##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545400_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1267555
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333500321485065	31.0	31.0	33.0	30.0	34.0
2	31.689620568732717	31.0	31.0	34.0	30.0	34.0
3	31.786355621649555	31.0	31.0	34.0	30.0	34.0
4	35.49763284433417	37.0	35.0	37.0	33.0	37.0
5	35.3555498577971	37.0	35.0	37.0	33.0	37.0
6	35.42018926200441	37.0	35.0	37.0	33.0	37.0
7	35.83493733999708	37.0	35.0	37.0	35.0	37.0
8	35.857999850105124	37.0	35.0	37.0	35.0	37.0
9	37.50416668310251	39.0	37.0	39.0	35.0	39.0
10	36.92609078107064	39.0	37.0	39.0	32.0	39.0
11	36.57460780794522	39.0	35.0	39.0	32.0	39.0
12	35.83020302866542	37.0	35.0	39.0	31.0	39.0
13	35.49783401903665	37.0	35.0	39.0	30.0	39.0
14	36.49879334624533	38.0	35.0	41.0	31.0	41.0
15	36.71180816611508	38.0	35.0	41.0	31.0	41.0
16	36.876053504581655	38.0	35.0	41.0	32.0	41.0
17	36.834174453968465	38.0	35.0	41.0	32.0	41.0
18	36.720347440544984	38.0	35.0	40.0	31.0	41.0
19	36.68093692186927	38.0	35.0	40.0	31.0	41.0
20	36.52100540016015	38.0	35.0	40.0	31.0	41.0
21	36.31789153133395	38.0	35.0	40.0	31.0	41.0
22	36.2609748689406	38.0	35.0	40.0	31.0	41.0
23	36.19428979413122	37.0	35.0	40.0	31.0	41.0
24	36.12200180662772	37.0	35.0	40.0	30.0	41.0
25	35.997579592207046	37.0	34.0	40.0	30.0	41.0
26	35.88950459743364	37.0	34.0	40.0	30.0	41.0
27	35.85415070746437	37.0	34.0	40.0	30.0	41.0
28	35.82879875035008	37.0	34.0	40.0	30.0	41.0
29	35.850846708821315	37.0	35.0	40.0	30.0	41.0
30	35.76530249180509	37.0	34.0	40.0	30.0	41.0
31	35.59356872088391	37.0	34.0	40.0	29.0	41.0
32	35.41214542958688	37.0	34.0	40.0	29.0	41.0
33	35.235939268907465	37.0	34.0	40.0	28.0	41.0
34	35.05035600033135	37.0	34.0	40.0	27.0	41.0
35	34.90476784044874	36.0	34.0	40.0	26.0	41.0
36	34.74980257266943	36.0	34.0	40.0	25.0	41.0
37	34.67447960837991	36.0	33.0	40.0	25.0	41.0
38	34.62748362004016	36.0	33.0	40.0	25.0	41.0
39	34.55837103715421	36.0	33.0	40.0	25.0	41.0
40	34.3875942266805	36.0	33.0	40.0	24.0	41.0
41	34.34637944704569	36.0	33.0	40.0	24.0	41.0
42	34.26299608301021	36.0	33.0	40.0	24.0	41.0
43	34.181254462331026	35.0	33.0	40.0	23.0	41.0
44	34.094500830338724	35.0	33.0	40.0	23.0	41.0
45	34.06595532343764	35.0	33.0	40.0	23.0	41.0
46	34.02743312913444	35.0	33.0	40.0	23.0	41.0
47	33.975820378602904	35.0	33.0	40.0	23.0	41.0
48	33.94169483769935	35.0	33.0	40.0	23.0	41.0
49	33.87286389939687	35.0	33.0	39.0	24.0	41.0
50	33.75239259834879	35.0	33.0	39.0	24.0	41.0
51	33.58007897093223	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	2.0
14	14.0
15	34.0
16	102.0
17	323.0
18	772.0
19	1411.0
20	2504.0
21	3845.0
22	5855.0
23	8243.0
24	11588.0
25	16138.0
26	20944.0
27	24890.0
28	27693.0
29	31182.0
30	36969.0
31	44594.0
32	54798.0
33	70316.0
34	117928.0
35	176273.0
36	92102.0
37	112185.0
38	154852.0
39	251895.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.687382401552593	24.645163326246198	28.793543475431044	14.87391079677016
2	31.310278449455843	25.95390338091838	28.832279467163165	13.903538702462615
3	28.734690013451093	25.638571896288525	31.237697772483248	14.389040317777138
4	26.676160008835907	27.25696320869706	31.1533621815227	14.913514600944339
5	23.141086580069505	31.53046613361945	29.96958711850768	15.358860167803371
6	22.13702758460185	40.10390081692707	27.217674972683632	10.541396625787442
7	84.35452505019506	5.504139859808845	7.892675268528782	2.248659821467313
8	85.56086323670374	4.3441113008902965	7.569770148040914	2.525255314365057
9	80.58537893819204	6.1310949031797435	9.659304724449827	3.6242214341783985
10	49.805807243078206	21.65144707724714	15.860771327476913	12.681974352197736
