FastQCFastQC Report
Thu 26 May 2016
SRR1545403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545403_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1586800
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC68950.43452230904965966No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60450.3809553819006806No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20160.1270481472145198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA302.1660271E-645.00000424
CTCGGTT302.1660271E-645.00000422
AGGCACG302.1660271E-645.0000041
TGTTACG600.045.0000041
ACTATCG207.0340995E-445.033
AACCCGC207.0340995E-445.025
ATTACGA207.0340995E-445.011
CGGCACT253.89148E-545.031
CTAACGG253.89148E-545.02
TTACGTA406.8175723E-945.018
CGTAAAG406.8175723E-945.030
ACCGTTC207.0340995E-445.011
ACGTATG253.89148E-545.01
ACGTAAG351.21232E-745.01
CGGTCCA253.89148E-545.034
TTCGCAC253.89148E-545.044
CCACGGT253.89148E-545.034
ATGCACG351.21232E-745.01
CGATATC253.89148E-545.010
ATCGCAA207.0340995E-445.014