##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545397_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5401818
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284669716750916	31.0	31.0	33.0	30.0	34.0
2	31.638724222104486	31.0	31.0	34.0	30.0	34.0
3	31.724089926761692	31.0	31.0	34.0	30.0	34.0
4	35.43935319553528	37.0	35.0	37.0	33.0	37.0
5	35.2946106292363	37.0	35.0	37.0	33.0	37.0
6	35.36097051029857	37.0	35.0	37.0	33.0	37.0
7	35.82222799805547	37.0	35.0	37.0	35.0	37.0
8	35.849248345649556	37.0	35.0	37.0	35.0	37.0
9	37.5119654160877	39.0	37.0	39.0	35.0	39.0
10	36.88841682559464	39.0	37.0	39.0	32.0	39.0
11	36.524503417182885	39.0	35.0	39.0	32.0	39.0
12	35.832421788368286	37.0	35.0	39.0	31.0	39.0
13	35.53423532595878	37.0	35.0	39.0	30.0	39.0
14	36.53137332653562	38.0	35.0	41.0	31.0	41.0
15	36.72102503268344	38.0	35.0	41.0	31.0	41.0
16	36.85194965842981	38.0	35.0	41.0	32.0	41.0
17	36.78641672118535	38.0	35.0	41.0	31.0	41.0
18	36.67884923186971	38.0	35.0	40.0	31.0	41.0
19	36.63116676644789	38.0	35.0	40.0	31.0	41.0
20	36.489000554998334	38.0	35.0	40.0	31.0	41.0
21	36.29773550312136	38.0	35.0	40.0	31.0	41.0
22	36.217382185034744	38.0	35.0	40.0	30.0	41.0
23	36.149532250068404	38.0	35.0	40.0	30.0	41.0
24	36.086836320660936	37.0	35.0	40.0	30.0	41.0
25	35.964642459260936	37.0	34.0	40.0	30.0	41.0
26	35.86776933247288	37.0	34.0	40.0	30.0	41.0
27	35.8181876916253	37.0	34.0	40.0	30.0	41.0
28	35.785251928147154	37.0	34.0	40.0	30.0	41.0
29	35.77293144641304	37.0	34.0	40.0	30.0	41.0
30	35.67310079680582	37.0	34.0	40.0	29.0	41.0
31	35.515116762541794	37.0	34.0	40.0	29.0	41.0
32	35.32632902478388	37.0	34.0	40.0	29.0	41.0
33	35.14028702929273	37.0	34.0	40.0	27.0	41.0
34	34.93233833498278	37.0	34.0	40.0	26.0	41.0
35	34.77152358705902	36.0	34.0	40.0	25.0	41.0
36	34.62534354174835	36.0	33.0	40.0	25.0	41.0
37	34.55063350894088	36.0	33.0	40.0	24.0	41.0
38	34.51733120219896	36.0	33.0	40.0	25.0	41.0
39	34.43382764839541	36.0	33.0	40.0	24.0	41.0
40	34.2560410217449	36.0	33.0	40.0	23.0	41.0
41	34.210301235621046	36.0	33.0	40.0	23.0	41.0
42	34.116820485251445	36.0	33.0	40.0	23.0	41.0
43	34.03445599240848	35.0	33.0	40.0	23.0	41.0
44	33.93651100425819	35.0	33.0	40.0	23.0	41.0
45	33.89470711527119	35.0	33.0	40.0	23.0	41.0
46	33.857723085079876	35.0	33.0	40.0	23.0	41.0
47	33.800477913176636	35.0	33.0	40.0	23.0	41.0
48	33.74815589862524	35.0	33.0	39.0	23.0	41.0
49	33.67429150704448	35.0	33.0	39.0	23.0	41.0
50	33.54022256951271	35.0	33.0	39.0	23.0	41.0
51	33.35479333068978	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	9.0
13	15.0
14	51.0
15	181.0
16	515.0
17	1469.0
18	3295.0
19	6463.0
20	11118.0
21	17526.0
22	25974.0
23	36959.0
24	51270.0
25	70944.0
26	92763.0
27	110195.0
28	122899.0
29	139291.0
30	162668.0
31	196129.0
32	239266.0
33	303853.0
34	492171.0
35	720280.0
36	409341.0
37	492527.0
38	667507.0
39	1026642.0
40	496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.763435939529987	24.170047935713495	29.303560393926638	14.76295573082988