11	42.86606892797551	22.21875973823621	20.814796991057587	14.10037434273069
12	37.5558457029478	21.954708079728295	25.389036373175127	15.100409844148773
13	21.224641139832194	36.68921664148696	26.146636635096705	15.939505583584143
14	17.56547053185069	38.220353357448	28.53469869157551	15.679477419125798
15	16.595729573864645	23.081680873808235	44.83489868289739	15.487690869429729
16	17.54006729491028	19.65106050625022	43.04531164328175	19.763560555557746
17	18.595721684660628	19.591970368149706	30.019683564026806	31.79262438316286
18	22.320293793957656	22.649194709499785	34.19188910934831	20.838622387194246
19	28.92458315418266	23.762755856747834	26.82400369214748	20.488657296922028
20	31.2220771485261	22.417646571549163	26.807041903507145	19.553234376417592
21	23.352832815933038	26.14466433409201	28.724512940266894	21.777989909708058
22	23.345732532316152	23.644812256667365	27.413248340308705	25.59620687070778
23	21.50486566657857	27.270769315729886	27.118586570208	24.10577844748354
24	20.811246849249144	22.685642832066456	37.090540449921306	19.41256986876309
25	19.28381805917692	24.36706888458489	34.722516971650144	21.626596084588044
26	18.52353546788897	32.46849249145007	27.831060585142264	21.176911455518695
27	18.72344789772436	32.38384133232877	29.169779615085734	19.722931154861133
28	17.417390172418553	27.860960668373362	36.601252016677776	18.120397142530305
29	18.336876900805095	24.38892197971686	35.56958080714446	21.704620312333585
30	19.409493079195776	29.223268418332932	31.28590080903787	20.081337693433422
31	27.665781760949226	26.293454721885833	26.59695240048755	19.443811116677384
32	28.66163598423737	25.649774565995166	27.687871532201758	18.000717917565705
33	27.130657052356703	27.35084473652031	25.672337689488817	19.84616052163417
34	20.63421311106816	27.186591508849716	28.44192165231489	23.737273727767235
35	21.614210034278592	26.56302882320688	29.405114570965363	22.417646571549163
36	28.39356083167989	26.14766223161914	26.468595051102316	18.990181885598652
37	22.092532473936043	31.968080280540097	28.027580657249583	17.911806588274278
38	21.560721231031398	31.854712418790506	24.987949240861344	21.596617109316753
39	21.968593078801316	30.07617026480113	27.78388314511007	20.171353511287478
40	24.094102425535777	26.715606028929713	26.72144403990359	22.468847505630922
41	19.437341969382	25.439053926654072	28.588976415224586	26.53462768873934
42	22.531803353700628	25.576957212902002	26.06892797551191	25.822311457885455
43	22.84374248060242	25.695689733384352	27.18596037252821	24.274607413485015
44	20.707109356201507	29.321015656125375	28.65690246182611	21.314972525847004
45	18.831687776861752	34.31772191344754	25.078517302996715	21.77207300669399
46	22.021608529807384	31.76201427157007	25.927395655415346	20.2889815432072
47	22.044329437381414	27.54547139966313	27.770629282358556	22.639569880596895
48	23.588956692214538	24.404621495714192	30.311505220680758	21.694916591390513
49	22.45070233638777	24.85359609642185	31.35327461135809	21.34242695583229
50	20.431618351866387	31.336233930677565	27.475809728177474	20.756337989278574
51	20.04425843454525	32.27757375419607	25.14028977046361	22.53787804079507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	769.0
1	1548.0
2	2327.0
3	6306.0
4	10285.0
5	7788.5
6	5292.0
7	5560.0
8	5828.0
9	6040.0
10	6252.0
11	6459.5
12	6667.0
13	6549.5
14	6432.0
15	6377.0
16	6322.0
17	6015.0
18	5708.0
19	5468.0
20	5228.0
21	5613.5
22	5999.0
23	6444.0
24	6889.0
25	7119.0
26	8739.5
27	10130.0
28	12608.5
29	15087.0
30	18218.0
31	21349.0
32	24705.5
33	28062.0
34	30778.5
35	33495.0
36	35599.0
37	37703.0
38	46000.5
39	54298.0
40	69024.5
41	83751.0
42	97764.5
43	111778.0
