FastQCFastQC Report
Thu 26 May 2016
SRR1545396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545396_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6580301
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406830.6182543929221475No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC213250.3240733212660029No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT81380.1236721542069276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC502.1827873E-1145.011
ATCGTAC850.042.3529429
CGTTTTT317000.041.585961
CGCGATA603.6379788E-1241.25000424
CGTAAGG11850.041.202532
CGACGGT4050.041.1111128
GCGTAAG7100.040.8802831
GCTACGA9750.040.84615310
TAATGCG1400.040.178571
AACGCGC451.9306754E-840.012
ACGGGTA8200.039.2378045
TCGATAG1950.039.230771
TAGGGCG28300.039.1166085
CGTTGAT13950.038.87096425
CGGTCTA4300.038.72093231
TCGTAAG3200.038.6718751
TGGGCGA43950.038.651886
CGTTATC700.038.57142624
CGGGATA5150.038.44666
ACCTACG659.094947E-1238.0769226
Produced by FastQC (version 0.11.5)