##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545401_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1446077
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266401443353292	31.0	31.0	33.0	30.0	34.0
2	31.61149164256122	31.0	31.0	34.0	30.0	34.0
3	31.71384649641755	31.0	31.0	34.0	30.0	34.0
4	35.43633706918788	37.0	35.0	37.0	33.0	37.0
5	35.29037319589482	37.0	35.0	37.0	33.0	37.0
6	35.36091093351184	37.0	35.0	37.0	33.0	37.0
7	35.80165509858742	37.0	35.0	37.0	35.0	37.0
8	35.818629298439845	37.0	35.0	37.0	35.0	37.0
9	37.4420158815886	39.0	37.0	39.0	35.0	39.0
10	36.83882877606103	39.0	37.0	39.0	32.0	39.0
11	36.49647494566334	39.0	35.0	39.0	32.0	39.0
12	35.851894470349784	37.0	35.0	39.0	31.0	39.0
13	35.548151308678584	37.0	35.0	39.0	30.0	39.0
14	36.56542493933587	38.0	35.0	41.0	31.0	41.0
15	36.75065712268434	38.0	35.0	41.0	31.0	41.0
16	36.9052927333745	38.0	35.0	41.0	32.0	41.0
17	36.83938822068258	38.0	35.0	41.0	32.0	41.0
18	36.753237206594115	38.0	35.0	40.0	31.0	41.0
19	36.706410516175836	38.0	35.0	40.0	31.0	41.0
20	36.55436951144372	38.0	35.0	40.0	31.0	41.0
21	36.36618589466536	38.0	35.0	40.0	31.0	41.0
22	36.287018602743835	38.0	35.0	40.0	30.0	41.0
23	36.214037703386474	38.0	35.0	40.0	30.0	41.0
24	36.13431719057837	38.0	35.0	40.0	30.0	41.0
25	36.00785158743276	37.0	34.0	40.0	30.0	41.0
26	35.91664759207151	37.0	34.0	40.0	30.0	41.0
27	35.88443008221554	37.0	34.0	40.0	30.0	41.0
28	35.846245393571714	37.0	34.0	40.0	30.0	41.0
29	35.83813448384837	37.0	34.0	40.0	30.0	41.0
30	35.75156509646443	37.0	34.0	40.0	30.0	41.0
31	35.59956212566827	37.0	34.0	40.0	29.0	41.0
32	35.433989338050466	37.0	34.0	40.0	29.0	41.0
33	35.269292022485665	37.0	34.0	40.0	27.0	41.0
34	35.09973189532784	37.0	34.0	40.0	27.0	41.0
35	34.960670835647065	37.0	34.0	40.0	26.0	41.0
36	34.79597628618669	37.0	34.0	40.0	25.0	41.0
37	34.72321944128839	37.0	33.0	40.0	25.0	41.0
38	34.67175330221005	37.0	33.0	40.0	25.0	41.0
39	34.58513896562908	36.0	33.0	40.0	24.0	41.0
40	34.41929648282906	36.0	33.0	40.0	24.0	41.0
41	34.37738101083137	36.0	33.0	40.0	24.0	41.0
42	34.28550761819737	36.0	33.0	40.0	24.0	41.0
43	34.21605557657026	36.0	33.0	40.0	23.0	41.0
44	34.164708379982535	36.0	33.0	40.0	23.0	41.0
45	34.11660236626404	35.0	33.0	40.0	23.0	41.0
46	34.071961589873844	35.0	33.0	40.0	23.0	41.0
47	34.008629554304505	35.0	33.0	40.0	23.0	41.0
48	33.94295670285884	35.0	33.0	40.0	23.0	41.0
49	33.86700639039277	35.0	33.0	40.0	24.0	41.0
50	33.74381723794791	35.0	33.0	39.0	24.0	41.0
51	33.58925008834246	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	1.0
13	8.0
14	16.0
15	52.0
16	140.0
17	436.0
18	936.0
19	1744.0
20	2989.0
21	4658.0
22	6874.0
23	9649.0
24	13516.0
25	18648.0
26	24399.0
27	28843.0
28	31848.0
29	35779.0
30	42366.0
31	51031.0
32	63099.0
33	79549.0
34	129168.0
35	187720.0
36	108383.0
37	131140.0
38	182531.0
39	290405.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.78030561304827	23.378768903730577	27.73358541765065	14.107340065570506
2	31.81504166099039	25.342979661525632	28.923217781625738	13.918760895858243
3	29.643857139004353	25.421398722198056	30.798774892346675	14.135969246450916
4	27.003472152589385	27.579651705960334	30.56282618422117	14.85404995722911
5	24.026175646248436	31.711658507811137	29.299546289720396	14.962619556220035
6	22.478540216046586	40.5444523355257	26.48641808147146	10.490589366956254
7	85.18495211527464	5.0697162046004465	7.53528339085678	2.210048289268137
8	86.04804585094708	4.4565400044395975	7.28211568263654	2.213298461976783
9	80.49350069187187	6.7959036759453335	9.188445705173375	3.522149927009419
10	46.75076085160057	24.412877045966432	15.478013964678231	13.358348137754767
11	40.85480925289594	22.660757345563205	22.101451029232884	14.382982372307977
12	37.088896372738105	22.611174923603652	25.730165129519385	14.569763574138861
13	22.652251574432068	34.64926141554011	25.860863563973425	16.837623446054394
