FastQCFastQC Report
Thu 26 May 2016
SRR1545393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545393_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences434923
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87392.0093211901876886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG351.2095916E-745.01
ACGGGTA302.1623782E-644.9999965
CGTTTTT58100.043.1411361
TTACGGG1100.040.9090923
GTTACGG451.9235813E-840.02
CGACAGG403.4516052E-739.3750042
CGAGGGA1900.039.0789454
GTTGATC2650.039.05660216
CAGTAGC2600.038.94230727
GTAGGGC2200.038.8636364
TCGTTGA356.2393356E-638.57142624
AATGGTC700.038.57142610
TTCGTAC356.2393356E-638.57142633
CCAGTAG2650.038.20754626
AGCATAT2600.038.0769231
CATACGA1300.038.0769218
ATACGAA1300.038.0769219
ACATACG1300.038.0769217
GGTACCT2800.037.7678578
AGTAGCA2750.037.63636428
Produced by FastQC (version 0.11.5)