2	31.668467912099224	25.929140892936413	29.01552773529208	13.386863459672279
3	28.25741259701826	25.476885744762228	32.32134070418515	13.944360954034362
4	26.17857543515905	27.45220220303609	31.137257863926553	15.231964497878305
5	23.344918321942725	31.72139453791298	30.242651640614326	14.691035499529972
6	21.912974483775645	39.999570514963665	27.14880434698096	10.938650654279726
7	85.35452323643634	4.907273810409754	7.6951130156551	2.04308993749882
8	86.56328295399808	3.8476305569717457	7.44986225007951	2.1392242389506646
9	81.37404851477781	5.633251620102714	9.405333537708971	3.5873663274105128
10	49.07753278618421	22.554443707655462	15.50313246392233	12.864891042238003
11	40.74955875966202	21.96175435751445	21.584992311847603	15.703694570975921
12	35.85992715785685	21.217578970635444	26.97725099216597	15.945242879341734
13	22.52624949600301	33.83571975212789	26.689181308959313	16.948849442909776
14	17.521619573262186	35.607974944731566	29.691281712934426	17.17912376907182
15	17.28181141978497	22.768186562375853	43.848941226824	16.101060791015172
16	19.32854827763542	18.94867616791236	41.64895966506091	20.073815889391312
17	19.987974418982645	19.728580266865713	29.402527075143958	30.88091823900768
18	22.152060658096957	22.984835846005918	33.66727646136912	21.195827034528005
19	29.03472497592477	22.80945044797881	26.866621570737852	21.289203005358566
20	30.267754300496613	22.078104075331677	26.503003248165708	21.151138376006003
21	25.131650122236625	26.228262410914247	28.077491688909177	20.562595777939947
22	24.526057708719545	23.363726804568387	26.35303521888372	25.75718026782835
23	21.87365438820782	27.234553255959383	25.92395745284273	24.96783490299007
24	21.97484254375101	22.73134711313858	35.82342092976846	19.47038941334195
25	20.720153844501983	23.29839694710188	33.727034120734906	22.25441508766123
26	19.04225577388946	30.23617234049722	28.539595373261374	22.18197651235195
27	19.861924263275807	31.214028314171266	28.40517766426044	20.518869758292485
28	18.642927251529024	26.989580174674526	34.56223441811627	19.805258155680182
29	19.54101378461844	23.83714149569645	34.44719907260852	22.174645647076595
30	20.380397858646848	28.532745827423284	30.694258858776802	20.392597455153062
31	27.61531395541279	25.696996825883435	26.67309413238284	20.014595086320938
32	29.27088620905036	24.696759498376288	27.683735364649458	18.348618927923894
33	28.357230843393832	25.62702038461866	25.805145600981007	20.210603171006504
34	22.206560828224866	26.437821488987595	28.166110002225174	23.18950768056236
35	22.368173085431607	24.795485519874973	30.640887938097876	22.195453456595537
36	28.63171250864061	25.786207532352996	26.210490616307325	19.371589342699068
37	22.716000428004055	30.718454416642693	27.907808074985123	18.65773708036813
38	22.33242586107122	30.221862343381435	25.28458011728644	22.16113167826091
39	22.687158286339894	29.79115549616814	26.63460708968721	20.88707912780475
