##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545394_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4491113
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32178816253343	31.0	31.0	33.0	30.0	34.0
2	31.672279455003693	31.0	31.0	34.0	30.0	34.0
3	31.75201158376554	31.0	31.0	34.0	30.0	34.0
4	35.473607989823456	37.0	35.0	37.0	33.0	37.0
5	35.33193509047757	37.0	35.0	37.0	33.0	37.0
6	35.4041557181928	37.0	35.0	37.0	33.0	37.0
7	35.848795165029244	37.0	35.0	37.0	35.0	37.0
8	35.86468009154969	37.0	35.0	37.0	35.0	37.0
9	37.49658670356324	39.0	37.0	39.0	35.0	39.0
10	36.904158056143324	39.0	37.0	39.0	32.0	39.0
11	36.53857228709231	39.0	35.0	39.0	32.0	39.0
12	35.847144571958	37.0	35.0	39.0	31.0	39.0
13	35.52542766125012	37.0	35.0	39.0	30.0	39.0
14	36.522078825449285	38.0	35.0	41.0	31.0	41.0
15	36.70032528684983	38.0	35.0	41.0	31.0	41.0
16	36.84133086831705	38.0	35.0	41.0	32.0	41.0
17	36.772312787498336	38.0	35.0	41.0	31.0	41.0
18	36.66957299894258	38.0	35.0	40.0	31.0	41.0
19	36.61103094043726	38.0	35.0	40.0	31.0	41.0
20	36.47657362440001	38.0	35.0	40.0	31.0	41.0
21	36.28797249145145	38.0	35.0	40.0	31.0	41.0
22	36.20500686578138	38.0	35.0	40.0	30.0	41.0
23	36.15945201111617	38.0	35.0	40.0	30.0	41.0
24	36.081081237546236	37.0	35.0	40.0	30.0	41.0
25	35.95941629613862	37.0	34.0	40.0	30.0	41.0
26	35.86659097644615	37.0	34.0	40.0	30.0	41.0
27	35.81749824598045	37.0	34.0	40.0	30.0	41.0
28	35.77980937019398	37.0	34.0	40.0	30.0	41.0
29	35.763800643626645	37.0	34.0	40.0	30.0	41.0
30	35.655435746105695	37.0	34.0	40.0	29.0	41.0
31	35.47638636569599	37.0	34.0	40.0	29.0	41.0
32	35.30732715921421	37.0	34.0	40.0	29.0	41.0
33	35.12215635634196	37.0	34.0	40.0	27.0	41.0
34	34.91410592429983	37.0	34.0	40.0	26.0	41.0
35	34.75585227982462	36.0	34.0	40.0	25.0	41.0
36	34.61190266199047	36.0	33.0	40.0	24.0	41.0
37	34.57000614324333	36.0	33.0	40.0	25.0	41.0
38	34.52382894841435	36.0	33.0	40.0	25.0	41.0
39	34.44775226096515	36.0	33.0	40.0	24.0	41.0
40	34.27909785391728	36.0	33.0	40.0	23.0	41.0
41	34.211803399291	36.0	33.0	40.0	23.0	41.0
42	34.13601171914401	35.0	33.0	40.0	23.0	41.0
43	34.03710594678869	35.0	33.0	40.0	23.0	41.0
44	33.95927201119188	35.0	33.0	40.0	23.0	41.0
45	33.908718395640456	35.0	33.0	40.0	23.0	41.0
46	33.856503053920044	35.0	33.0	40.0	23.0	41.0
47	33.79943301359819	35.0	33.0	40.0	23.0	41.0
48	33.74605470848763	35.0	33.0	39.0	23.0	41.0
49	33.68137742247857	35.0	33.0	39.0	24.0	41.0
50	33.557486306846435	35.0	33.0	39.0	23.0	41.0
51	33.39056198318769	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	15.0
13	21.0
14	47.0
15	142.0
16	455.0
17	1238.0
18	2745.0
19	5398.0
20	9529.0
21	14756.0
22	21806.0
23	31091.0
24	43203.0
25	59170.0
26	76707.0
27	90441.0
28	101140.0
29	114750.0
30	134289.0
31	161758.0
32	198007.0
33	251610.0
34	415266.0
35	602957.0
36	335192.0
37	405649.0
38	555794.0
39	857433.0
40	494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.81765566798253	23.34672941874319	28.78999036541721	15.045624547857066
