##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545395_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4147715
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290652564122656	31.0	31.0	33.0	30.0	34.0
2	31.636866322782545	31.0	31.0	34.0	30.0	34.0
3	31.727090940433467	31.0	31.0	34.0	30.0	34.0
4	35.446332257640655	37.0	35.0	37.0	33.0	37.0
5	35.31644170344394	37.0	35.0	37.0	33.0	37.0
6	35.376414483637376	37.0	35.0	37.0	33.0	37.0
7	35.827238612103294	37.0	35.0	37.0	35.0	37.0
8	35.844220733584635	37.0	35.0	37.0	35.0	37.0
9	37.494531085187866	39.0	37.0	39.0	35.0	39.0
10	36.88337916177944	39.0	37.0	39.0	32.0	39.0
11	36.53372423129362	39.0	35.0	39.0	32.0	39.0
12	35.85650581103089	37.0	35.0	39.0	31.0	39.0
13	35.5495961029145	37.0	35.0	39.0	30.0	39.0
14	36.571491532084536	38.0	35.0	41.0	31.0	41.0
15	36.75474640856471	38.0	35.0	41.0	31.0	41.0
16	36.894307106442945	38.0	35.0	41.0	32.0	41.0
17	36.83454528577783	38.0	35.0	41.0	32.0	41.0
18	36.743694781343464	38.0	35.0	40.0	31.0	41.0
19	36.68301317713488	38.0	35.0	40.0	31.0	41.0
20	36.52320398098712	38.0	35.0	40.0	31.0	41.0
21	36.343310473357015	38.0	35.0	40.0	31.0	41.0
22	36.27165535722681	38.0	35.0	40.0	30.0	41.0
23	36.21075363181897	38.0	35.0	40.0	30.0	41.0
24	36.12859369556491	37.0	35.0	40.0	30.0	41.0
25	36.00147671669823	37.0	34.0	40.0	30.0	41.0
26	35.89527607369359	37.0	34.0	40.0	30.0	41.0
27	35.85850594845596	37.0	34.0	40.0	30.0	41.0
28	35.85520605923985	37.0	34.0	40.0	30.0	41.0
29	35.834432452567256	37.0	34.0	40.0	30.0	41.0
30	35.73733079538975	37.0	34.0	40.0	30.0	41.0
31	35.5611340219856	37.0	34.0	40.0	29.0	41.0
32	35.3906808929736	37.0	34.0	40.0	29.0	41.0
33	35.18935365616972	37.0	34.0	40.0	27.0	41.0
34	34.99615041052724	37.0	34.0	40.0	27.0	41.0
35	34.832223766579915	37.0	34.0	40.0	26.0	41.0
36	34.676198340532075	36.0	33.0	40.0	25.0	41.0
37	34.60747761116663	36.0	33.0	40.0	25.0	41.0
38	34.576283809278124	36.0	33.0	40.0	25.0	41.0
39	34.496589085797844	36.0	33.0	40.0	24.0	41.0
40	34.33428309322121	36.0	33.0	40.0	23.0	41.0
41	34.280419459871275	36.0	33.0	40.0	23.0	41.0
42	34.186890613265376	36.0	33.0	40.0	23.0	41.0
43	34.11190788180962	35.0	33.0	40.0	23.0	41.0
44	34.05219741472112	35.0	33.0	40.0	23.0	41.0
45	34.00002941378566	35.0	33.0	40.0	23.0	41.0
46	33.95960233526171	35.0	33.0	40.0	23.0	41.0
47	33.88606425465588	35.0	33.0	40.0	23.0	41.0
48	33.84283949114151	35.0	33.0	40.0	23.0	41.0
49	33.776268137998876	35.0	33.0	39.0	24.0	41.0
50	33.65475930723302	35.0	33.0	39.0	23.0	41.0
51	33.493561394647415	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	2.0
13	12.0
14	52.0
15	167.0
16	432.0
17	1100.0
18	2607.0
19	5043.0
20	8573.0
21	13270.0
22	19907.0
23	28438.0
24	39803.0
25	54724.0
26	70294.0
27	81509.0
28	91278.0
29	103364.0
30	121598.0
31	147606.0
32	181528.0
33	230876.0
34	376133.0
35	559013.0
36	311025.0
37	370612.0
38	509080.0
39	819170.0
40	495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.598113660171926	23.304831696488307	28.478837142860584	14.61821750047918
2	31.826656363805135	25.80825828196971	29.355729600514984	13.009355753710174
3	28.891883844478222	24.92953349012649	32.108787609563336	14.069795055831946
4	26.092776384105466	27.7389357754812	30.93985483573486	15.228433004678479
5	23.581827584585728	31.981633260723076	29.56859379200355	14.867945362687648
6	21.938055049587543	40.14875660454009	27.31638986767413	10.596798478198236
7	84.9356814535232	5.084413948402916	7.848875826810666	2.1310287712632134
8	85.69513575547018	4.155396405008541	7.725723681593359	2.4237441579279193
9	80.64734438118337	6.097767083803974	9.722437534883664	3.5324510001289866
10	47.866331220925254	23.879461341967804	15.707901820641004	12.546305616465933
11	41.58417345454063	22.09655195692086	21.943865477738946	14.375409110799561
12	37.3874772013024	21.957607984155132	25.642673134484887	15.012241680057572
13	22.073334353975625	34.54796195013399	25.617526758709314	17.761176937181077
