FastQCFastQC Report
Thu 26 May 2016
SRR1545394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545394_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4491113
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256990.5722189577505621No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC103390.23021019511199114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT253.8926242E-545.037
TCGTAAG1800.041.251
GTATGCG2100.040.7142871
CGGTCTA1700.039.70588331
TACGGGA5850.039.6153834
CGTTTTT178450.038.8596231
GTATACG1450.038.7931061
CGTATCA356.2523486E-638.57142639
ATAGGGC20750.037.734944
GCTACGA4550.037.58241710
TTACGAG2100.037.51
TCGAACA301.1404506E-437.49999615
GGCGATA23300.037.3712468
ACGTTAG2050.037.3170741
GACCGAT24600.037.3170749
TGGGCGA26350.037.314996
GCGTAAG2600.037.211541
ACGGGTA4300.036.6279075
ACCGTTC800.036.56250412
GGGCGAT97900.036.542397
Produced by FastQC (version 0.11.5)