##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545398_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1948337
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413137973564123	31.0	31.0	33.0	30.0	34.0
2	31.76649727434217	31.0	31.0	34.0	30.0	34.0
3	31.852244760531676	31.0	31.0	34.0	30.0	34.0
4	35.551330698949926	37.0	35.0	37.0	33.0	37.0
5	35.41714857337309	37.0	35.0	37.0	33.0	37.0
6	35.48936503284596	37.0	35.0	37.0	33.0	37.0
7	35.909319588962276	37.0	35.0	37.0	35.0	37.0
8	35.9519667285485	37.0	35.0	37.0	35.0	37.0
9	37.67189095110343	39.0	37.0	39.0	35.0	39.0
10	37.0854220804717	39.0	37.0	39.0	33.0	39.0
11	36.69107141115731	39.0	35.0	39.0	32.0	39.0
12	35.6703352654084	37.0	35.0	39.0	31.0	39.0
13	35.21527333310408	37.0	35.0	39.0	30.0	39.0
14	36.14968868322061	38.0	35.0	40.0	31.0	41.0
15	36.44973687816841	38.0	35.0	40.0	31.0	41.0
16	36.67260284026839	38.0	35.0	40.0	32.0	41.0
17	36.646138219414816	38.0	35.0	40.0	32.0	41.0
18	36.60007329327524	38.0	35.0	40.0	32.0	41.0
19	36.53314493334572	37.0	35.0	40.0	31.0	41.0
20	36.364435926638976	37.0	35.0	40.0	31.0	41.0
21	36.15824829072178	37.0	35.0	40.0	31.0	41.0
22	36.088418482018255	37.0	35.0	40.0	31.0	41.0
23	36.05423291761127	36.0	35.0	40.0	31.0	41.0
24	35.971252919797756	36.0	34.0	40.0	31.0	41.0
25	35.86992137397175	36.0	34.0	40.0	31.0	41.0
26	35.75725657316983	36.0	34.0	40.0	30.0	41.0
27	35.72823438655633	36.0	34.0	40.0	30.0	41.0
28	35.76292037773753	36.0	34.0	40.0	30.0	41.0
29	35.793386359751935	36.0	35.0	40.0	30.0	41.0
30	35.73014473368827	36.0	35.0	40.0	30.0	41.0
31	35.52875708873773	36.0	34.0	40.0	30.0	41.0
32	35.30817923182694	36.0	34.0	40.0	29.0	41.0
33	35.135020276266374	36.0	34.0	40.0	29.0	41.0
34	34.97119235532662	35.0	34.0	40.0	28.0	41.0
35	34.798511243178154	35.0	34.0	40.0	27.0	41.0
36	34.59864284258832	35.0	33.0	40.0	26.0	41.0
37	34.513062165323554	35.0	33.0	40.0	26.0	41.0
38	34.527934849053324	35.0	33.0	40.0	26.0	41.0
39	34.49303534244846	35.0	33.0	40.0	26.0	41.0
40	34.29556488430903	35.0	33.0	40.0	24.0	41.0
41	34.31429932296107	35.0	33.0	40.0	24.0	41.0
42	34.237379365068776	35.0	33.0	40.0	25.0	41.0
43	34.15610646412813	35.0	33.0	40.0	24.0	41.0
44	34.06324213932189	35.0	33.0	40.0	24.0	41.0
45	33.989069139476385	35.0	33.0	40.0	24.0	41.0
46	33.990024313042355	35.0	33.0	39.0	24.0	41.0
47	33.971586024389005	35.0	33.0	39.0	24.0	41.0
48	33.97663494559719	35.0	33.0	39.0	24.0	41.0
49	33.92848105846165	35.0	33.0	39.0	24.0	41.0
50	33.77483926035383	35.0	33.0	39.0	24.0	41.0
51	33.60291315106165	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	10.0
14	14.0
15	38.0
16	129.0
17	325.0
18	926.0
19	1739.0
20	3130.0
21	5114.0
22	7872.0
23	11427.0
24	16897.0
25	23687.0
26	31679.0
27	36506.0
28	40535.0
29	46449.0
30	55912.0
31	67876.0
32	85680.0
33	113717.0
34	203259.0
35	328821.0
36	132245.0
37	155693.0
38	219864.0
39	358609.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.45058426750608	25.009225816683667	30.198471824946093	14.341718090864159
2	29.357806170082483	26.55136149444372	30.758539205486525	13.332293129987265
3	26.90961573896097	25.736204773609494	33.15191365764752	14.202265829782016
4	25.765152537779656	27.634336359674943	32.011864477243925	14.588646625301473
5	22.207708420052587	32.757628685386564	30.56606736924875	14.4685955253121
6	21.690652079183426	40.16019815873742	26.936407818565268	11.21274194351388
7	86.56849405416004	4.950734908796579	6.8503036179059364	1.630467419137449
8	88.55079998994015	3.369437628090007	6.521407744142825	1.5583546378270288
9	84.28285250446919	4.696826062431705	8.193141124969653	2.8271803081294458
10	55.41284695614773	20.784032741769007	13.894310891801572	9.908809410281691
11	48.77472429051032	19.061486796175405	19.157158130241328	13.006630783072948
12	43.61765957326684	20.05181855089751	23.799989426880465	12.530532448955187