40	24.66554778409787	25.635869257350024	26.974585222974934	22.72399773557717
41	19.630261515660095	25.171747733818506	27.63166030399395	27.56633044652745
42	22.196989976337598	25.39469119470519	25.80969962334903	26.598619205608188
43	22.95399437744848	25.007525244278874	26.75402984698855	25.284450531284097
44	20.965774855798546	27.811821871821675	28.722015439987057	22.500387832392725
45	19.846299893850553	32.788368656626346	25.137129758907093	22.22820169061601
46	21.996631504430546	30.642942801849305	26.752863572967474	20.60756212075268
47	22.41349116167927	27.199139252747873	27.012979704240315	23.374389881332544
48	23.344714686796188	24.178804247014615	30.372311692100695	22.1041693740885
49	22.51269850261523	24.310482137680314	31.072409325897315	22.104410033807138
50	20.813122545039466	30.318366890554255	27.288183348642992	21.580327215763287
51	19.84898417532764	30.71051264592772	26.513925496934547	22.926577681810087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3446.0
1	7525.0
2	11604.0
3	32134.5
4	52665.0
5	38031.0
6	23397.0
7	23831.5
8	24266.0
9	25338.5
10	26411.0
11	27248.5
12	28086.0
13	27465.0
14	26844.0
15	26596.5
16	26349.0
17	24865.0
18	23381.0
19	22952.0
20	22523.0
21	23106.0
22	23689.0
23	25402.0
24	27115.0
25	30680.0
26	41134.0
27	48023.0
28	52864.5
29	57706.0
30	67711.0
31	77716.0
32	91374.5
33	105033.0
34	124579.5
35	144126.0
36	146863.0
37	149600.0
38	171307.5
39	193015.0
40	253778.5
41	314542.0
42	370392.0
43	426242.0
44	443830.5
45	461419.0
46	442217.5
47	423016.0
48	409846.0
49	396676.0
50	386791.5
51	376907.0
52	350320.5
53	323734.0
54	298534.0
55	273334.0
56	262385.0
57	251436.0
58	240897.0
59	230358.0
60	219711.0
61	209064.0
62	190233.0
63	171402.0
64	152843.0
65	134284.0
66	114976.0
67	95668.0
68	82438.0
69	69208.0
70	57131.0
71	45054.0
72	37128.0
73	29202.0
74	23280.0
75	14209.0
76	11060.0
77	8959.5
78	6859.0
79	5035.0
80	3211.0
81	2509.0
82	1807.0
83	1121.5
84	436.0
85	307.0
86	178.0
87	115.0
88	52.0
89	45.5
90	39.0
91	28.5
92	18.0
93	13.5
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5401818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.612436632414008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.03835680378762	11.84167853590303
2	7.474956403921534	2.1845465356472142
3	2.3864336135054582	1.0461482986443387
4	1.2575439583777113	0.7350312561707752
5	0.8601654565766118	0.6284556613808602
6	0.5978314030923195	0.5241464096732205
7	0.42301219252162553	0.4326867200572538
8	0.32981415239570916	0.3855510721884509
9	0.2652510926731403	0.34883683050283537
>10	2.3947272593857005	7.9102983387774
>50	1.0186746526147508	10.979811161111218
>100	1.9121068721969812	58.01687723843955
>500	0.036898218124063666	3.281990216248329
>1k	0.003971683200854075	0.879338538213795
>5k	0.0	0.0
>10k+	2.562376258615532E-4	0.8046031870417818
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29882	0.5531841317126938	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	13096	0.24243689809615945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014402558546030245	0.0