14	17.24105977759137	37.03848411944869	28.57033200859982	17.15012409436012
15	17.0188724390195	23.757448600593193	42.748830110706415	16.474848849680896
16	18.430553836344814	20.05114527096413	41.4612084971962	20.05709239549485
17	18.537809535730116	20.841559612662397	30.5013495132002	30.11928133840729
18	21.71108454114131	23.706828889471307	33.740319498892525	20.841767070494864
19	29.38591790063738	23.96193287079457	26.548655431211476	20.103493797356574
20	31.437053490235993	21.89406234937697	27.167363840238107	19.501520320148927
21	23.97583254556984	26.396104771737605	28.004801957295495	21.623260725397056
22	24.06427873481149	25.118233676353334	25.858581527816295	24.958906061018883
23	22.378061472521864	28.484859381623522	25.925936170757158	23.211142975097452
24	21.07453475852254	23.270060999518005	36.32641968581203	19.328984556147425
25	19.586370573627825	25.486333023760142	33.57808747390354	21.3492089287085
26	18.973194373466974	31.870087139204895	28.093248146537146	21.063470340790982
27	20.170087761578394	31.76829449607455	27.866842498705118	20.194775243641935
28	18.26223638160347	28.47891225709281	34.26076204794074	18.998089313362982
29	18.610488929704296	25.1134621462066	34.31739803620415	21.958650887884946
30	20.059305279041155	29.67214055683065	30.069007390339518	20.199546773788672
31	26.876992027395495	27.55178320379897	26.06569359722892	19.505531171576617
32	28.472135301232232	26.368720337851997	26.9749121243198	18.184232236595975
33	26.88439135675348	27.83109059891002	25.238005998297464	20.04651204603904
34	21.10503105989515	27.864007241661405	27.99546635483449	23.03549534360895
35	22.2491610059492	25.558044281182813	29.763975224002593	22.428819488865393
36	27.13707499669796	27.435675970228417	25.78161467197113	19.645634361102488
37	21.845517216579754	31.60011534655485	28.05576743147149	18.498600005393904
38	21.3479641817137	31.73005310228985	25.555485634582393	21.36649708141406
39	21.95567732561959	31.27399163391714	27.14212313728799	19.62820790317528
40	24.388742784789468	27.080992229321122	27.326553150350914	21.203711835538495
41	19.39716903041816	25.65278335800929	28.516116361715177	26.433931249857373
42	21.70008927602057	26.378055940313	25.602094494276585	26.319760289389844
43	22.31478683361951	26.731909849890428	27.112456667245244	23.840846649244817
44	20.87613591807352	29.743367745977565	27.49521636814637	21.885279967802546
45	18.672380516390206	33.92205256013338	24.97522607717293	22.43034084630348
46	21.053996433108335	32.75157546935606	25.857060170378205	20.337367927157405
47	22.69270585176308	28.575172691357377	26.834048256074883	21.89807320080466
48	23.323239357240315	25.169475760972617	29.994115112819024	21.513169768968044
49	21.872694192632895	26.172603533560107	30.46670405517825	21.487998218628746
50	20.305972641844107	31.783784680898737	26.981481622347914	20.92876105490925
51	20.093120905733237	31.926377364414204	25.83873472851031	22.141767001342252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	902.0
1	1739.5
2	2577.0
3	6966.5
4	11356.0
5	8825.0
6	6294.0
7	6587.0
8	6880.0
9	6989.5
10	7099.0
11	7377.5
12	7656.0
13	7265.5
14	6875.0
15	6651.5
16	6428.0
17	6089.0
18	5750.0
19	5976.5
20	6203.0
21	6409.5
22	6616.0
23	7020.5
24	7425.0
25	8218.0
26	10495.0
27	11979.0
28	15216.0
29	18453.0
30	21152.5
31	23852.0
32	27782.5
33	31713.0
34	36397.0
35	41081.0
36	44746.5
37	48412.0
38	54524.0
39	60636.0
40	76013.5
41	91391.0
42	107388.0
43	123385.0
44	129370.5
45	135356.0
46	128090.0
47	120824.0
48	113326.0
49	105828.0
50	99650.0
51	93472.0
52	87767.5
53	82063.0
54	76817.5
55	71572.0
56	65339.0
57	59106.0
58	57263.0
59	55420.0
60	53339.0
61	51258.0
62	44702.0
63	38146.0
64	33201.0
65	28256.0
66	24731.0
67	21206.0
68	17973.0
69	14740.0
70	12404.5
71	10069.0
72	8985.0
73	7901.0
74	6125.5
75	3336.5
76	2323.0
77	1714.0
78	1105.0
79	893.0