13	20.473049580231756	44.25035299334766	21.882918612129217	13.393678814291368
14	14.62005802897548	43.714460075438694	26.952986059393215	14.71249583619261
15	13.037631580162982	21.5635180156205	51.0392709269495	14.359579477267022
16	14.380417761403699	16.483903965279108	48.58533200365234	20.55034626966485
17	14.925549327452078	17.512268154841795	28.755446311392742	38.80673620631339
18	20.425213913198796	21.684492980423816	36.932830408702394	20.95746269767499
19	28.890843832458142	23.873641982880788	25.779010509988776	21.456503674672298
20	31.967878246935722	20.755906190766794	26.591139007266197	20.68507655503129
21	20.907009413669194	27.987560673538507	28.736661060175933	22.368768852616363
22	22.72979469157543	24.09290589872286	24.12329078593693	29.054008623764783
23	19.31272669974445	30.464955497945173	23.886062832045994	26.336254970264385
24	18.616645888262656	21.96596379373794	42.5476188154308	16.8697715025686
25	16.208694902370585	25.884331098778084	37.15609773873821	20.750876260113113
26	16.148079105411437	34.77242386712361	27.798168386680537	21.281328640784423
27	16.219319347730913	35.92473991922342	27.994130378882094	19.861810354163577
28	13.99968280641388	28.917481934593454	39.039139532842626	18.043695726150045
29	14.381649581155623	22.725175367505724	38.79195436929032	24.101220682048332
30	17.266520114333403	32.0626257161877	32.006167310891286	18.664686858587608
31	28.94755886686954	27.60071794561208	25.61861731312396	17.83310587439442
32	29.811937051957642	25.341149914003587	26.72571531516365	18.121197718875123
33	29.54411890756065	27.938852467514604	23.689690233260468	18.827338391664274
34	19.892195241377646	25.98143955588792	27.422925294751373	26.70343990798306
35	19.74981740838469	25.901011991252027	31.89540618486432	22.453764415498963
36	31.678554582703093	24.538568019803556	25.01584684785024	18.767030549643106
37	19.868944643560123	33.548354314474345	29.721706255129376	16.86099478683616
38	20.225094529334502	31.963566877803995	26.03794928700733	21.77338930585417
39	20.78023463086725	32.94825279199646	27.2523695849332	19.019142992203093
40	24.84919190057983	25.065581570334082	26.952883407747226	23.132343121338863
41	17.284278849090278	24.085412328565337	27.883112623740143	30.747196198604243
42	21.301602340868136	24.004163550761497	25.741337355909167	28.9528967524612
43	21.850121411234298	25.36409255688313	26.979316206590543	25.806469825292034
44	18.93034931841873	29.76020062237693	29.878147363623437	21.431302695580897
45	16.330901686925824	39.46750485157342	22.530907127463063	21.670686334037693
46	20.990516527684893	33.86282763197537	26.123201479004916	19.02345436133482
47	20.77284371235572	27.993822423944113	26.805629621569576	24.427704242130595
48	22.18276407007617	23.980143065599023	32.24082897363238	21.59626389069242
49	21.292209715259734	25.158481309958187	32.55643145923934	20.992877515542745
50	19.19616575571885	35.29173854420462	26.303457769369466	19.20863793070706
51	18.25757043057746	33.96091127972215	25.009585097444642	22.771933192255755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1056.0
1	1834.5
2	2613.0
3	7641.0
4	12669.0
5	9592.0
6	6515.0
7	7243.5
8	7972.0
9	8790.5
10	9609.0
11	10027.5
12	10446.0
13	10537.0
14	10628.0
15	10064.5
16	9501.0
17	9001.5
18	8502.0
19	8384.5
20	8267.0
21	7839.0
22	7411.0
23	7305.5
24	7200.0
25	8244.5
26	11107.5
27	12926.0
28	14818.5
29	16711.0
30	24854.0
31	32997.0
32	36916.5
33	40836.0
34	49484.0
35	58132.0
36	61020.5
37	63909.0
38	76170.0
39	88431.0
40	118725.0
41	149019.0
42	176776.0
43	204533.0
44	202997.0
45	201461.0
46	186936.5
47	172412.0
48	162655.0
49	152898.0
50	150045.0
51	147192.0
52	130451.5
53	113711.0
54	100207.0
55	86703.0
56	76112.0
57	65521.0
58	59473.5
59	53426.0
60	50168.0
61	46910.0
62	42470.5
63	38031.0
