FastQCFastQC Report
Thu 26 May 2016
SRR1545404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545404_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3924724
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154830.39449907815173757No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC121650.30995810151236114No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT64200.16357838156262708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTT302.1668475E-645.00000438
GTCGAAC406.8230293E-945.029
CCGTCTA207.0353725E-445.028
TACGATA453.8562575E-1045.044
CCGGATA1150.044.99999620
CGGTCTA3950.042.72151631
CGACGGT3750.042.60000228
ACGTTAG2450.042.24491
GAGTACG800.042.18751
ATCGTAG2200.041.931821
TATAGCG2050.041.7073171
TCACGAC3900.040.38461725
CGTTTTT131000.040.2595441
ATCGGCG451.9303116E-840.011
GTCGGTA900.040.010
GCGTAAG3150.039.9999961
GTTAGCG2550.039.7058831
GCGATCG403.461064E-739.3759
AGGGCGA34950.039.2703866
TTAGCGG9800.039.0306132
Produced by FastQC (version 0.11.5)