##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545403_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1586800
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305340307537183	31.0	31.0	33.0	30.0	34.0
2	31.64776090244517	31.0	31.0	34.0	30.0	34.0
3	31.74284408873204	31.0	31.0	34.0	30.0	34.0
4	35.439816612049405	37.0	35.0	37.0	33.0	37.0
5	35.28915679354676	37.0	35.0	37.0	33.0	37.0
6	35.362505041593145	37.0	35.0	37.0	33.0	37.0
7	35.8413461053693	37.0	35.0	37.0	35.0	37.0
8	35.88143055205445	37.0	35.0	37.0	35.0	37.0
9	37.559971641038565	39.0	37.0	39.0	35.0	39.0
10	36.9143408116965	39.0	37.0	39.0	32.0	39.0
11	36.52345538190068	39.0	35.0	39.0	32.0	39.0
12	35.44533967733804	37.0	35.0	39.0	31.0	39.0
13	34.96206138139652	37.0	34.0	39.0	30.0	39.0
14	35.881397781699015	37.0	35.0	40.0	30.0	41.0
15	36.20896269221074	37.0	35.0	40.0	31.0	41.0
16	36.4378831610789	37.0	35.0	40.0	32.0	41.0
17	36.40652886312075	37.0	35.0	40.0	31.0	41.0
18	36.33849004285354	37.0	35.0	40.0	31.0	41.0
19	36.279521678850514	37.0	35.0	40.0	31.0	41.0
20	36.079745399546255	36.0	35.0	40.0	31.0	41.0
21	35.86829468111923	36.0	34.0	40.0	30.0	41.0
22	35.79725233173683	36.0	34.0	40.0	30.0	41.0
23	35.78091820015125	36.0	34.0	40.0	30.0	41.0
24	35.72008885807915	35.0	34.0	40.0	30.0	41.0
25	35.58552621628434	35.0	34.0	40.0	30.0	41.0
26	35.47803440887321	35.0	34.0	40.0	30.0	41.0
27	35.40418326191077	35.0	34.0	40.0	30.0	41.0
28	35.44699395008823	36.0	34.0	40.0	30.0	41.0
29	35.48785417191833	36.0	34.0	40.0	30.0	41.0
30	35.41859528611041	36.0	34.0	40.0	30.0	41.0
31	35.203575119737835	36.0	34.0	40.0	29.0	41.0
32	34.98953302243509	35.0	34.0	40.0	29.0	41.0
33	34.799760524325684	35.0	34.0	40.0	27.0	41.0
34	34.63282140156289	35.0	34.0	40.0	27.0	41.0
35	34.4806150743635	35.0	33.0	40.0	26.0	41.0
36	34.2655035291152	35.0	33.0	40.0	24.0	41.0
37	34.19469183261911	35.0	33.0	40.0	24.0	41.0
38	34.21204499621881	35.0	33.0	40.0	24.0	41.0
39	34.15566486009579	35.0	33.0	40.0	24.0	41.0
40	33.99295185278548	35.0	33.0	40.0	23.0	41.0
41	34.002229644567684	35.0	33.0	40.0	23.0	41.0
42	33.91820834383665	35.0	33.0	40.0	23.0	41.0
43	33.81004096294429	35.0	33.0	40.0	23.0	41.0
44	33.76700340307537	35.0	33.0	39.0	23.0	41.0
45	33.72148474918074	35.0	33.0	39.0	23.0	41.0
46	33.70715843206453	35.0	33.0	39.0	23.0	41.0
47	33.67323166120494	35.0	33.0	39.0	23.0	41.0
48	33.6692292664482	35.0	33.0	39.0	23.0	41.0
49	33.65704436601966	35.0	33.0	39.0	24.0	41.0
50	33.507166624653394	35.0	33.0	39.0	24.0	41.0
51	33.32584887824552	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	16.0
15	51.0
16	161.0
17	438.0
18	945.0
19	1901.0
20	3268.0
21	5064.0
22	7748.0
23	11208.0
24	15954.0
25	22188.0
26	28604.0
27	33416.0
28	36598.0
29	41137.0
30	48727.0
31	58801.0
32	73437.0
33	94390.0
34	165701.0
35	272800.0
36	103917.0
37	119358.0
38	164613.0
39	276158.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.121754474413915	25.24476934711369	29.06503655155029	14.568439626922109
2	29.02287622888833	26.49905470128561	30.640597428787498	13.837471641038569
3	28.03195109654651	25.218805142425005	32.539009326947316	14.21023443408117
4	24.72447693471137	27.510272246029743	32.70046634736577	15.06478447189312
5	22.634106377615325	32.32593899672296	31.47302747668263	13.566927148979078
6	21.364255104613058	41.230211746912026	27.302810688177466	10.102722460297455
7	85.55715906226368	5.479203428283338	7.213448449710108	1.7501890597428786
8	87.61980085707084	3.840685656667507	6.7631081421729276	1.7764053440887322
9	83.09887824552558	5.563271993950089	8.44945802873708	2.888391731787245
10	55.175006301991424	19.112490547012857	15.086085202924124	10.626417948071591
11	50.19460549533653	19.688051424250062	17.735064280312578	12.382278800100831
12	43.10562137635493	21.722271237711116	22.865263423241743	12.306843962692211