64	33590.5
65	29150.0
66	25431.5
67	21713.0
68	18665.0
69	15617.0
70	12580.0
71	9543.0
72	7576.0
73	5609.0
74	4774.0
75	3209.0
76	2479.0
77	2040.5
78	1602.0
79	1173.0
80	744.0
81	526.5
82	309.0
83	214.0
84	119.0
85	76.0
86	33.0
87	31.5
88	30.0
89	19.0
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1948337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.877115779658602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51963060613544	12.784194976689545
2	7.197398921434591	2.2854787197601385
3	2.5355183869662423	1.2077015697394875
4	1.2603227318001164	0.8004115973010426
5	0.7365714403690207	0.5847315019364422
6	0.5052973526392638	0.48135987426051435
7	0.3523718355937391	0.3916253901848835
8	0.293551950516186	0.3728606644552078
9	0.22898968420457283	0.3272126155617117
>10	2.9766904561653713	11.83970183530957
>50	1.587938413210351	18.41376993491183
>100	1.797905486639738	48.87161062062301
>500	0.006510611937858511	0.6317441631608617
>1k	6.510611937858512E-4	0.21097763838850014
>5k	6.510611937858512E-4	0.7966188977172838
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7922	0.40660316978017663	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7491	0.38448174006858155	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2918	0.14976875150448818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021864800596611368	0.0
2	0.0	0.0	0.0	0.07544895980520824	0.0
3	0.0	0.0	0.0	0.10937532880605358	0.0
4	0.0	0.0	0.0	0.17342995590598545	0.0
5	0.0	0.0	0.0	0.2884511252416805	0.0
6	0.0	0.0	0.0	0.43724468610923056	0.0
7	0.0	0.0	0.0	0.5258330566016043	0.0
8	0.0	0.0	0.0	0.7561833502109748	0.0
9	0.0	0.0	0.0	0.8764397534923373	0.0
10	0.0	0.0	0.0	1.082461607001253	0.0
11	0.0	0.0	0.0	1.2919736164739468	0.0
12	0.0	0.0	0.0	1.4750528271033194	0.0
13	0.0	0.0	0.0	1.5544538752792767	0.0
14	0.0	0.0	0.0	1.5834016394494381	0.0
15	0.0	0.0	0.0	1.6234357813868956	0.0
16	0.0	0.0	0.0	1.7114595678263052	0.0
17	0.0	0.0	0.0	1.8126740907758772	0.0
18	0.0	0.0	0.0	1.9485848700712454	0.0
19	5.13258229967403E-5	0.0	0.0	2.0123828680561937	0.0
20	1.026516459934806E-4	0.0	0.0	2.078644505544985	0.0
21	1.026516459934806E-4	0.0	0.0	2.1749317494868703	0.0
22	1.026516459934806E-4	0.0	0.0	2.276094946613445	0.0
23	1.026516459934806E-4	0.0	0.0	2.4007140448495305	0.0
24	1.026516459934806E-4	0.0	0.0	2.4865308209000805	0.0
25	1.026516459934806E-4	0.0	0.0	2.5557180302996865	0.0
26	1.026516459934806E-4	0.0	0.0	2.6273688792031358	0.0
27	1.026516459934806E-4	0.0	0.0	2.6986091215226113	0.0
28	1.026516459934806E-4	0.0	0.0	2.7679503083912076	0.0
29	1.026516459934806E-4	0.0	0.0	2.8432452907274257	0.0
30	1.026516459934806E-4	0.0	0.0	2.9575478985411663	0.0
31	1.026516459934806E-4	0.0	0.0	3.058762421490738	0.0
32	1.026516459934806E-4	0.0	0.0	3.1451951074172486	0.0
33	1.026516459934806E-4	0.0	0.0	3.2287535472559417	0.0
34	1.539774689902209E-4	0.0	0.0	3.3246815104368497	0.0
35	2.053032919869612E-4	0.0	0.0	3.4577180436444004	0.0
36	2.053032919869612E-4	0.0	0.0	3.5565202529131255	0.0
37	2.053032919869612E-4	0.0	0.0	3.6580940566236744	0.0
38	2.053032919869612E-4	0.0	0.0	3.7558697494324647	0.0
39	2.053032919869612E-4	0.0	0.0	3.8715068286441205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	60	0.0	45.000004	1
ATCGTAG	30	2.1662818E-6	45.000004	1
TAATGCG	115	0.0	45.000004	1
TCGCACC	20	7.034495E-4	45.0	44
GGTCGAA	25	3.8918075E-5	45.0	4
CGCATCG	20	7.034495E-4	45.0	21
TATATCG	25	3.8918075E-5	45.0	1
TGTATCG	20	7.034495E-4	45.0	2
TAACGCG	100	0.0	45.0	1
ATCGTAC	25	3.8918075E-5	45.0	10
CTAACGG	185	0.0	45.0	2
CGTAGTC	25	3.8918075E-5	45.0	40
CTACGAT	25	3.8918075E-5	45.0	23
TATTACG	85	0.0	45.0	1
CGGTCCA	25	3.8918075E-5	45.0	24
GCTAACG	135	0.0	45.0	1
CAATACG	25	3.8918075E-5	45.0	1
GCGTAGC	20	7.034495E-4	45.0	39
GCTACGA	230	0.0	43.04348	10
TACGTAG	90	0.0	42.5	1
>>END_MODULE