86483849527218	30.819513419296594	23.19945653994588
36	28.928365201350402	26.040510134075024	25.870074273799126	19.161050390775454
37	19.3889523546731	33.32484920351267	29.248165356483113	18.03803308533112
38	19.07466291937718	32.328499163381544	26.938460126473	21.658377790768277
39	19.393095305917303	33.07944376617659	28.148974976376255	19.37848595152985
40	23.911726990217282	26.571719739061468	26.777440064479386	22.73911320624186
41	17.032049196852228	24.887560501459646	28.416899038656908	29.663491263031215
42	20.86687587579313	25.587778729929727	25.95941533244805	27.58593006182909
43	20.73485647250894	25.435718543354202	27.986646613989734	25.842778370147123
44	19.097102253388844	29.609870769655284	29.456105828022327	21.83692114893355
45	17.12341217429019	37.41768857812129	23.21547330839237	22.243425939196147
46	21.25167477317342	32.70796574552408	25.990774896411352	20.049584584891154
47	19.657213403418588	29.491846216028545	27.42629759116128	23.424642789391587
48	20.93244947730426	24.977317837506316	31.377265664072468	22.71296702111696
49	20.73477718157604	24.69298055211466	32.618783268740955	21.953458997568344
50	19.249994994759863	33.52987573326768	27.35993108032112	19.86019819165134
51	17.64889300937437	32.26653329931752	26.51246958944939	23.572104101858717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3038.0
1	5142.5
2	7247.0
3	20327.5
4	33408.0
5	25324.0
6	17240.0
7	18677.5
8	20115.0
9	21833.5
10	23552.0
11	24863.0
12	26174.0
13	25528.0
14	24882.0
15	24112.0
16	23342.0
17	22585.5
18	21829.0
19	22090.5
20	22352.0
21	22177.5
22	22003.0
23	22560.0
24	23117.0
25	26149.5
26	37385.5
27	45589.0
28	51185.5
29	56782.0
30	64734.5
31	72687.0
32	96829.5
33	120972.0
34	148274.0
35	175576.0
36	180583.0
37	185590.0
38	216192.0
39	246794.0
40	308256.5
41	369719.0
42	432523.0
43	495327.0
44	496781.5
45	498236.0
46	488014.5
47	477793.0
48	448169.0
49	418545.0
50	383737.5
51	348930.0
52	306594.5
53	264259.0
54	240625.0
55	216991.0
56	197048.5
57	177106.0
58	160446.0
59	143786.0
60	132104.0
61	120422.0
62	109570.0
63	98718.0
64	85987.0
65	73256.0
66	61687.5
67	50119.0
68	42486.5
69	34854.0
70	27486.5
71	20119.0
72	17752.0
73	15385.0
74	12320.0
75	7530.5
76	5806.0
77	4138.0
78	2470.0
79	1695.5
80	921.0
81	834.0
82	747.0
83	541.5
84	336.0
85	219.0
86	102.0
87	61.5
88	21.0
89	15.5
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5044713.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.683510390939944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44103123551437	11.664732076136598
2	8.789778239930449	2.581296002401531
3	3.082659120843274	1.3579277169788402
4	1.5405151359659062	0.9048068002542258
5	0.9802854269805261	0.7197015626577776
6	0.6568930387742214	0.5787297456346439
7	0.47524266731708237	0.48847614506378756
8	0.34851599174954473	0.40939505490105443
9	0.2946882473314902	0.38943521476018345
>10	2.071578484043631	6.450922481943221
>50	0.6422742321119292	6.97672742814507
>100	1.505159616425975	50.790691686398084
