FastQCFastQC Report
Thu 26 May 2016
SRR1545397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545397_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5401818
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298820.5531841317126938No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC130960.24243689809615945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG502.1827873E-1145.01
ACGTTAG2400.042.1874961
AAATGCG2050.041.7073141
CGTAAGG5500.041.3181842
ACGGGTA3200.040.781255
TATACGT451.9304935E-840.044
CGTTTTT200500.039.8940161
CGTTAGG7450.039.2617452
TAAGGGA42900.038.6014024
TATCGAT356.2525833E-638.57142638
TACGGGA13400.038.2835854
TATAGCG3200.037.968751
ATACTAT31150.037.77688645
GTTGATC20450.037.73838816
AATGCGG5850.037.692312
CGTATCA301.1404815E-437.49999615
GGGCGAT101950.037.4080437
TAGCATA20500.037.09755730
CGAACTA552.75395E-936.8181833
CATATGC20900.036.38755833
Produced by FastQC (version 0.11.5)