FastQCFastQC Report
Thu 26 May 2016
SRR1545398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545398_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1948337
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79220.40660316978017663No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74910.38448174006858155No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29180.14976875150448818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG600.045.0000041
ATCGTAG302.1662818E-645.0000041
TAATGCG1150.045.0000041
TCGCACC207.034495E-445.044
GGTCGAA253.8918075E-545.04
CGCATCG207.034495E-445.021
TATATCG253.8918075E-545.01
TGTATCG207.034495E-445.02
TAACGCG1000.045.01
ATCGTAC253.8918075E-545.010
CTAACGG1850.045.02
CGTAGTC253.8918075E-545.040
CTACGAT253.8918075E-545.023
TATTACG850.045.01
CGGTCCA253.8918075E-545.024
GCTAACG1350.045.01
CAATACG253.8918075E-545.01
GCGTAGC207.034495E-445.039
GCTACGA2300.043.0434810
TACGTAG900.042.51
Produced by FastQC (version 0.11.5)