##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545399_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3601833
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252427028127066	31.0	31.0	33.0	30.0	34.0
2	31.60242743069987	31.0	31.0	34.0	30.0	34.0
3	31.67866722305004	31.0	31.0	34.0	30.0	34.0
4	35.41738609202592	37.0	35.0	37.0	33.0	37.0
5	35.28348954546199	37.0	35.0	37.0	33.0	37.0
6	35.34975747071005	37.0	35.0	37.0	33.0	37.0
7	35.813006599695214	37.0	35.0	37.0	35.0	37.0
8	35.8354249072625	37.0	35.0	37.0	35.0	37.0
9	37.485323445034794	39.0	37.0	39.0	35.0	39.0
10	36.83671425077176	39.0	37.0	39.0	32.0	39.0
11	36.51352214275342	39.0	35.0	39.0	32.0	39.0
12	35.96746351094012	37.0	35.0	39.0	31.0	39.0
13	35.72912514267041	37.0	35.0	39.0	30.0	39.0
14	36.7257501944149	39.0	35.0	41.0	31.0	41.0
15	36.87348580569949	39.0	35.0	41.0	31.0	41.0
16	36.97978390447308	38.0	35.0	41.0	32.0	41.0
17	36.92352949178932	38.0	35.0	41.0	31.0	41.0
18	36.78356492374855	38.0	35.0	40.0	31.0	41.0
19	36.73851869312097	38.0	35.0	40.0	31.0	41.0
20	36.58268081834999	38.0	35.0	40.0	31.0	41.0
21	36.397411262543265	38.0	35.0	40.0	31.0	41.0
22	36.365130198984794	38.0	35.0	40.0	30.0	41.0
23	36.2944808934784	38.0	35.0	40.0	30.0	41.0
24	36.22341513335016	38.0	35.0	40.0	30.0	41.0
25	36.1199989005598	38.0	35.0	40.0	30.0	41.0
26	36.042780439848265	38.0	35.0	40.0	30.0	41.0
27	35.98883540686089	38.0	34.0	40.0	30.0	41.0
28	35.942211646125735	38.0	35.0	40.0	30.0	41.0
29	35.90208374458227	38.0	35.0	40.0	30.0	41.0
30	35.77327321949685	37.0	34.0	40.0	29.0	41.0
31	35.58650886923408	37.0	34.0	40.0	29.0	41.0
32	35.37812052918611	37.0	34.0	40.0	27.0	41.0
33	35.1400470260559	37.0	34.0	40.0	27.0	41.0
34	34.882573400821194	37.0	34.0	40.0	25.0	41.0
35	34.7207177567644	37.0	33.0	40.0	24.0	41.0
36	34.58852784124083	37.0	33.0	40.0	24.0	41.0
37	34.54054449498353	37.0	33.0	40.0	24.0	41.0
38	34.50229785778519	37.0	33.0	40.0	24.0	41.0
39	34.424133212172805	37.0	33.0	40.0	23.0	41.0
40	34.29636215782353	37.0	33.0	40.0	23.0	41.0
41	34.212793041765124	36.0	33.0	40.0	23.0	41.0
42	34.10964111884143	36.0	33.0	40.0	23.0	41.0
43	34.04343010905836	36.0	33.0	40.0	23.0	41.0
44	33.99260015664247	36.0	33.0	40.0	23.0	41.0
45	33.951672662225036	36.0	33.0	40.0	23.0	41.0
46	33.91234712991968	36.0	33.0	40.0	23.0	41.0
47	33.84727970452822	36.0	33.0	40.0	23.0	41.0
48	33.79224828025064	35.0	33.0	40.0	23.0	41.0
49	33.72032101432798	36.0	33.0	40.0	23.0	41.0
50	33.59982458931327	35.0	33.0	40.0	22.0	41.0
51	33.43870912393773	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	8.0
14	38.0
15	147.0
16	438.0
17	1130.0
18	2373.0
19	4675.0
20	7935.0
21	12578.0
22	18361.0
23	26501.0
24	36932.0
25	52072.0
26	68320.0
27	77302.0
28	82371.0
29	90873.0
30	105439.0
31	125943.0
32	153210.0
33	193167.0
34	300174.0
35	421552.0
36	274827.0
37	332979.0
38	466460.0
39	745717.0
40	303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.47764874162683	22.781372706619102	27.167417256713456	16.57356129504061