2	0.0	0.0	0.0	0.06895826553208569	0.0
3	0.0	0.0	0.0	0.12079266646895545	0.0
4	0.0	0.0	0.0	0.2080595829033855	0.0
5	0.0	0.0	0.0	0.36061933223222253	0.0
6	0.0	0.0	0.0	0.5696600662961988	0.0
7	0.0	0.0	0.0	0.6956546851448902	0.0
8	0.0	0.0	0.0	0.9628425096884049	0.0
9	0.0	0.0	0.0	1.0946129617843474	0.0
10	0.0	0.0	0.0	1.2927869839376298	0.0
11	0.0	0.0	0.0	1.477539598705473	0.0
12	0.0	0.0	0.0	1.6374302133096672	0.0
13	3.7024572097764124E-5	0.0	0.0	1.709813251760796	0.0
14	3.7024572097764124E-5	0.0	0.0	1.7401548885949139	0.0
15	9.256143024441031E-5	0.0	0.0	1.776216821818136	0.0
16	9.256143024441031E-5	0.0	0.0	1.857059974993604	0.0
17	9.256143024441031E-5	0.0	0.0	1.9475850537726374	0.0
18	9.256143024441031E-5	0.0	0.0	2.071839517732734	0.0
19	1.1107371629329237E-4	0.0	0.0	2.138057964929585	0.0
20	1.1107371629329237E-4	0.0	0.0	2.210237368234176	0.0
21	1.1107371629329237E-4	0.0	0.0	2.3024470650436575	0.0
22	1.1107371629329237E-4	0.0	0.0	2.395600888441632	0.0
23	1.1107371629329237E-4	0.0	0.0	2.4989920060246384	0.0
24	1.1107371629329237E-4	0.0	0.0	2.5845002552844245	0.0
25	1.1107371629329237E-4	0.0	0.0	2.6540138893979766	1.8512286048882062E-5
26	1.1107371629329237E-4	0.0	0.0	2.7278408861609185	1.8512286048882062E-5
27	1.1107371629329237E-4	0.0	0.0	2.796058660251049	1.8512286048882062E-5
28	1.480982883910565E-4	0.0	0.0	2.8681455021253957	1.8512286048882062E-5
29	1.6661057443993855E-4	0.0	0.0	2.947155938982024	1.8512286048882062E-5
30	1.6661057443993855E-4	0.0	0.0	3.046233693915641	1.8512286048882062E-5
31	1.8512286048882063E-4	0.0	0.0	3.1404612299044508	1.8512286048882062E-5
32	1.8512286048882063E-4	0.0	0.0	3.2272838514737074	1.8512286048882062E-5
33	2.0363514653770268E-4	0.0	0.0	3.3170684388107854	1.8512286048882062E-5
34	2.2214743258658473E-4	0.0	0.0	3.410851679934422	1.8512286048882062E-5
35	2.2214743258658473E-4	0.0	0.0	3.525905537728224	1.8512286048882062E-5
36	2.2214743258658473E-4	0.0	0.0	3.628667237585568	1.8512286048882062E-5
37	2.591720046843489E-4	0.0	0.0	3.7358163492364977	1.8512286048882062E-5
38	2.96196576782113E-4	0.0	0.0	3.835875255330705	1.8512286048882062E-5
39	3.332211488798771E-4	0.0	0.0	3.9411731383767465	1.8512286048882062E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	50	2.1827873E-11	45.0	1
ACGTTAG	240	0.0	42.187496	1
AAATGCG	205	0.0	41.707314	1
CGTAAGG	550	0.0	41.318184	2
ACGGGTA	320	0.0	40.78125	5
TATACGT	45	1.9304935E-8	40.0	44
CGTTTTT	20050	0.0	39.894016	1
CGTTAGG	745	0.0	39.261745	2
TAAGGGA	4290	0.0	38.601402	4
TATCGAT	35	6.2525833E-6	38.571426	38
TACGGGA	1340	0.0	38.283585	4
TATAGCG	320	0.0	37.96875	1
ATACTAT	3115	0.0	37.776886	45
GTTGATC	2045	0.0	37.738388	16
AATGCGG	585	0.0	37.69231	2
CGTATCA	30	1.1404815E-4	37.499996	15
GGGCGAT	10195	0.0	37.408043	7
TAGCATA	2050	0.0	37.097557	30
CGAACTA	55	2.75395E-9	36.81818	33
CATATGC	2090	0.0	36.387558	33
>>END_MODULE