2	33.05770752149857	25.752124250714694	28.293454206117723	12.896714021669018
3	28.785470327733904	25.54409118630504	31.761703613335936	13.908734872625114
4	27.152200356570855	26.884916945977533	30.833136463054927	15.129746234396684
5	23.55752349139289	31.689004485079757	29.75278065815757	15.000691365369786
6	22.68226161310125	40.301279437858724	26.835641855370817	10.180817093669209
7	85.02400184542228	4.967588212543305	7.867737908175545	2.1406720338588676
8	85.73395948843861	4.238615238583398	8.024714586339734	2.0027106866382565
9	80.52320215501146	6.047365096358074	9.996475261254838	3.4329574873756235
10	48.964900237424445	22.863352580974915	15.782168028281632	12.38957915331901
11	41.97355978351023	22.06626731502859	21.583981520839043	14.37619138062213
12	37.34234698614798	21.270963344721007	26.524805766410243	14.861883902720773
13	23.381420151307704	32.926982687810344	26.37617891155266	17.315418249329287
14	18.14523482263751	35.54176882211603	29.33451017598533	16.978486179261132
15	17.892825230627686	23.104473211874204	43.323336553767405	15.6793650037307
16	20.487571788997517	19.334939913558173	41.48437146872056	18.69311682872375
17	20.31554316268595	19.713287107227096	30.132397024969087	29.83877270511786
18	23.68579904357784	22.903075473718875	33.479050738647636	19.93207474405565
19	30.046471776595247	23.127941781914636	27.183239433075947	19.642347008414173
20	31.865709012443016	22.204495856595013	26.56735646598071	19.362438664981262
21	25.353002696658933	26.408776621741648	27.950243068923005	20.28797761267641
22	23.56333496841429	24.596620036057875	27.32093358595074	24.51911140957709
23	22.846541603384285	27.169323060898265	27.185822311752123	22.798313023965328
24	22.752155200726413	22.257645265215995	36.00855734424852	18.981642189809076
25	21.083036654833666	23.541892622162923	33.63807590679638	21.736994816207027
26	19.260726684009065	30.595467092455703	28.657506502285734	21.486299721249498
27	19.744793773837355	30.69058827956455	29.611323518245925	19.95329442835217
28	18.11655596285375	27.923991224447036	35.17687041051962	18.782582402179592
29	20.15598360584559	24.78632802158396	34.12419148660922	20.93349688596123
30	21.51261836431192	28.32538393044219	30.42277048027961	19.73922722496628
31	28.05335782021071	25.66352705888273	27.080503207111466	19.20261191379509
32	29.883973081950955	25.324457434048085	27.30374408303688	17.487825400964084
33	28.619319977030194	27.145832224662346	25.23016009617215	19.004687702135307
34	21.9624177792899	26.87247014270182	28.6789488485371	22.486163229471178
35	23.180490003257546	25.576555299321125	30.091560822450912	21.151393874970413
36	27.959594871026404	27.92735341996516	25.34273798054068	18.770313728467755
37	22.96170236642899	32.15125070333345	27.184709001977907	17.702337928259652
38	22.184678942614003	31.656562638259157	24.45685512700304	21.7019032921238