2	33.277084195741445	24.257870922943955	29.005509139374315	13.459535741940284
3	27.989498680255302	24.98669427483173	32.88886519724818	14.134941847664786
4	25.240537248673107	27.110668373575344	32.012616909223716	15.636177468527828
5	22.925493769422403	30.765085443994767	31.701636361263834	14.607784425318998
6	21.901376326997948	38.65923267403014	29.181947080833563	10.257443918138348
7	83.46897260367152	4.955782236433505	9.349378497004164	2.2258666628908115
8	84.3768436793155	4.041692105103151	9.212587035545512	2.368877180035832
9	79.13959364579091	5.770256422216133	11.232336424259536	3.8578135077334235
10	44.251107699885026	23.516609459683444	18.98941455642169	13.242868284009838
11	37.112159281121585	23.477712598002185	24.243683702159426	15.166444418716804
12	33.289022561567954	22.680923851827668	28.567398877182814	15.462654709421564
13	23.361521758504626	31.77021255566263	28.597494664522205	16.270771021310537
14	18.76536196986368	33.322561040448015	31.21871558176073	16.693361407927572
15	17.926122615901406	24.23690937364392	41.87281864539528	15.964149365059402
16	20.46169269924508	21.48164559545098	39.4563823475436	18.600279357760343
17	20.819732619474586	21.858425973663962	30.812228107188755	26.509613299672697
18	22.297480199665003	23.402306547804965	34.794394965008095	19.505818287521937
19	27.47315047643797	25.294926222287373	28.095944481601453	19.135978819673205
20	28.649468201329714	24.18576874608012	28.82712774301307	18.337635309577095
21	23.668698687584904	26.9661863834331	30.459990788023767	18.90512414095823
22	23.401112711222314	24.779577509562493	29.102987284529846	22.716322494685347
23	21.272085629733528	27.766140184733718	29.280036026101154	21.681738159431603
24	20.76623208238694	23.402584184219535	36.798596714506196	19.03258701888733
25	19.79755863195212	25.816577281622994	33.20956301971801	21.176301066706866
26	18.749980912496497	30.372063335529436	29.575968680391345	21.30198707158272
27	19.097553939896713	29.807517450142747	31.03222720209404	20.062701407866495
28	17.944918601167796	27.088707333182853	34.91408402332923	20.05229004232012
29	19.36427924337414	25.283793002063113	34.464923831837844	20.8870039227249
30	20.922541383789863	28.349315473538056	31.53039022075704	19.197752921915036
31	25.392737531140398	26.94677959805466	28.741032690854908	18.919450179950044
32	27.134211941530882	25.39840131399762	28.67837015208645	18.789016592385043
33	25.877879401960058	27.33791377890091	27.58159525996902	19.20261155917001
34	20.217566999913654	27.096592207356647	30.57079548107866	22.11504531165104
35	20.868985319419306	27.617216011958355	30.864007298506067	20.649791370116272
36	25.642332667838847	27.890215898405064	26.875454803151616	19.591996630604473
37	21.147177006818474	31.664377554428537	28.547186946202114	18.64125849255088
38	20.737496713478944	31.319192200193623	27.205425681868096	20.73788540445934
39	20.63230027599836	31.009821943438244	28.137312307372387	20.22056547319101
40	23.597651529096435	26.84930145289912	27.441055706913676	22.11199131109077