14	17.064118436295647	37.04075617538813	28.480838244672068	17.41428714364415
15	16.86514140918554	23.64287324466604	43.11058016281253	16.381405183335886
16	18.372790801682374	19.5507405884927	42.174498488927036	19.90197012089789
17	17.996655990105396	20.714248688735847	30.0940879496301	31.19500737152866
18	21.879926658413126	23.05474218937415	34.181808537954026	20.883522614258695
19	29.495228095469432	24.15643312040485	26.14210474924145	20.206234034884268
20	31.778798687952282	22.258978738895998	26.28912545823423	19.67309711491749
21	23.885995059930586	27.872913158208796	26.985243682364867	21.255848099495747
22	23.45250336631133	25.06216073187285	25.892449216014118	25.5928866858017
23	22.198945684551617	29.182405252048415	25.870726412012395	22.74792265138757
24	21.389078082751585	22.874088504152287	36.84539559733492	18.891437815761208
25	19.38149077263023	25.50503108337964	33.4248616406865	21.688616503303628
26	18.51166244546696	32.27627742021812	28.068755929469603	21.143304204845318
27	19.170941108538074	32.19025415198489	28.079508837998752	20.559295901478283
28	17.526421174068133	28.31375829824373	35.03495298013485	19.124867547553293
29	18.56750041890535	24.32435690494646	34.6195917511208	22.488550925027397
30	20.09639042219632	29.581926434193285	30.268304355530695	20.0533787880797
31	27.211633393326206	26.884923385526733	26.004366259494688	19.89907696165238
32	28.942297144331274	25.91183338295905	26.06861850440544	19.077250968304234
33	27.167705592115176	27.562862925731395	24.840737610949642	20.428693871203784
34	21.09997914514377	26.54517005146207	28.589910348227882	23.76494045516628
35	21.63788977786564	26.083542384180202	29.895303799803024	22.383264038151125
36	27.619424188981164	27.859459967717164	25.17075064222108	19.350365201080592
37	21.815746742483512	32.014518837480395	27.792869085749622	18.376865334286467
38	21.29994466832943	31.46785639804085	25.701910570036755	21.530288363592966
39	22.070923387937697	30.75973156304134	26.687802802265825	20.48154224675514
40	24.35731481068492	26.598862265126705	26.631362087317957	22.41246083687042
41	18.83205572224707	25.194185232109728	28.27593024110866	27.697828804534545
42	21.937982720606406	26.54044455802773	24.21280150637158	27.30877121499428
43	21.94639699207877	26.152833548110227	27.024904073688766	24.87586538612224
44	21.50121211317557	28.790623270885295	27.68589452264681	22.02227009329233
45	18.919260363838887	34.35327644257139	24.046107314509314	22.68135587908041
46	20.93680496369688	32.53458832152161	25.263211189775575	21.265395525005935
47	21.681624701793638	27.612914580678762	27.29825940306892	23.407201314458685
48	22.374705108716487	24.742394306262604	30.281829875003464	22.601070710017442
49	20.873010802333333	25.742004935247483	30.837003024556896	22.547981237862295
50	19.46368060486316	31.51906531668642	27.36530836858367	21.65194570986676
51	19.120190273439714	31.432728622868254	25.90370842741124	23.543372676280796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3269.0
1	5511.5
2	7754.0
3	22899.0
4	38044.0
5	28012.0
6	17980.0
7	18077.5
8	18175.0
9	19329.0
10	20483.0
11	20760.5
12	21038.0
13	21588.0
14	22138.0
15	21921.5
16	21705.0
17	20034.5
18	18364.0
19	19167.5
20	19971.0
21	20336.5
22	20702.0
23	20104.0
24	19506.0
25	21250.0
26	29712.5
27	36431.0
28	44396.5
29	52362.0
30	61748.0
31	71134.0
32	78645.0
33	86156.0
34	102215.0
35	118274.0
36	120231.0
37	122188.0
38	147439.0
39	172690.0
40	211815.5
41	250941.0
42	298162.5
43	345384.0
44	348301.0
45	351218.0
46	345341.0
47	339464.0
48	325891.0
49	312318.0
50	297427.0
51	282536.0
52	262643.0
53	242750.0
54	224589.0
55	206428.0
56	194820.0
57	183212.0
58	173672.5
59	164133.0
60	157461.5
61	150790.0
62	134604.0
63	118418.0
64	103273.5
65	88129.0
66	74083.0
67	60037.0
68	49551.0
69	39065.0
70	33522.5
71	27980.0
72	23828.5
73	19677.0
74	17588.0
75	12303.0
76	9107.0
77	7112.0
78	5117.0
79	3791.5
