FastQCFastQC Report
Thu 26 May 2016
SRR1545402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545402_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2717557
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC118460.43590622018231817No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111570.4105525661467266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG351.2127384E-745.0000041
CGTACTC207.0349884E-445.036
CGTCGAA253.8922168E-545.024
CGCAATA207.0349884E-445.033
TCGTTGC302.1666E-644.99999638
TACGCTA302.1666E-644.99999610
TACGAGT302.1666E-644.99999641
TAATGCG1300.043.269231
CGTTTTT91100.041.319981
CATAGCG556.184564E-1140.9090921
CGTTAGG2550.040.5882342
CCGGCAT501.0822987E-940.544
TTAACGG3250.040.1538432
AAATGCG850.039.7058831
GTTACCT403.4605546E-739.37527
TATTACG1150.039.1304361
CATTGCG750.039.01
GCGATGA5500.038.8636369
GTTTACG1450.038.7931061
CTTAACG700.038.571431
Produced by FastQC (version 0.11.5)