FastQCFastQC Report
Thu 26 May 2016
SRR1545395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545395_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4147715
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209170.5043017661531711No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC71190.1716366722400165No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT55470.13373628612380553No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG43480.1048288033290619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG750.045.01
CGTATTA502.1827873E-1145.042
AATACGG2600.042.4038432
TCGATAG1900.041.447371
TCGTTAA451.9303116E-840.024
CGTTTTT166650.039.8289831
CGACGGT4150.039.57831628
AGTCGAC403.4611367E-739.37530
TAGCATA15400.039.1558430
CGGTCTA3800.039.07894531
CCCGTAA356.252232E-638.5714322
ACGTAAC356.252232E-638.5714337
GCGATAT6500.038.4230739
AACGGGA10800.038.1254
GGCGATA21550.038.004648
TAACGGG10650.037.81693
TGGGCGA29200.037.6797946
GTTGATC16450.037.61398316
GGGCGAT95250.037.582687
TCGTATC301.1404353E-437.50000442
Produced by FastQC (version 0.11.5)