80	681.0
81	473.0
82	265.0
83	183.5
84	102.0
85	68.5
86	35.0
87	24.0
88	13.0
89	10.5
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1446077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.75654172073854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.02878801483945	14.635502178189993
2	6.141391595806544	2.3038253538027695
3	2.313503441826853	1.3017997148309273
4	1.2801683497161316	0.9604612424407851
5	0.8931874812974302	0.8376554128698313
6	0.6318614480574126	0.7110921367329075
7	0.5099793507545018	0.6695814278399376
8	0.45317893831485456	0.6800055730770053
9	0.35238668766379266	0.5948600048098909
>10	6.582333473021975	31.399649975914357
>50	2.1673020737948923	28.830854852453975
>100	0.6410905371523636	15.615174250595581
>500	0.0022285881940406443	0.32165567424978964
>1k	0.0022285881940406443	0.7237775178213266
>5k	3.714313656734407E-4	0.4141046843709659
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5944	0.411043118727426	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2781	0.19231341069666413	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2125	0.14694929799727124	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1542	0.10663332588790224	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1527	0.10559603672556855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019016967976117453	0.0
2	0.0	0.0	0.0	0.060646839691109115	0.0
3	0.0	0.0	0.0	0.08893025751740744	0.0
4	0.0	0.0	0.0	0.1470184506080935	0.0
5	0.0	0.0	0.0	0.2574551700912192	0.0
6	0.0	0.0	0.0	0.4035746367586235	0.0
7	0.0	0.0	0.0	0.4838608179232503	0.0
8	0.0	0.0	0.0	0.682259658372272	0.0
9	0.0	0.0	0.0	0.791382478249775	0.0
10	0.0	0.0	0.0	1.1222777210342187	0.0
11	0.0	0.0	0.0	1.2697110873072457	0.0
12	0.0	0.0	0.0	1.3748922083678807	0.0
13	0.0	0.0	0.0	1.419772252791518	0.0
14	0.0	0.0	0.0	1.4378902368269464	0.0
15	6.915261082224529E-5	0.0	0.0	1.466796028150645	0.0
16	6.915261082224529E-5	0.0	0.0	1.5320069401560221	0.0
17	6.915261082224529E-5	0.0	0.0	1.6048246393518464	0.0
18	6.915261082224529E-5	0.0	0.0	1.701430836670523	0.0
19	6.915261082224529E-5	0.0	0.0	1.7490078329162277	0.0
20	6.915261082224529E-5	0.0	0.0	1.799489238816467	0.0
21	6.915261082224529E-5	0.0	0.0	1.869125917914468	0.0
22	6.915261082224529E-5	0.0	0.0	1.9474758259760718	0.0
23	6.915261082224529E-5	0.0	0.0	2.033294216006478	0.0
24	6.915261082224529E-5	0.0	0.0	2.1023776742179012	0.0
25	6.915261082224529E-5	0.0	0.0	2.1600509516436537	0.0
26	6.915261082224529E-5	0.0	0.0	2.220628638723941	0.0
27	6.915261082224529E-5	0.0	0.0	2.274844285608581	0.0
28	6.915261082224529E-5	0.0	0.0	2.3321026473694	0.0
29	6.915261082224529E-5	0.0	0.0	2.397728475039711	0.0
30	6.915261082224529E-5	0.0	0.0	2.487142800832874	0.0
31	6.915261082224529E-5	0.0	0.0	2.5688120342139458	0.0
32	6.915261082224529E-5	0.0	0.0	2.6454331270049933	0.0
33	6.915261082224529E-5	0.0	0.0	2.719218962752329	0.0
34	1.3830522164449057E-4	0.0	0.0	2.798951923030378	0.0
35	1.3830522164449057E-4	0.0	0.0	2.895350662516588	0.0
36	1.3830522164449057E-4	0.0	0.0	2.9846266830881065	0.0
37	1.3830522164449057E-4	0.0	0.0	3.0777060972548487	0.0
38	1.3830522164449057E-4	0.0	0.0	3.163731945117722	0.0
39	1.3830522164449057E-4	0.0	0.0	3.255635764900486	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.0338923E-4	45.0	9
GTCGAAC	25	3.8913073E-5	45.0	45
TCTTACG	25	3.8913073E-5	45.0	1
TATATCG	20	7.0338923E-4	45.0	37
CGAATTA	20	7.0338923E-4	45.0	44
ATCGGAC	20	7.0338923E-4	45.0	40
ACGGGTA	80	0.0	42.1875	5
CGAATAT	260	0.0	41.538464	14
CGTAAGG	260	0.0	41.538464	2
TCACGAC	60	3.6379788E-12	41.250004	25
CTACGAA	275	0.0	40.090908	11
GCTACGA	275	0.0	40.090908	10
ATAGGGC	730	0.0	40.068493	4
TAATGCG	45	1.9288564E-8	40.0	1
ACCCGCT	280	0.0	39.375004	34
CAATGCG	40	3.4590448E-7	39.375	1
AAATCGT	35	6.2494073E-6	38.57143	12
CGCGACC	310	0.0	38.46774	10
TATGGGC	510	0.0	38.382355	4
GCGATAT	160	0.0	37.96875	9
>>END_MODULE