44	112728.5
45	113679.0
46	112349.5
47	111020.0
48	103930.0
49	96840.0
50	92484.5
51	88129.0
52	81506.0
53	74883.0
54	68077.5
55	61272.0
56	55829.5
57	50387.0
58	47167.0
59	43947.0
60	41840.5
61	39734.0
62	35511.0
63	31288.0
64	28278.5
65	25269.0
66	21697.0
67	18125.0
68	15205.0
69	12285.0
70	10366.5
71	8448.0
72	7284.5
73	6121.0
74	5072.5
75	3257.0
76	2490.0
77	1927.0
78	1364.0
79	953.5
80	543.0
81	472.5
82	402.0
83	287.0
84	172.0
85	122.5
86	73.0
87	51.5
88	30.0
89	22.0
90	14.0
91	10.5
92	7.0
93	6.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1267555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.500479080037337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47037653529587	14.702400385739312
2	6.001768926297222	2.220712009283598
3	2.1157244105576942	1.1742574558994083
4	1.1615794051850963	0.8595910194171635
5	0.7632429096298158	0.7060179741296617
6	0.5647472569181627	0.6268856887273765
7	0.42834709096782886	0.5547238476811618
8	0.35761925361298325	0.529290201606845
9	0.3111817139476417	0.5181309710080658
>10	5.714190835211965	27.14075342823555
>50	2.258608688613205	29.61048701206723
>100	0.8500371037511177	20.488558441835846
>500	0.0017172466742446824	0.21047549589034426
>1k	4.293116685611706E-4	0.23867126986810738
>5k	4.293116685611706E-4	0.4190447986103609
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5276	0.4162344040298054	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3005	0.23707058076375384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009545936862700237	0.0
2	0.0	0.0	0.0	0.05009644551912935	0.0
3	0.0	0.0	0.0	0.0806276650717326	0.0
4	0.0	0.0	0.0	0.15226163756207817	0.0
5	0.0	0.0	0.0	0.275017652093992	0.0
6	0.0	0.0	0.0	0.4370619026393332	0.0
7	0.0	0.0	0.0	0.5213974935998832	0.0
8	0.0	0.0	0.0	0.74750208077756	0.0
9	0.0	0.0	0.0	0.8723092883543515	0.0
10	0.0	0.0	0.0	1.0767974565206244	0.0
11	0.0	0.0	0.0	1.2599058817960562	0.0
12	0.0	0.0	0.0	1.4133508999609485	0.0
13	0.0	0.0	0.0	1.482775895326041	0.0
14	0.0	0.0	0.0	1.5084947004272005	0.0
15	0.0	0.0	0.0	1.5433649821901219	0.0
16	0.0	0.0	0.0	1.6140522501982162	0.0
17	0.0	0.0	0.0	1.696021079953138	0.0
18	0.0	0.0	0.0	1.8034720386886565	0.0
19	0.0	0.0	0.0	1.8579864384582918	0.0
20	0.0	0.0	0.0	1.9151831675943056	0.0
21	0.0	0.0	0.0	1.993049611259472	0.0
22	0.0	0.0	0.0	2.074229520612518	0.0
23	0.0	0.0	0.0	2.161957469301135	0.0
24	0.0	0.0	0.0	2.2313824646662277	0.0
25	0.0	0.0	0.0	2.2897625744050556	0.0
26	0.0	0.0	0.0	2.3449870025363793	0.0
27	0.0	0.0	0.0	2.402735975953706	0.0
28	0.0	0.0	0.0	2.456540347361653	0.0
29	0.0	0.0	0.0	2.520127331752863	0.0
30	0.0	0.0	0.0	2.598782695819905	0.0
31	0.0	0.0	0.0	2.680041497213139	0.0
32	0.0	0.0	0.0	2.7592491055614943	0.0
33	0.0	0.0	0.0	2.834591003940657	0.0
34	0.0	0.0	0.0	2.9109584988422594	0.0
35	0.0	0.0	0.0	3.002157697299131	0.0
36	0.0	0.0	0.0	3.0869666405008065	0.0
37	0.0	0.0	0.0	3.1746156971492363	0.0
38	0.0	0.0	0.0	3.25816236770791	0.0
39	0.0	0.0	0.0	3.3459692084367147	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGTG	75	0.0	45.000004	1
TCTCGAC	20	7.0335617E-4	45.0	36
GTCGTAC	20	7.0335617E-4	45.0	10
TTGCGCA	20	7.0335617E-4	45.0	2
TCATACG	20	7.0335617E-4	45.0	1
CGACTAT	20	7.0335617E-4	45.0	31
CAACGAA	25	3.8910348E-5	44.999996	14
CGTTATA	25	3.8910348E-5	44.999996	19
GCGATCG	25	3.8910348E-5	44.999996	9
ACTCGAT	30	2.1656797E-6	44.999996	40
TATACGA	25	3.8910348E-5	44.999996	15
TATCCGA	30	2.1656797E-6	44.999996	31
CATACGA	200	0.0	40.499996	18
CGTTTTT	4465	0.0	40.162373	1
TATTGCG	80	0.0	39.375	1
CGTACAT	40	3.45859E-7	39.375	35
ACTCGAC	35	6.2487998E-6	38.571426	41
TTACGCC	35	6.2487998E-6	38.571426	38
CATTCTA	240	0.0	38.437496	45
GTTAGCG	130	0.0	38.07692	1
>>END_MODULE