41	18.795402229920157	25.939709031484803	28.714934867885322	26.549953870709718
42	20.978040903062414	26.865682001358753	27.170221384500614	24.98605571107822
43	21.165501010180094	27.040815051669526	28.20849828406814	23.58518565408224
44	20.351693151792436	28.92699356133391	29.00431530279166	21.716997984081992
45	19.157106950821984	32.552314335506395	26.101210133840187	22.189368579831438
46	20.74935178838108	31.138006676045226	27.065830092622285	21.04681144295141
47	20.28078481151125	28.415281885639892	28.932435235059483	22.37149806778937
48	21.386138668838896	26.525160938888614	30.78635239335083	21.30234799892166
49	21.205369599312352	26.19494018739903	30.79817970461151	21.80151050867711
50	19.681756483434963	30.550250386400478	28.5727017327011	21.19529139746346
51	18.86167404207802	30.85168024169916	27.61885406680432	22.667791649418504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2133.0
1	3807.5
2	5482.0
3	24954.5
4	44427.0
5	32243.5
6	20060.0
7	20447.0
8	20834.0
9	21906.0
10	22978.0
11	22893.0
12	22808.0
13	23138.5
14	23469.0
15	22149.0
16	20829.0
17	20604.0
18	20379.0
19	19497.0
20	18615.0
21	19737.0
22	20859.0
23	21660.5
24	22462.0
25	23514.5
26	30997.5
27	37428.0
28	44905.0
29	52382.0
30	59557.5
31	66733.0
32	76719.0
33	86705.0
34	101558.0
35	116411.0
36	126416.5
37	136422.0
38	152041.5
39	167661.0
40	193097.0
41	218533.0
42	253021.0
43	287509.0
44	288519.5
45	289530.0
46	287571.0
47	285612.0
48	271337.5
49	257063.0
50	249312.0
51	241561.0
52	225436.5
53	209312.0
54	192083.5
55	174855.0
56	167094.5
57	159334.0
58	146459.0
59	133584.0
60	121791.0
61	109998.0
62	99329.5
63	88661.0
64	77344.5
65	66028.0
66	54687.0
67	43346.0
68	36947.0
69	30548.0
70	26221.5
71	21895.0
72	17410.0
73	12925.0
74	10350.5
75	6412.0
76	5048.0
77	3747.5
78	2447.0
79	1830.5
80	1214.0
81	1028.5
82	843.0
83	592.5
84	342.0
85	215.5
86	89.0
87	94.0
88	99.0
89	56.0
90	13.0
91	9.5
92	6.0
93	9.0
94	12.0
95	7.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3601833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.28008336304713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14152846173326	14.649938211224981
2	6.604088349628988	2.414465711362925
3	2.3619020451213455	1.2952729884050909
4	1.31954197324778	0.9648534908803649
5	0.9352337822522887	0.8548075751754853
6	0.6881403895281809	0.7547558211632923
7	0.510863571204435	0.6537040068132725
8	0.4296832132111185	0.628371596576096
9	0.3441159205657261	0.5661420943043859
>10	3.811502245893122	16.79438201380051
>50	1.5078083605548973	19.938959241960408
>100	1.341742437275567	39.06824546102379
>500	0.0026174898527258805	0.30740889473881666
>1k	9.23819948020899E-4	0.24087212242948114
>5k	1.539699913368165E-4	0.16631378493056836
>10k+	1.539699913368165E-4	0.7015069852106085
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24924	0.6919809996743325	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5909	0.1640553573694283	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7763641457002588E-5	0.0	0.0	0.024737404538189307	0.0