13	20.25668011091505	44.66265439879002	22.500126039828586	12.580539450466347
14	14.39217292664482	45.293105621376355	26.9919964708848	13.322724981094025
15	13.406100327703555	22.742752709856315	50.69120241996471	13.159944542475422
16	14.236324678598436	17.66895639021931	49.22315351651122	18.871565414671036
17	15.648285858331231	17.747038064028235	29.313335013864382	37.29134106377615
18	21.004033274514747	22.391227627930427	36.530816738089236	20.07392235946559
19	30.786677590118476	23.941265439879004	25.644882782959417	19.627174187043106
20	32.30974287874969	22.51247794303	25.699142929165614	19.478636249054702
21	20.872699773128307	28.465528106881777	28.65811696496093	22.00365515502899
22	21.673997983362742	25.122573733299724	24.438555583564405	28.764872699773132
23	18.093458532896395	31.204940761280564	25.511784723972774	25.189815981850266
24	19.064973531636	22.91876733047643	41.45928913536678	16.556970002520796
25	15.407234686160825	25.678661457020418	38.32285102092261	20.591252835896142
26	15.157045626417947	36.19347113687925	28.865011343584573	19.784471893118223
27	16.663851777161582	36.37301487269977	29.60284850012604	17.360284850012604
28	14.011091504915555	28.812011595664227	40.47844718931182	16.698449710108395
29	14.480778926140662	24.45487774136627	39.74458028737081	21.319763045122258
30	17.628119485757498	33.29430299974792	31.38851777161583	17.689059742878747
31	29.584320645323924	26.691139400050417	25.345538190068062	18.3790017645576
32	30.738215276027226	26.12055709604235	26.893937484245022	16.247290143685404
33	29.39072346861608	27.418452230904965	24.007877489286614	19.182946811192338
34	18.70008822788001	27.24256365011344	28.286362490547013	25.770985631459542
35	19.990862112427525	26.01443156037308	31.760209226115453	22.234497101083942
36	31.178661457020418	25.049596672548525	26.04701285606252	17.72472901436854
37	19.077892614066045	34.437358205192844	29.162087219561382	17.322661961179733
38	19.977690950340307	34.59314343332493	23.672800604991178	21.756365011343583
39	19.770859591630956	33.97945550794051	27.19164355936476	19.058041341063774
40	24.478510209226116	26.587345601209982	24.661457020418453	24.27268716914545
41	16.76796067557348	24.338669019410133	26.971767078396773	31.92160322661961
42	21.02356944794555	24.947378371565414	24.80684396269221	29.222208217796826
43	21.484371061255356	25.35581043609781	26.581043609780693	26.578774892866146
44	18.46073859339551	31.725107133854298	28.44334509705067	21.37080917569952
45	16.198764809679858	40.61280564658432	22.586337282581294	20.602092261154525
46	21.220002520796573	35.130766322157804	24.895891101588102	18.753340055457524
47	21.321905722208218	28.150113435845725	26.755356692714898	23.772624149231156
48	23.152004033274515	24.32341820015125	31.548462314091253	20.976115452482986
49	20.631711620872196	24.445172674565164	32.97542223342576	21.94769347113688
50	18.574237459037054	35.64853793798841	26.142298966473405	19.634925636501137
51	18.082493067809427	35.50768842954374	23.725296193597174	22.684522309049658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1152.0
1	1621.5
2	2091.0
3	6289.0
4	10487.0
5	8063.0
6	5639.0
7	6082.0
8	6525.0
9	7247.0
10	7969.0
11	8358.5
12	8748.0
13	8636.5
14	8525.0
15	8202.5
16	7880.0
17	7178.5
18	6477.0
19	6741.5
20	7006.0
21	6871.5
22	6737.0
23	7128.0
24	7519.0
25	8086.0
26	9821.5
27	10990.0
28	15568.0
29	20146.0
30	22659.0
31	25172.0
32	28232.5
33	31293.0
34	36826.5
35	42360.0
36	45583.0
37	48806.0
38	62165.0
39	75524.0
40	98805.5
41	122087.0
42	147793.0
43	173499.0
44	176823.5
45	180148.0
46	170139.5
47	160131.0
48	144358.5
49	128586.0
50	121713.5
51	114841.0
52	102049.0
53	89257.0
54	76465.5
55	63674.0
56	57356.5
57	51039.0
58	44509.5
59	37980.0
60	35593.5
61	33207.0
62	29761.0
63	26315.0
64	22741.5
65	19168.0
66	15707.0
67	12246.0
68	10729.0
69	9212.0
70	7619.0
71	6026.0
72	4718.0