39	23.378547812090233	30.485917410673036	25.871916382420125	20.263618394816607
40	25.26115464028627	26.1174457200253	26.166030558572007	22.45536908111642
41	19.150442217775414	25.806965890192473	27.92098528805666	27.12160660397545
42	22.288728874112053	26.341911236702348	25.429153085215177	25.940206803970415
43	22.96644506606714	25.625696792754937	26.66523866132961	24.74261947984831
44	20.96275466682758	28.703419397374326	28.692174968654765	21.641650967143335
45	20.42003841809369	32.71222523236445	24.821041020343955	22.046695329197906
46	21.705933473506455	31.558658176714772	25.857866413069548	20.87754193670923
47	22.694218560076308	26.895894180351288	27.572897854050876	22.83698940552153
48	23.07198683266264	24.765575927392607	31.055909748875166	21.106527491069585
49	22.089557755505147	25.589981815198147	30.43499462160048	21.88546580769622
50	20.66688146123244	30.77206028884154	27.1536921916683	21.40736605825772
51	20.21400931127763	31.037963195314838	26.163848471414546	22.584179021992988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3833.0
1	7351.0
2	10869.0
3	29495.5
4	48122.0
5	34581.0
6	21040.0
7	21064.5
8	21089.0
9	21719.0
10	22349.0
11	22747.5
12	23146.0
13	23315.0
14	23484.0
15	23085.5
16	22687.0
17	21232.5
18	19778.0
19	20344.5
20	20911.0
21	21747.0
22	22583.0
23	23404.0
24	24225.0
25	28040.0
26	36776.0
27	41697.0
28	48276.0
29	54855.0
30	62658.5
31	70462.0
32	84091.5
33	97721.0
34	102938.5
35	108156.0
36	117586.5
37	127017.0
38	145525.0
39	164033.0
40	220320.0
41	276607.0
42	319267.5
43	361928.0
44	364137.5
45	366347.0
46	357288.5
47	348230.0
48	325520.0
49	302810.0
50	294704.5
51	286599.0
52	272043.5
53	257488.0
54	243653.5
55	229819.0
56	224503.0
57	219187.0
58	213468.0
59	207749.0
60	198203.5
61	188658.0
62	164585.5
63	140513.0
64	122511.0
65	104509.0
66	88907.5
67	73306.0
68	63096.5
69	52887.0
70	44246.5
71	35606.0
72	30635.0
73	25664.0
74	20875.5
75	12521.0
76	8955.0
77	6675.0
78	4395.0
79	3380.5
80	2366.0
81	1640.0
82	914.0
83	661.5
84	409.0
85	247.5
86	86.0
87	62.5
88	39.0
89	26.0
90	13.0
91	15.0
92	17.0
93	9.5
94	2.0
95	5.5
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4491113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.307483919799871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41081216520318	12.461946981166955
2	6.9606979008108265	2.1310154237449286
3	2.270423633712078	1.042634197925437
4	1.1906048854892004	0.7290066055784441
5	0.7603738554977578	0.5819705283034079
6	0.542475815311858	0.4982363891859955
7	0.42448925441928287	0.4548503705305707
8	0.34332532392244935	0.420435750016238
9	0.2618030099991169	0.3606790829145018
>10	2.6778584807222074	9.858462100476624
>50	1.2964143663497136	14.453682620537878
>100	1.838411431178411	53.59029151607233
>500	0.01966791822020454	1.918376756424076
>1k	0.0023484081456960648	0.6887229585334913
>5k	0.0	0.0
>10k+	2.935510182120081E-4	0.8096887185890899
