FastQCFastQC Report
Thu 26 May 2016
SRR1545401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545401_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1446077
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.411043118727426No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27810.19231341069666413No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21250.14694929799727124No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15420.10663332588790224No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15270.10559603672556855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA207.0338923E-445.09
GTCGAAC253.8913073E-545.045
TCTTACG253.8913073E-545.01
TATATCG207.0338923E-445.037
CGAATTA207.0338923E-445.044
ATCGGAC207.0338923E-445.040
ACGGGTA800.042.18755
CGAATAT2600.041.53846414
CGTAAGG2600.041.5384642
TCACGAC603.6379788E-1241.25000425
CTACGAA2750.040.09090811
GCTACGA2750.040.09090810
ATAGGGC7300.040.0684934
TAATGCG451.9288564E-840.01
ACCCGCT2800.039.37500434
CAATGCG403.4590448E-739.3751
AAATCGT356.2494073E-638.5714312
CGCGACC3100.038.4677410
TATGGGC5100.038.3823554
GCGATAT1600.037.968759