>500	0.16291205232889655	14.19508151082583
>1k	0.008056840810901002	1.5333634771630749
>5k	0.0	0.0
>10k+	4.0966987174072896E-4	0.9587130967360354
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19490	0.3863450705719037	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	16024	0.3176394772110921	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	12299	0.24379979594478418	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05841759481659314	0.0
2	0.0	0.0	0.0	0.19305359888659673	0.0
3	0.0	0.0	0.0	0.27284010012066096	0.0
4	0.0	0.0	0.0	0.4347125396429886	0.0
5	0.0	0.0	0.0	0.7199616707630345	0.0
6	0.0	0.0	0.0	1.057978917730305	0.0
7	0.0	0.0	0.0	1.2714895773059836	0.0
8	0.0	0.0	0.0	1.7719144775926796	0.0
9	0.0	0.0	0.0	1.9532528411427965	0.0
10	0.0	0.0	0.0	2.2266479777937813	0.0
11	0.0	0.0	0.0	2.4949288492724957	0.0
12	0.0	0.0	0.0	2.7392043908147006	0.0
13	0.0	0.0	0.0	2.8386550434088123	0.0
14	0.0	0.0	0.0	2.8762389456050323	0.0
15	0.0	0.0	0.0	2.9335662900942037	0.0
16	1.9822733225854474E-5	0.0	0.0	3.0519476529190066	0.0
17	1.9822733225854474E-5	0.0	0.0	3.1989530425219432	0.0
18	3.964546645170895E-5	0.0	0.0	3.4081423462543854	0.0
19	7.92909329034179E-5	0.0	0.0	3.5025976700755823	0.0
20	9.911366612927237E-5	0.0	0.0	3.601374349740015	0.0
21	9.911366612927237E-5	0.0	0.0	3.740807455250675	0.0
22	9.911366612927237E-5	0.0	0.0	3.8903105092400696	0.0
23	9.911366612927237E-5	0.0	0.0	4.071430822724702	0.0
24	9.911366612927237E-5	0.0	0.0	4.211359496566009	0.0
25	1.1893639935512684E-4	0.0	0.0	4.323258825625957	0.0
26	1.387591325809813E-4	0.0	0.0	4.430559280577508	0.0
27	1.585818658068358E-4	0.0	0.0	4.536947889800668	0.0
28	1.585818658068358E-4	0.0	0.0	4.64486284948222	0.0
29	1.585818658068358E-4	0.0	0.0	4.7766642027009265	0.0
30	1.7840459903269027E-4	0.0	0.0	4.954513765203293	0.0
31	1.7840459903269027E-4	0.0	0.0	5.105047601320432	0.0
32	1.7840459903269027E-4	0.0	0.0	5.2379590275997865	0.0
33	1.7840459903269027E-4	0.0	0.0	5.366212111571064	0.0
34	1.7840459903269027E-4	0.0	0.0	5.5045351440210775	0.0
35	1.7840459903269027E-4	0.0	0.0	5.698401474969934	0.0
36	1.7840459903269027E-4	0.0	0.0	5.845228459973838	0.0
37	1.7840459903269027E-4	0.0	0.0	5.994275591099038	0.0
38	1.9822733225854474E-4	0.0	0.0	6.1393185301126145	0.0
39	1.9822733225854474E-4	0.0	0.0	6.310884286182385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTCG	25	3.892692E-5	45.000004	28
GTCGACG	35	1.2130295E-7	45.0	14
CGCGATA	35	1.2130295E-7	45.0	11
ATCGCTA	20	7.035563E-4	45.0	13
ATCGTAC	30	2.1669712E-6	44.999996	11
GCGATAC	160	0.0	43.59375	9
TTACGCG	120	0.0	43.124996	1
AGTACGG	305	0.0	42.049183	2
CGTAAGG	650	0.0	41.884617	2
TACGGGA	1730	0.0	41.748554	4
CGTTTTT	16465	0.0	41.720314	1
GCTACGA	1475	0.0	41.64407	10
ACGGGAT	1655	0.0	41.057404	5
CGAATAT	1510	0.0	40.97682	14
CTCGACT	55	6.184564E-11	40.909092	13
TCGACTA	55	6.184564E-11	40.909092	14
GTCGATG	310	0.0	40.645164	1
GACGATT	100	0.0	40.500004	19
CTACGGG	1305	0.0	40.344826	3
TATTACG	335	0.0	40.298508	1
>>END_MODULE