80	2466.0
81	1792.5
82	1119.0
83	715.0
84	311.0
85	210.0
86	109.0
87	105.5
88	102.0
89	67.0
90	32.0
91	19.5
92	7.0
93	7.0
94	7.0
95	3.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4147715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.834103908721739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43269479700533	12.735796470742846
2	7.410485753479044	2.346768028693786
3	2.5085806618893884	1.1916338059129958
4	1.4138678754893628	0.8954932341480888
5	0.8510839637140971	0.6738075958247887
6	0.6289126374949677	0.5974960830962139
7	0.4541393111323724	0.5033622329053706
8	0.3499495795163945	0.44329104039008566
9	0.2833902356585216	0.40385073943207545
>10	2.724345379831741	10.250582443276873
>50	1.1575626689655731	13.318820870732212
>100	1.7622016562753746	53.13665382479292
>500	0.020014272575924058	2.0562945890525723
>1k	0.002309339143375853	0.6274779818168296
>5k	3.0791188578344704E-4	0.3087659719383047
>10k+	1.5395594289172352E-4	0.5099050872440802
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20917	0.5043017661531711	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7119	0.1716366722400165	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5547	0.13373628612380553	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4348	0.1048288033290619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02842528958715823	0.0
2	0.0	0.0	0.0	0.08766272513902233	0.0
3	0.0	0.0	0.0	0.1335192991803921	0.0
4	0.0	0.0	0.0	0.2360094654526649	0.0
5	0.0	0.0	0.0	0.3998587173901775	0.0
6	0.0	0.0	0.0	0.6712129449588509	0.0
7	0.0	0.0	0.0	0.8486841550106504	0.0
8	0.0	0.0	0.0	1.257150021156227	0.0
9	0.0	0.0	0.0	1.4509916906055502	0.0
10	0.0	0.0	0.0	1.7317486857221385	0.0
11	0.0	0.0	0.0	1.960814568985574	0.0
12	0.0	0.0	0.0	2.167072713530221	0.0
13	4.821932075853813E-5	0.0	0.0	2.2575321592732385	0.0
14	4.821932075853813E-5	0.0	0.0	2.296565699427275	0.0
15	4.821932075853813E-5	0.0	0.0	2.3419400802610593	0.0
16	4.821932075853813E-5	0.0	0.0	2.426950742758362	0.0
17	4.821932075853813E-5	0.0	0.0	2.5274398072191557	0.0
18	4.821932075853813E-5	0.0	0.0	2.67376133606094	0.0
19	4.821932075853813E-5	0.0	0.0	2.746717168368608	0.0
20	7.23289811378072E-5	0.0	0.0	2.8204445098084125	0.0
21	7.23289811378072E-5	0.0	0.0	2.916208080834869	0.0
22	7.23289811378072E-5	0.0	0.0	3.0240264820509606	0.0
23	7.23289811378072E-5	0.0	0.0	3.14773314945699	0.0
24	7.23289811378072E-5	0.0	0.0	3.246823853615786	0.0
25	7.23289811378072E-5	0.0	0.0	3.327109022678752	0.0
26	7.23289811378072E-5	0.0	0.0	3.400233622609075	0.0
27	7.23289811378072E-5	0.0	0.0	3.4799401598229385	0.0
28	9.643864151707626E-5	0.0	0.0	3.5600806709236292	0.0
29	9.643864151707626E-5	0.0	0.0	3.6515286127421964	0.0
30	9.643864151707626E-5	0.0	0.0	3.775669254035053	0.0
31	9.643864151707626E-5	0.0	0.0	3.88329477796811	0.0
32	1.446579622756144E-4	0.0	0.0	3.984458912919523	0.0
33	1.6876762265488347E-4	0.0	0.0	4.084658661455766	0.0
34	1.6876762265488347E-4	0.0	0.0	4.1900178773131715	0.0
35	1.9287728303415253E-4	0.0	0.0	4.323778273097356	0.0
36	1.9287728303415253E-4	0.0	0.0	4.441843279974637	0.0
37	1.9287728303415253E-4	0.0	0.0	4.5553515610402355	0.0
38	2.4109660379269067E-4	0.0	0.0	4.6721628655777945	0.0
39	2.6520626417195973E-4	0.0	0.0	4.7901555434739365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	75	0.0	45.0	1
CGTATTA	50	2.1827873E-11	45.0	42
AATACGG	260	0.0	42.403843	2
TCGATAG	190	0.0	41.44737	1
TCGTTAA	45	1.9303116E-8	40.0	24
CGTTTTT	16665	0.0	39.828983	1
CGACGGT	415	0.0	39.578316	28
AGTCGAC	40	3.4611367E-7	39.375	30
TAGCATA	1540	0.0	39.15584	30
CGGTCTA	380	0.0	39.078945	31
CCCGTAA	35	6.252232E-6	38.57143	22
ACGTAAC	35	6.252232E-6	38.57143	37
GCGATAT	650	0.0	38.423073	9
AACGGGA	1080	0.0	38.125	4
GGCGATA	2155	0.0	38.00464	8
TAACGGG	1065	0.0	37.8169	3
TGGGCGA	2920	0.0	37.679794	6
GTTGATC	1645	0.0	37.613983	16
GGGCGAT	9525	0.0	37.58268	7
TCGTATC	30	1.1404353E-4	37.500004	42
>>END_MODULE