FastQCFastQC Report
Thu 26 May 2016
SRR1545400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545400_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1267555
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.4162344040298054No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30050.23707058076375384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG750.045.0000041
TCTCGAC207.0335617E-445.036
GTCGTAC207.0335617E-445.010
TTGCGCA207.0335617E-445.02
TCATACG207.0335617E-445.01
CGACTAT207.0335617E-445.031
CAACGAA253.8910348E-544.99999614
CGTTATA253.8910348E-544.99999619
GCGATCG253.8910348E-544.9999969
ACTCGAT302.1656797E-644.99999640
TATACGA253.8910348E-544.99999615
TATCCGA302.1656797E-644.99999631
CATACGA2000.040.49999618
CGTTTTT44650.040.1623731
TATTGCG800.039.3751
CGTACAT403.45859E-739.37535
ACTCGAC356.2487998E-638.57142641
TTACGCC356.2487998E-638.57142638
CATTCTA2400.038.43749645
GTTAGCG1300.038.076921
Produced by FastQC (version 0.11.5)