2	2.7763641457002588E-5	0.0	0.0	0.0804035056594795	0.0
3	5.5527282914005176E-5	0.0	0.0	0.1211882949598163	0.0
4	5.5527282914005176E-5	0.0	0.0	0.19440101748193211	0.0
5	5.5527282914005176E-5	0.0	0.0	0.354458410481552	0.0
6	8.329092437100777E-5	0.0	0.0	0.6015825830903321	0.0
7	8.329092437100777E-5	0.0	0.0	0.7569756843251756	0.0
8	8.329092437100777E-5	0.0	0.0	1.0813383074673368	0.0
9	1.1105456582801035E-4	0.0	0.0	1.2490029382261754	0.0
10	1.1105456582801035E-4	0.0	0.0	1.4770534891539946	0.0
11	1.1105456582801035E-4	0.0	0.0	1.6692611789608236	0.0
12	1.3881820728501294E-4	0.0	0.0	1.8229329344253329	0.0
13	1.6658184874201554E-4	0.0	0.0	1.8972284389642717	0.0
14	1.6658184874201554E-4	0.0	0.0	1.9359031915138765	0.0
15	1.943454901990181E-4	0.0	0.0	1.9777707628310364	0.0
16	1.943454901990181E-4	0.0	0.0	2.0560364680983265	0.0
17	2.221091316560207E-4	0.0	0.0	2.145629739080074	0.0
18	2.221091316560207E-4	0.0	0.0	2.2662349975692933	0.0
19	2.498727731130233E-4	0.0	0.0	2.3332009007635834	0.0
20	2.498727731130233E-4	0.0	0.0	2.404192531969139	0.0
21	2.776364145700259E-4	0.0	0.0	2.49653440345513	0.0
22	3.0540005602702845E-4	0.0	0.0	2.6013143863138573	0.0
23	3.0540005602702845E-4	0.0	0.0	2.722530444915131	0.0
24	3.0540005602702845E-4	0.0	0.0	2.817870789678478	0.0
25	3.0540005602702845E-4	0.0	0.0	2.9031329325929325	0.0
26	3.0540005602702845E-4	0.0	0.0	2.9881174390928176	0.0
27	3.0540005602702845E-4	0.0	0.0	3.0730186546683314	0.0
28	3.0540005602702845E-4	0.0	0.0	3.1572535428488773	0.0
29	3.0540005602702845E-4	5.5527282914005176E-5	0.0	3.2578967431305115	0.0
30	3.0540005602702845E-4	5.5527282914005176E-5	0.0	3.388996658090478	0.0
31	3.3316369748403107E-4	5.5527282914005176E-5	0.0	3.4988851509772942	0.0
32	3.3316369748403107E-4	5.5527282914005176E-5	0.0	3.6109669715392134	0.0
33	3.3316369748403107E-4	5.5527282914005176E-5	0.0	3.7218271918770247	0.0
34	3.3316369748403107E-4	5.5527282914005176E-5	0.0	3.841183086500679	0.0
35	3.3316369748403107E-4	5.5527282914005176E-5	0.0	3.982028039612053	0.0
36	3.3316369748403107E-4	5.5527282914005176E-5	0.0	4.105076498549488	0.0
37	3.3316369748403107E-4	5.5527282914005176E-5	0.0	4.231789758159248	0.0
38	3.3316369748403107E-4	5.5527282914005176E-5	0.0	4.376216221018576	0.0
39	3.3316369748403107E-4	5.5527282914005176E-5	0.0	4.584915513850865	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	20	7.0352934E-4	45.0	25
CGTTTTT	19175	0.0	42.195568	1
CGCATTA	55	6.184564E-11	40.909092	45
GTCCGAT	40	3.4609548E-7	39.375	38
GCGATCG	160	0.0	37.96875	9
AATACGG	210	0.0	37.5	2
ACGGGTA	475	0.0	37.42105	5
AGGGCGA	3825	0.0	37.411766	6
TAGTACG	115	0.0	37.173912	1
ATACTAT	2655	0.0	37.0339	45
ACGTAGG	295	0.0	36.61017	2
GGGCGAT	7020	0.0	36.05769	7
CGACGTC	100	0.0	36.0	18
ATCGTAC	50	4.8839865E-8	36.0	28
CGTTAGG	400	0.0	36.0	2
CGAATCG	25	0.0021077043	36.0	22
TCGTACT	50	4.8839865E-8	36.0	29
CGGTAGT	1565	0.0	35.79872	12
GCTACGA	390	0.0	35.76923	10
TAGGGCG	1695	0.0	35.44248	5
>>END_MODULE