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25699	0.5722189577505621	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10339	0.23021019511199114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012869860989914972	0.0
2	2.226619548428196E-5	0.0	0.0	0.047850054095721926	0.0
3	2.226619548428196E-5	0.0	0.0	0.07926765592404378	0.0
4	2.226619548428196E-5	0.0	0.0	0.12829781838043264	0.0
5	2.226619548428196E-5	0.0	0.0	0.23143483586362668	0.0
6	2.226619548428196E-5	0.0	0.0	0.36908445634745773	0.0
7	2.226619548428196E-5	0.0	0.0	0.4459473631591991	0.0
8	2.226619548428196E-5	0.0	0.0	0.6170630754559059	0.0
9	2.226619548428196E-5	0.0	0.0	0.7132530399480039	0.0
10	2.226619548428196E-5	0.0	0.0	0.8686488182328078	0.0
11	2.226619548428196E-5	0.0	0.0	1.039630933356609	0.0
12	4.453239096856392E-5	0.0	0.0	1.1701553712854698	0.0
13	6.679858645284587E-5	0.0	0.0	1.2307862215891696	0.0
14	8.906478193712784E-5	0.0	0.0	1.2575501885612765	0.0
15	8.906478193712784E-5	0.0	0.0	1.291328007110932	0.0
16	8.906478193712784E-5	0.0	0.0	1.3651181789458426	0.0
17	8.906478193712784E-5	0.0	0.0	1.4418029561937096	0.0
18	8.906478193712784E-5	0.0	0.0	1.544717311722061	0.0
19	8.906478193712784E-5	0.0	0.0	1.5994253540269416	0.0
20	8.906478193712784E-5	0.0	0.0	1.6587870311880373	0.0
21	8.906478193712784E-5	0.0	0.0	1.7334455846468348	0.0
22	8.906478193712784E-5	0.0	0.0	1.8142718742547783	0.0
23	1.1133097742140979E-4	0.0	0.0	1.9043163687932145	0.0
24	1.1133097742140979E-4	0.0	0.0	1.978908123665559	0.0
25	1.1133097742140979E-4	0.0	0.0	2.039939765487976	0.0
26	1.1133097742140979E-4	0.0	0.0	2.10106047209233	0.0
27	1.1133097742140979E-4	0.0	0.0	2.1624261068470108	0.0
28	1.3359717290569175E-4	0.0	0.0	2.2261296921275417	0.0
29	1.3359717290569175E-4	0.0	0.0	2.2945091784597715	0.0
30	1.3359717290569175E-4	0.0	0.0	2.3824829168181694	0.0
31	1.558633683899737E-4	0.0	0.0	2.462819350125459	0.0
32	1.7812956387425568E-4	0.0	0.0	2.543244848214685	0.0
33	1.7812956387425568E-4	0.0	0.0	2.6258746996568556	0.0
34	2.0039575935853763E-4	0.0	0.0	2.709551062286787	0.0
35	2.0039575935853763E-4	0.0	0.0	2.8122204896648113	0.0
36	2.0039575935853763E-4	0.0	0.0	2.9062728993904186	0.0
37	2.0039575935853763E-4	0.0	0.0	3.0001026471611825	0.0
38	2.0039575935853763E-4	0.0	0.0	3.096715669367482	0.0
39	2.0039575935853763E-4	0.0	0.0	3.2039719330152683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAT	25	3.8926242E-5	45.0	37
TCGTAAG	180	0.0	41.25	1
GTATGCG	210	0.0	40.714287	1
CGGTCTA	170	0.0	39.705883	31
TACGGGA	585	0.0	39.615383	4
CGTTTTT	17845	0.0	38.859623	1
GTATACG	145	0.0	38.793106	1
CGTATCA	35	6.2523486E-6	38.571426	39
ATAGGGC	2075	0.0	37.73494	4
GCTACGA	455	0.0	37.582417	10
TTACGAG	210	0.0	37.5	1
TCGAACA	30	1.1404506E-4	37.499996	15
GGCGATA	2330	0.0	37.371246	8
ACGTTAG	205	0.0	37.317074	1
GACCGAT	2460	0.0	37.317074	9
TGGGCGA	2635	0.0	37.31499	6
GCGTAAG	260	0.0	37.21154	1
ACGGGTA	430	0.0	36.627907	5
ACCGTTC	80	0.0	36.562504	12
GGGCGAT	9790	0.0	36.54239	7
>>END_MODULE