FastQCFastQC Report
Thu 26 May 2016
SRR1545399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545399_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3601833
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249240.6919809996743325No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59090.1640553573694283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA207.0352934E-445.025
CGTTTTT191750.042.1955681
CGCATTA556.184564E-1140.90909245
GTCCGAT403.4609548E-739.37538
GCGATCG1600.037.968759
AATACGG2100.037.52
ACGGGTA4750.037.421055
AGGGCGA38250.037.4117666
TAGTACG1150.037.1739121
ATACTAT26550.037.033945
ACGTAGG2950.036.610172
GGGCGAT70200.036.057697
CGACGTC1000.036.018
ATCGTAC504.8839865E-836.028
CGTTAGG4000.036.02
CGAATCG250.002107704336.022
TCGTACT504.8839865E-836.029
CGGTAGT15650.035.7987212
GCTACGA3900.035.7692310
TAGGGCG16950.035.442485
Produced by FastQC (version 0.11.5)