73	3410.0
74	3340.5
75	2427.0
76	1583.0
77	1294.0
78	1005.0
79	648.5
80	292.0
81	173.5
82	55.0
83	51.5
84	48.0
85	31.5
86	15.0
87	9.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1586800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.69827573050808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21752475412568	14.197118715173735
2	7.349344051516828	2.6014143492422837
3	2.6166181044219856	1.3892888608049903
4	1.3049906561876994	0.9238433783578628
5	0.83274634847876	0.7369087244475431
6	0.5218503958505966	0.5541511317503188
7	0.393681594982752	0.4877239792621665
8	0.3065279308886827	0.4340012671976024
9	0.26063796655677185	0.4151558338164622
>10	3.28031638969183	14.033638376481672
>50	1.4411896727202758	18.588097094049886
>100	1.4663162535909964	43.83839618111169
>500	0.006461124250829357	0.6952526118031224
>1k	0.0010768540418048928	0.2829545991384418
>5k	7.179026945365952E-4	0.8220548973622445
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6895	0.43452230904965966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6045	0.3809553819006806	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2016	0.1270481472145198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03888328711872952	0.0
2	0.0	0.0	0.0	0.12982102344340812	0.0
3	0.0	0.0	0.0	0.18445928913536677	0.0
4	0.0	0.0	0.0	0.27886312074615577	0.0
5	0.0	0.0	0.0	0.45878497605243257	0.0
6	0.0	0.0	0.0	0.6557222082177968	0.0
7	0.0	0.0	0.0	0.7730022687169146	0.0
8	0.0	0.0	0.0	1.0910007562389714	0.0
9	0.0	0.0	0.0	1.2289513486261658	0.0
10	0.0	0.0	0.0	1.4540584824804639	0.0
11	0.0	0.0	0.0	1.6702167885051675	0.0
12	0.0	0.0	0.0	1.867091000756239	0.0
13	1.2603982858583313E-4	0.0	0.0	1.9420216788505167	0.0
14	1.2603982858583313E-4	0.0	0.0	1.9709478195109655	0.0
15	1.2603982858583313E-4	0.0	0.0	2.0155659188303505	0.0
16	1.2603982858583313E-4	0.0	0.0	2.1167128812704816	0.0
17	1.2603982858583313E-4	0.0	0.0	2.240168893370305	0.0
18	1.2603982858583313E-4	0.0	0.0	2.4103856818754728	0.0
19	1.2603982858583313E-4	0.0	0.0	2.4844971010839427	0.0
20	2.5207965717166626E-4	0.0	0.0	2.5674313082934206	0.0
21	2.5207965717166626E-4	0.0	0.0	2.67588858079153	0.0
22	2.5207965717166626E-4	0.0	0.0	2.788001008318629	0.0
23	2.5207965717166626E-4	0.0	0.0	2.929858835391984	0.0
24	2.5207965717166626E-4	0.0	0.0	3.036236450718427	0.0
25	2.5207965717166626E-4	0.0	0.0	3.1256617091000756	0.0
26	2.5207965717166626E-4	0.0	0.0	3.210486513738341	0.0
27	2.5207965717166626E-4	0.0	0.0	3.293672800604991	0.0
28	2.5207965717166626E-4	0.0	0.0	3.3822788001008317	0.0
29	2.5207965717166626E-4	0.0	0.0	3.4807789261406605	0.0
30	2.5207965717166626E-4	0.0	0.0	3.61041088984119	0.0
31	2.5207965717166626E-4	0.0	0.0	3.734055961683892	0.0
32	3.150995714645828E-4	0.0	0.0	3.8426392740105872	0.0
33	3.150995714645828E-4	0.0	0.0	3.943471136879254	0.0
34	3.150995714645828E-4	0.0	0.0	4.060688177464079	0.0
35	3.781194857574994E-4	0.0	0.0	4.2155281068817745	0.0
36	3.781194857574994E-4	0.0	0.0	4.330539450466348	0.0
37	3.781194857574994E-4	0.0	0.0	4.45506680110915	0.0
38	4.411394000504159E-4	0.0	0.0	4.570393244265188	0.0
39	4.411394000504159E-4	0.0	0.0	4.6842072094781955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	30	2.1660271E-6	45.000004	24
CTCGGTT	30	2.1660271E-6	45.000004	22
AGGCACG	30	2.1660271E-6	45.000004	1
TGTTACG	60	0.0	45.000004	1
ACTATCG	20	7.0340995E-4	45.0	33
AACCCGC	20	7.0340995E-4	45.0	25
ATTACGA	20	7.0340995E-4	45.0	11
CGGCACT	25	3.89148E-5	45.0	31
CTAACGG	25	3.89148E-5	45.0	2
TTACGTA	40	6.8175723E-9	45.0	18
CGTAAAG	40	6.8175723E-9	45.0	30
ACCGTTC	20	7.0340995E-4	45.0	11
ACGTATG	25	3.89148E-5	45.0	1
ACGTAAG	35	1.21232E-7	45.0	1
CGGTCCA	25	3.89148E-5	45.0	34
TTCGCAC	25	3.89148E-5	45.0	44
CCACGGT	25	3.89148E-5	45.0	34
ATGCACG	35	1.21232E-7	45.0	1
CGATATC	25	3.89148E-5	45.0	10
ATCGCAA	20	7.0340995E-4	45.0	14
>>END_MODULE